Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B₁ contamination in dairy cattle feed

Abstract

The abilities of isolates to survive and be active in anaerobic and aerobic conditions rendered them to be active in cattle's rumen. Their biomass could be produced in bulk and used as feed supplement for aflatoxin detoxification in dairy cattle.

Effect of Supplemental Kluyveromyces marxianus and Pichia kudriavzevii on Aflatoxin M1 Excretion in Milk of Lactating Dairy Cows

“…Treatments consisted of control (no supplemental yeast), K. marxianus CPY1 (K1Y), K. marxianus RSY5 (K2Y) or P. kudriavzevii YSY2 (PY). These yeasts were previously identified to have the potential to effectively detoxify aflatoxin in vitro [27]. Supplemental yeasts were individually fed at the rate of 2 g/day (1 g provided 1 × 10 9 CFU) by mixing supplemental yeast with 20 g cornmeal based on our previous research [27].…”

“…These yeasts were previously identified to have the potential to effectively detoxify aflatoxin in vitro [27]. Supplemental yeasts were individually fed at the rate of 2 g/day (1 g provided 1 × 10 9 CFU) by mixing supplemental yeast with 20 g cornmeal based on our previous research [27]. Cows fed the control diet were fed 20 g corn meal without the yeast.…”

“…In our previous study, we isolated AFB 1 -detoxifying yeast from ruminal fluid of lactating dairy cows and used as a probiotic and mycotoxin biodegradation source. The results revealed that yeast isolates K. marxianus CPY1, K. marxianus RSY5 and P. kudriavzevii YSY2 can effectively detoxify AFB 1 in vitro [27]. The objectives of this study were to determine the effects of supplementing K. marxianus CPY1, K. marxianus RSY5 and P. kudriavzevii YSY2 on transferring of AFB 1 in feed into AFM 1 in cow's milk and on dry matter intake (DMI), nutrient digestibility and milk production of lactating dairy cows fed these supplements.…”

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

“…A novel approach for the microbiological detoxification of animal feed is the screening of isolates from various environmental sources (Intanoo et al, 2018), instead of using the most widespread species, S. cerevisiae. Various bacteria and yeasts may exhibit toxin-binding or even toxin-degrading abilities, as well as biocontrol effects on toxigenic molds (Pfliegler et al, 2015) and these may be directly applied to supplement animal feed (Intanoo et al, 2018). Novel yeast species in this field include members of the genera Kluyveromyces and Pichia, both related to the widely used Saccharomyces.…”

“…P. kudriavzevii has been successfully applied as a bioadsorbent feed additive to ameliorate the negative effects of AFB 1 contamination on broiler chicken performance (Magnoli et al, 2017). Novel isolates of K. marxianus have also been proposed as bioadsorbents based on in vitro characterization (Intanoo et al, 2018). However, Battacone et al (2009) found no evidence for AFB 1 detoxification in ewes fed with Kluyveromyces lactis DYP, highlighting the need for rigorous testing of novel strains in different setups and with multiple animal species.…”