Isolation and purification of six iridoid glycosides from<i>gardenia jasminoides</i>fruit by medium-pressure liquid chromatography combined with macroporous resin chromatography

Wang, Yun; Liu, Hui; Shen, Lifeng; Yao, Lan; Ma, Yueming; Yu, Dao‐Yi; Chen, Jianhong; Li, Puling; Chen, Ying; Zhang, Cun

doi:10.1002/jssc.201500705

Cited by 27 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the last few years, macroporous adsorption resins, one kind of promising adsorption material with many advantages, such as low cost, high adsorption capacity, and effortless regeneration, have been widely used for the enrichment of various active ingredients . However, there are also some problems in the application of macroporous resins.…”

Section: Introductionmentioning

confidence: 99%

Purification of spinosin from Ziziphi Spinosae Semen using macroporous resins followed by preparative high‐performance liquid chromatography

Shi

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

As a well‐known traditional Chinese medicine, Ziziphi Spinosae Semen has been used for treating anxiety and insomnia for a long time. Spinosin, the main active C‐glycoside flavonoid in Ziziphi Spinosae Semen, has attracted much attention because of its many pharmacological activities including strong hypnotic effects, anxiolytic‐like effects, and so on. In the present work, high‐purity spinosin was separated from Ziziphi Spinosae Semen using the HPD‐300 resin followed by preparative high‐performance liquid chromatography. The adsorption kinetics curve of spinosin on the HPD‐300 resin was studied and fitted well by the pseudo‐second‐order equation. The adsorption isotherms were also constructed and low temperature favored the adsorption reaction. The separation parameters were optimized using dynamic adsorption and desorption tests. After a one‐run treatment with HPD‐300 resin, the concentration of spinosin increased 11.8‐fold from 0.99 to 11.7% with a recovery yield of 80.4%. Furthermore, the purity of spinosin could surpass above 98% after separation by preparative high‐performance liquid chromatography and recrystallization with a recovery yield of 72.6%. The developed method was effective and suitable for the large‐scale preparation of spinosin. Moreover, it was confirmed that HPD‐300 resin could enable good selection for the enrichment of flavonoids from different plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Purification of spinosin from Ziziphi Spinosae Semen using macroporous resins followed by preparative high‐performance liquid chromatography

Shi

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Then, these six iridoid glycosides constitute the characteristic high-content chemical constituent group of Fructus Gardeniae from Jiangxi province. Iridoid glycosides have been demonstrated to be the major bioactive ingredients in Fructus Gardeniae [ 28 – 30 ], among which, geniposide is the quality control standard recorded in the Chinese Pharmacopoeia (2000–2015 edition) [ 31 ], and gardenoside as a hydroxylation product of geniposide is also an important constituent in Fructus Gardeniae [ 5 , 6 ]. This is similar to those recorded in ancient Chinese materia medica books.…”

Section: Discussionmentioning

confidence: 99%

“…Fructus Gardeniae has been reported to protect the liver and gallbladder cells from infringement, treat cardiovascular and cerebrovascular diseases, and promote ligament cell proliferation and collagen synthesis effectively [ 5 , 6 ]. Researchers found that the chemical composition of Fructus Gardeniae includes iridoid glycosides [ 5 , 7 ], crocins [ 8 ], phenylpropanoids [ 9 ], glycoprotein [ 10 ], and polysaccharides. Among those, iridoid glycosides are the major active constituents in Fructus Gardeniae [ 11 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Study on Mechanism of Iridoid Glycosides Derivatives from Fructus Gardeniae in Jiangxi Province by Network Pharmacology

