Isolation and Screening of Indigenous Fungus Producing Lignin Peroxidase from the Cocoa Plantation in Sepawon Kediri Regency Indonesia

Delila, Liling; Susanti, Evi; Sanjaya, Eli Hendrik

doi:10.18502/kls.v3i5.985

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…After incubation, the appearance of a purple colour zone around the colony indicated laccase enzyme production. For lignin peroxidase activity, the method was modified from Delila et al 33 : In triplicate, the fungal plug was transferred to a PDA plate, supplemented with methylene blue (0.0065%, Sigma-Aldrich), and incubated at room temperature, in the dark, for 14 days. A clear zone around the colony, caused by the disappearance of the blue colour of the dye, confirmed the presence of peroxidase or decolorization of methylene blue dyes.…”

Section: Pectinolytic Activitymentioning

confidence: 99%

Studies on the Rapid and Simple DNA Extraction Method, Antibacterial Activity and Enzyme Activity Involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)

Tangthirasunun¹,

Poeaim²

2022

J. Pure Appl. Microbiol.

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Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp. are white mycelium and the growth rate on potato dextrose agar (PDA) result present C. sulcipes is faster than C. tricholoma. The molecular characterization from a rapid and simple DNA extraction method that is modified based on thermolysis method, The DNA extraction is finish in thirty minutes and efficiency to continuous with polymerase chain reaction (PCR) amplification to fungi species level identification. The DNA sequence from internal transcribed spacer (ITS) gene regions by universal primer pairs ITS5/ITS4 is effective to confirm Cookeina species level that C. sulcipes has 616 bp and C. tricholoma has 570 bp. Including, DNA sequence of large subunit (LSU) gene regions by universal primer pairs LROR/LR5 is generate that C. sulcipes has 912 bp and C. tricholoma has 906 bp. The cultures are screened for antibacterial activity by agar plug diffusion method and found that both isolates have been no activity against test strains (Bacillus subtilis, Escherichia coli, Kocuria rhizophila (Micrococcus luteus), Pseudomonas aeruginosa, Staphylococcus aureus and S. epidermidis). In a preliminary screening test of enzymes involved in plant biomass breakdown by agar plate method, both Cookeina sp. show cellulolytic and hemicellulolytic enzymatic activity, and manganese peroxidase (MnP) productivity. In contrast, only C. sulcipes had additional laccase activity. Neither isolate generate pectinolytic and lignin peroxidase (LiP) activities. Thus, Cookeina spp. proved the potentiality to break down lignocelluloses.

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Section: Pectinolytic Activitymentioning

confidence: 99%

Studies on the Rapid and Simple DNA Extraction Method, Antibacterial Activity and Enzyme Activity Involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)

Tangthirasunun¹,

Poeaim²

2022

J. Pure Appl. Microbiol.

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“…Indigenous WRF KLUM 2 isolated from the weathered cocoa husk of Sepawon plantation, Kediri, East Java has the potential as a source of ligninase since it can reduce methylene blue in selective media (Delila, 2016). Arinta (2017) showed that WRF KLUM 2 in Kirk's medium with a glucose carbon source (MK-Glucose) produced a LiP ligninase profile (60.67 U/mg) much higher than MnP (46.56 U/mg) and laccase (23.38 U/mg).…”

Section: Introductionmentioning

confidence: 99%

Ligninase Profiling and Optimization of Laccase Production from Indigenous Wood Rot Fungus (WRF) KLUM2 in Kirk Medium-Alkali Lignin Kayu Jati (MK-ALKJ)

Mutmainah¹,

Susanti

2021

Valensi

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The production of ligninase by wood rot fungus (WRF) is determined by carbon source and growth condition. The goal of this study is to determine the ligninase profile produced by WRF KLUM2 in Kirk Medium using teak wood alkaline lignin as a carbon source known as Kirk Medium-Alkali lignin Kayu Jati (MK-ALKJ), optimization of dominant ligninase production in the MK-ALKJ compared to the one that is produced in the Kirk’s medium with glucose as a carbon source (MK-Glucose). This research was conducted in an experimental laboratory consisting of: (1) spore suspension preparation, (2) ligninase profiling at various growth times, (3) ligninase profiling at various temperature variations, (4) optimization of laccase production including pH and the amount of nitrogen source. Growth was identified based on the specific activity of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase. The results showed that relatively the three types of ligninase, namely LiP, MnP, and laccase, were produced in the same amount by the wood rotting fungus isolates KLUM2 in MK-ALJK. All three were produced with the highest yield of respectively 55.65; 52.48; 57.64 U/mg. Laccase as the dominant ligninase can be optimized to reach 83.52 U/mg by inoculating 2.107 spore cells in MK-ALKJ in 37 °C, pH = 3.5, and a nitrogen source of 20mM (NH4)2SO4 for 6 days. Therefore, it can be concluded that the ligninase activity of indigenous WRF KLUM2 in MK-ALJK medium is higher than in the MK-Glucose.

