Isolation and screening of proangiogenic and antiangiogenic metabolites producing rare actinobacteria from soil

Azarakhsh, Yousef; Mohammadipanah, Fatemeh; Nassiri, Seyed Mahdi; Siavashi, Vahid; Hamedi, Javad

doi:10.1111/jam.13458

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…UTMC 522 was reported recently (Azarakhsh et al . ). According to 16S rRNA analysis, Kribbella sancticallisti has a similarity percentage of 98·53% to its nearest neighbour, Kribbella sp.…”

Section: Discussionmentioning

confidence: 97%

“…The actinobacterial strains were isolated from 40 different soil samples collected from various geographical locations in Iran. Culture conditions, microbiological media and the molecular identification method were the same as previously described (Azarakhsh et al 2017). The strains were deposited in the University of Tehran Microorganisms Collection (UTMC) after isolation, purification, and morphological and taxonomic identification.…”

Section: Isolation Of Actinobacteriamentioning

confidence: 99%

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Isolation and screening of rareActinobacteria, a new insight for finding natural products with antivascular calcification activity

et al. 2017

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Aim: Vascular calcification (VC) is a significant pathological process in some life-threatening diseases. Several pathological mechanisms, including transdifferentiation of vascular smooth muscle cells to osteoblast-like cells and apoptosis are involved in VC. Compounds with an inhibitory effect on these processes are potentially efficient medications. In consideration of the multiple biological activities of Actinobacteria, this research was aimed at finding anti-VC metabolite-producing Actinobacteria. Methods and Results: After the isolation and identification of Actinobacteria, the effect of their fermentation broth extracts on the apoptosis rate was measured using various methods, for example, ethidium bromide/acridine orange staining, DNA laddering and diphenylamine assays. The effect of the most effective fermentation broth extract of Actinobacteria (FBEA) on the mRNA expression of runt-related transcription factor 2 (Runx2) and osteopontin (OPN) was examined. Finally, the most effective FBEA was fractionated and the chemical composition of anti-VC fractions was analysed using GC-MS. Various VC inhibition rates were observed in the tested FBEA (20 lg ml À1 ; 17Á9-60Á15%). The inhibition of DNA fragmentation was 7-48%.The FBE with the greatest anticalcification activity belonged to Kribbella sp. UTMC 267 and, according to 16S rRNA analysis, Kribbella sancticallisti with a similarity of 98Á53% is its nearest neighbour. The FBE of Kribbella sp. UTMC 267 reduced Runx2 mRNA expression by 2Á95-fold and OPN mRNA expression by 28Á57-fold, both of which are considered significant (P < 0Á05). Finally, GC-MS analysis showed the existence of potent anti-oxidative and anti-inflammation agents in FBE of Kribbella sp. UTMC 267. Conclusions: Actinobacterial metabolites can provide a new strategy for treating VC diseases by reducing the expression of osteogenic genes, the apoptosis rate and oxidative stress. Significance and Impact of the Study: This study highlights the therapeutic potential of Kribbella sp. metabolites and Actinobacteria as a new natural source for drug discovery programs in the nonantibiotic bioactivity field.

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“…UTMC 522 was reported recently (Azarakhsh et al . ). According to 16S rRNA analysis, Kribbella sancticallisti has a similarity percentage of 98·53% to its nearest neighbour, Kribbella sp.…”

Section: Discussionmentioning

confidence: 97%

Section: Isolation Of Actinobacteriamentioning

confidence: 99%

Isolation and screening of rareActinobacteria, a new insight for finding natural products with antivascular calcification activity

et al. 2017

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“…), anti‐angiogenic and proangiogenic agents (Azarakhsh et al . ). Among the genera of this phylum, Streptomyces is the largest genus of Actinobacteria comprising over 800 species (Parte and Euzeby ).…”

Section: Introductionmentioning

confidence: 97%

Introduction of marine-derivedStreptomycessp. UTMC 1334 as a source of pyrrole derivatives with anti-acetylcholinesterase activity

et al. 2018

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“…Marine actinomycetes are remarkable sources of novel secondary metabolites with wide chemical diversity [1]. Actinomycetales are supposed to be productive producers of a wide variety of biologically active secondary metabolites, including antibiotics, enzyme inhibitors, antivascular compounds, and antitumor compounds [2][3][4][5][6][7]. Among the many genera of marine actinomycetes, members of the genus Streptomyces have garnered increasing attention due to their capacity to produce novel compounds with unique structural and pharmacological properties [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Our intensive chemical screening of the marine actinomycete strain Streptomyces sp. CNH-189 also resulted in the discovery of novel meroterpenoids, such as ansalactams A-D, merochlorins A-F, and meroindenon [5][6][7][8]. Ansalactams possess a distinctive spiro γ-lactam moiety with a modified polyketide chain, which exhibits biosynthetic plasticity between strains [16].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Meroterpenoids, Merochlorins G–J from the Marine Bacterium Streptomyces sp.

et al. 2021

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Four new chlorinated meroterpenoids, merochlorins G−J (1−4), and 10, a dihydronaphthalenedione precursor, along with known merochlorins A (5) and C−F (6−9), were obtained from cultivation of the bacterium strain Streptomyces sp. CNH-189, which was isolated from marine sediment. The planar structures of compounds 1−4 and 10 were elucidated by interpretation of MS, UV, and NMR spectroscopic data. The relative configurations of compounds 1−4 were determined via analysis of nuclear Overhauser effect (NOE) spectroscopic data, after which their absolute configurations were established by comparing the experimental electronic circular dichroism (ECD) spectra of compounds 1−4 to those of previously reported possible enantiomer models and DP4 calculations. Compound 3 displayed strong antibacterial activities against Bacillus subtilis, Kocuria rhizophila, and Staphylococcus aureus, with MIC values of 1, 2, and 2 μg/mL, respectively, whereas compound 1 exhibited weak antibacterial effects on these three strains, with a 16−32 μg/mL MIC value range.

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Isolation and screening of proangiogenic and antiangiogenic metabolites producing rare actinobacteria from soil

Abstract: Metabolites of the introduced rare actinobacteria are potent proangiogenic and angiogenic inhibitors. Identification of angiogenic-antiangiogenic mechanisms and purification of the extracts would be useful in therapeutic angiogenesis.

Cited by 9 publications

References 44 publications

Isolation and screening of rareActinobacteria, a new insight for finding natural products with antivascular calcification activity

Isolation and screening of rareActinobacteria, a new insight for finding natural products with antivascular calcification activity

Introduction of marine-derivedStreptomycessp. UTMC 1334 as a source of pyrrole derivatives with anti-acetylcholinesterase activity

Antibacterial Meroterpenoids, Merochlorins G–J from the Marine Bacterium Streptomyces sp.

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