Isolation and Some Properties of Arachin Subunits

Process of Denaturation of Arachin by Heat Treatment

“…This subunit composition is the same as that of the intact arachin. 7 ) Moreover, the 9Scomponent exhibited the same disc electrophoretic mobility as that of the intact arachin. The 9S component was, therefore, assumed to be in a native state with respect to its subunit composition and electrophoretic behavior, while the precipitate contained the six subunits in the molar ratio of about 3/4 : 3/4 : 3/4 : 1 : 1 : 1.…”

Subunit Structure and Functional Properties of the Predominant Globulin of Perilla (Perilla frutescensvar.frutescens) Seeds

“…20) Perilla globulin also demonstrated high aspartic acid and glutamic acid contents. Such high concentrations of acidic amino acids appear to be common to plant storage proteins, including sesame -globulin, 20) the 7S globulin of soybean, 22) and the 11S globulin of soybean, 20) peanut 23) and legumin.…”

Application of HPLC, Gel electrophoresis and size exclusion chromatography for separation and characterization of storage proteins of soybean and peanut

“…Retention time and peak ratio of all these 12 subunits are also summarized in Table V. There has been no earlier report with regard to 12 subunits of arachin on the basis of slab gel electrophoresis as well as RP-HPLC, although arachin was reported to contain 12 subunits by osmometry [37,40].…”

“…Since the first evidence for a total of six differently sedimenting proteins based on sedimentation constants determined by ultracentrifuge [35], several conflicting results have been reported in literature on arachin; these included whether the number of subunits in arachin is 12 [36] or six [37], or nine [38], whether the dissociation-association of arachin species is reversible [39] or there is an existence of different dissociation-association systems between dimmer and monomer [40], and whether the subunit composition of the two species (arachin I and arachin II) is the same, while containing four different kinds of subunits, with respect to molecular weight and six different kinds of subunits with respect to isoelectric points [37] or all the six subunits were distinguishable on PAGE [41]. The addition of SDS to arachin led to its dissociation into species of approximately one-sixth of its molecular weight [42] and later into several subunits [43].…”