Isolation and Screening of Extracellular Lipase-Producing Endophytic Fungi from Handroanthus impetiginosus

Souza, Isabela Morato de; Bassi, Gabrielle. J.; Luiz, Jaine Honorata Hortolan; Hirata, Daniela B.

doi:10.9734/ajb2t/2018/43014

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…The endophytic Aspergillus sojae isolated from the plant Plectranthus amboinicus produced stable lipases, with maximum activity at pH 6 under a temperature of 27 • C [40]. Rocha et al (2020) [2] isolated the fungi Stemphylium lycopersici from the leaves of a Humiria balsamifera and noted that under a temperature of 30 • C and pH 7 it was a great lipase producer.…”

Section: Discussionmentioning

confidence: 99%

Euterpe oleracea Mart (Açaizeiro) from the Brazilian Amazon: A Novel Font of Fungi for Lipase Production

et al. 2022

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Lipases (EC 3.1.1.3) are hydrolases that catalyze triglycerides hydrolysis in free fatty acids and glycerol. Among the microorganisms that produce lipolytic enzymes, the entophytic fungi stand out. We evaluated 32 fungi of different genera, Pestalotiopsis, Aspergillus, Trichoderma, Penicillium, Fusarium, Colletotrichum, Chaetomium, Mucor, Botryodiplodia, Xylaria, Curvularia, Neocosmospora and Verticillium, isolated from Euterpe oleracea Mart. (Açaizeiro) from the Brazilian Amazon for lipase activity. The presence of lipase was evidenced by the deposition of calcium crystals. The endophytic Pestalotiopsis sp. (31) and Aspergillus sp. (24) with Pz 0.237 (++++) and 0.5 (++++), respectively, were the ones that showed the highest lipolytic activity in a solid medium. Lipase activity was rated in liquid medium, in a different range of temperatures (°C), pH and time (days). The values obtained in the production of lipase by the endophytic fungi were 94% for Pestalotiopsis sp. (31) and 93.87% for Aspergillus sp. (24). Therefore, it is emphasized that the endophytic fungus isolated the E. oleracea palm may be a potential candidate to produce enzymes of global commercial interest.

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Section: Discussionmentioning

confidence: 99%

Euterpe oleracea Mart (Açaizeiro) from the Brazilian Amazon: A Novel Font of Fungi for Lipase Production

et al. 2022

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“…The endophytic fungus P. africana was previously isolated and identified by Souza et al [16], (GenBank access code: SAMN 06350896; CPQBA 1304/16 DRM -01). Lipases were produced by submerged fermentation.…”

Section: Microorganism and Lipase Productionmentioning

confidence: 99%

“…Lipases were produced by submerged fermentation. The inoculum medium had the following composition: 2% (m/v) casein peptone, 0.1% (m/v) yeast extract, 0.05% (m/v) sodium nitrate, 0.05% (w/v) magnesium sulfate heptahydrate and 1% (v/v) sunflower oil [16].…”

Section: Microorganism and Lipase Productionmentioning

confidence: 99%

“…The production medium had a similar composition to that described by Souza et al (2018) [16]: 2% (w/v) casein peptone, 0.1% (m/v) yeast extract, 0.05 % (m/v) sodium nitrate, 0.05% (m/v) magnesium sulfate heptahydrate, 0.05% (m/v) calcium nitrate tetrahydrate, 0.05% (m/v) monobasic sodium phosphate and 1% (v/v) sunflower oil.…”

Section: Microorganism and Lipase Productionmentioning

confidence: 99%

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Precipitation Using Organic Solvents for Purifying Lipase from Preussia africana

Ferreira,

Pereira,

Hirata

2023

BJI

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Aims: The present study aimed to purify a new lipase from the endophytic fungus Preussia africana (P. africana). Study Design: Lipases were produced through fermentation and precipitation using different organic solvents and characterized at optimal temperature and pH conditions. Place and Duration of Study: Assays were carried out at the Laboratory of Bioprocess at the Federal University of Alfenas between January and August, 2023. Methodology: Enzymes were produced via submerged fermentation. After fermentation, the broth was filtered and lyophilized so that enzymes were concentrated. Organic solvents (methanol, ethanol, isopropanol and acetone) were used at 10 and 25°C for lipase precipitation. A second precipitation step was investigated in a supernatant: solvent ration ranging from 1:3 to 1:2 (v/v) and temperatures between 10°C and 25°C. Optimal temperature and pH conditions for the hydrolytic activity of precipitated lipase were also found. Results: Acetone and isopropanol were more efficient in precipitating lipase while maintaining its catalytic activity, as specific activities of 211.34 ± 0.05 and 179.50 ± 0.08 U/mg were achieved, respectively. For the second precipitation step, acetone in a supernatant: solvent ratio of 1:2 (v/v) at 10°C achieved optimal performance, as purification factor of 1.89 ± 0.01 and recovered activity of 93.45% were found. Maximum hydrolytic activity of 72.80 ± 1.29 U/mL was observed using olive oil as substrate at pH between 7 and 8 and 37°C. Conclusion: The use of acetone at 10°C in two precipitation steps proved to be a more robust strategy for P. africana lipase precipitation in the purification process.

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Endophytic fungi isolated from medicinal plants: future prospects of bioactive natural products from Tabebuia/Handroanthus endophytes

Gómez

Luiz

2018

Appl Microbiol Biotechnol

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Medicinal plants are a rich source of natural products used to treat many diseases; therefore, they are the basis for a new drug discovery. Plants are capable of generating different bioactive secondary metabolites, but a large amount of botanical material is often necessary to obtain small amounts of the target substance. Nowadays, many medicinal plants are becoming rather scarce. For this reason, it is important to point out the interactions between endophytic microorganisms and the host plant, because endophytes are able to produce highly diverse compounds, including those from host plants that have important biological activities. Thence, this review aims at presenting the richness in bioactive compounds of the medicinal plants from Tabebuia and Handroanthus genera, as well as important aspects about endophyte-plant interactions, with emphasis on the production of bioactive compounds by endophytic fungi, which has been isolated from various medicinal plants for such a purpose. Furthermore, bio-prospection of natural products synthesized by endophytes isolated from the aforementioned genera used in traditional medicine could be used to treat illnesses.

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Isolation and Screening of Extracellular Lipase-Producing Endophytic Fungi from Handroanthus impetiginosus

Cited by 7 publications

References 29 publications

Euterpe oleracea Mart (Açaizeiro) from the Brazilian Amazon: A Novel Font of Fungi for Lipase Production

Euterpe oleracea Mart (Açaizeiro) from the Brazilian Amazon: A Novel Font of Fungi for Lipase Production

Precipitation Using Organic Solvents for Purifying Lipase from Preussia africana

Endophytic fungi isolated from medicinal plants: future prospects of bioactive natural products from Tabebuia/Handroanthus endophytes

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