Liu

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. To investigate the pharmacological mechanism of the iridoid glycosides from Fructus Gardeniae in Jiangxi province by network pharmacology. To provide a valuable research strategy for the rational use and in-depth research and development of Fructus Gardeniae from Jiangxi. Method. Previous research results of our group show that the contents of iridoid glycosides in Fructus Gardeniae from Jiangxi province have a significant difference compared with other regions (P<0.05). Based on our previous experimental results, this study selected six characteristic high-content bioactive iridoid glycosides components of Fructus Gardeniae from Jiangxi province as candidate components. TCMSP database was used to obtain the process parameters of absorption, distribution, metabolism, and excretion (ADME) of candidate components. PubChem and SWISS online database were used to predict the related targets. Cytoscape software was used to the construct compound-target-disease (C-T-D) network of the Fructus Gardeniae iridoid glycosides ingredients. Furthermore, the GO biological process analysis and the pathway enrichment analysis were carried out using the CTD online analysis platform; then, an illustrated network that contains the main “chemicals-targets-pathway (C-T-P)” was constructed to analyze main biological pathways for obtaining the deep mechanism of Fructus Gardeniae in Jiangxi. Results. 6 iridoid glycosides, namely geniposide, gardenoside, geniposidic acid, genipin 1-gentiobioside, gardoside, and shanzhiside, from Fructus Gardeniae in Jiangxi province were obtained as candidate components through previous work and network pharmacology screening. 36 corresponding targets were acted, such as BCL2, MAPT, F2, BCL2L1, PRKCD, PRKCB, HIF1A, and PRKCA. These targets could joint in pathways, such as signaling by GPCR, neuroactive ligand-receptor interaction, inflammatory mediator regulation of TRP channels, and ion channel transport. Interestingly, these pathways were highly associated with liver diseases, neurological diseases, hypertension, neoplasms, hyperalgesia, and inflammation. Remarkably, we boldly speculate that the Fructus Gardeniae from Jiangxi province can play a pharmacological role in hepatic encephalopathy through regulating multiple signaling pathways in an integrated manner. Conclusion. The method based on system pharmacology could help to find the key targets of characteristic high-content chemical constituents of herb from different producing areas, the signaling pathway and disease network of TCM, and provide useful information and data support for giving a further study on traditional Chinese medicine resources in different regions of China.

show abstract

“…Medium‐pressure liquid chromatography (MPLC) is a modern technology that could provide high efficiency and recovery. Besides, MPLC has advantages of high sample loading, high throughput, short separation time, and ease of operation . In the present study, MPLC is selected for enrichment of the minor flavonoids from barley seedlings.…”

Section: Introductionmentioning

confidence: 99%

A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium‐pressure LC, and high‐speed countercurrent chromatography

Wang

Chen

Yang

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

Separation of minor compounds especially with similar polarities and structures from complex samples is a challenging work. In the present study, an efficient method was successfully established by macroporous resin column chromatography, medium‐pressure liquid chromatography, and high‐speed countercurrent chromatography for separation of four minor flavonoids from barley seedlings. Macroporous resin column chromatography and medium‐pressure liquid chromatography were used for enrichment of these four flavonoids. High‐pressure liquid chromatography analysis showed the total content of these four flavonoids increased from 2.2% in the crude extract to 95.3% in the medium‐pressure liquid chromatography fraction. It was indicated that the combination of macroporous resin column chromatography and medium‐pressure liquid chromatography could be a practicable strategy for enrichment of minor compounds from complex sample. Then, high‐speed countercurrent chromatography was employed for separation of these four flavonoids using ethyl acetate/n‐butanol/water (0.1% glacial acetic acid) (4:1:5, v/v/v) as solvent system. As a result, four flavonoids including two isomers with purities higher than 98% were obtained. Interestingly, two flavonoids existing in one high‐pressure liquid chromatography peak were also successfully separated. All these indicated high‐speed countercurrent chromatography had great potential for separation of compounds with similar structures and polarities. This study provides a reference for efficient enrichment and separation of minor compounds from complex sample.

show abstract

Isolation and purification of six iridoid glycosides fromgardenia jasminoidesfruit by medium-pressure liquid chromatography combined with macroporous resin chromatography

Cited by 27 publications

References 37 publications

Purification of spinosin from Ziziphi Spinosae Semen using macroporous resins followed by preparative high‐performance liquid chromatography

Purification of spinosin from Ziziphi Spinosae Semen using macroporous resins followed by preparative high‐performance liquid chromatography

Study on Mechanism of Iridoid Glycosides Derivatives from Fructus Gardeniae in Jiangxi Province by Network Pharmacology

A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium‐pressure LC, and high‐speed countercurrent chromatography

Contact Info

Product

Resources

About