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“…Isolat potensial penghasil ligninase adalah KPK Indigenous Mycelipotora thermophilia KLUM1. Isolat ini diisolasi oleh Delila, et al (2017) dari kulit kakao lapuk perkebunan kakao Sepawon Kabupaten Kediri Jawa Timur. Iqbal (2019) menyatakan bahwa berdasarkan urutan 16sRNAnya KLUM1 teridentifikasi sebagai Mycelioptora thermophilia dengan derajat similaritas 99%, termasuk dalam kelas Ascomycetes (Karnaouri et al, 2014;Gene, 2012).…”

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Pemanfaatan Kapang Pelapuk Kayu (KPK) Indigenous Mycelioptora thermophilia KLUM1 Untuk Pengolahan Limbah Rumput Taman Sebagai Pakan Ternak

Astiyandani

Susanti

2022

J. Ilmu Hayat

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Limbah rumput pahitan ini berpotensi menjadi pakan ternak jika dilakukan pengolahan dengan cara menurunkan kandungan ligninnya. Lignin merupakan zat antinutrisi pada pakan ternak yang keberadaannya dapat menurunkan ketercernaan nutrisi pakan. KPK Mycelioptora thermophilia KLUM1 menghasilkan enzim ligninase yaitu Lignin Peroksidase, Mangan Peroksidase dan Lakase yang dapat mendegradasi lignin pada rumput pahitan. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh waktu fermentasi dan jumlah spora terhadap kadar lignin, lemak kasar, dan protein kasar rumput pahitan oleh KPK Mycelioptora thermophilia KLUM1. Penelitian ini merupakan penelitian eksperimen laboratoris menggunakan metode Rancangan Acak Lengkap (RAL) faktor waktu fermentasi (14 dan 21 hari) dan faktor jumlah spora (1,72x10 7 , 4,29x10 7 , dan 8,59x10 7 sel). Secara umum, tahapan penelitian yang dilakukan adalah preparasi, fermentasi limbah rumput menggunakan KPK Mycelioptora thermophilia KLUM1, dan pengujian hasil fermentasi berupa kandungan lignin, lemak kasar, protein kasar. Hasil penelitian menunjukkan kondisi terbaik untuk kadar lignin dan lemak kasar pada penelitian ini yaitu pada variasi jumlah spora 8,59 x 10 7 sel selama 21 hari dengan kadar lignin sebesar 9,98% ± 0,23 dan kadar lemak kasar 3,43% ± 0,04, sementara kondisi terbaik untuk kadar protein kasar yaitu pada variasi jumlah spora 1,72 x 10 7 sel selama 14 hari sebesar 11,64% ± 0,18. Kata kunci: kapang pelapuk kayu; Mycelioptora thermophilia; lignin; limbah rumput; pakan ternak.

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Isolation and Screening of Indigenous Fungus Producing Lignin Peroxidase from the Cocoa Plantation in Sepawon Kediri Regency Indonesia

Cited by 3 publications

References 4 publications

Studies on the Rapid and Simple DNA Extraction Method, Antibacterial Activity and Enzyme Activity Involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)

Studies on the Rapid and Simple DNA Extraction Method, Antibacterial Activity and Enzyme Activity Involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)

Ligninase Profiling and Optimization of Laccase Production from Indigenous Wood Rot Fungus (WRF) KLUM2 in Kirk Medium-Alkali Lignin Kayu Jati (MK-ALKJ)

Pemanfaatan Kapang Pelapuk Kayu (KPK) Indigenous Mycelioptora thermophilia KLUM1 Untuk Pengolahan Limbah Rumput Taman Sebagai Pakan Ternak

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