Isolation and structural characterisation of rhamnogalacturonan oligomers generated by controlled acid hydrolysis of sugar-beet pulp

Renard, Catherine M.G.C.; Lahaye, Marc; Mutter, M.; Voragen, Fons; Thibault, Jean-François

doi:10.1016/s0008-6215(97)10028-3

Cited by 79 publications

(48 citation statements)

References 15 publications

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“…The final beet cell walls contained enough calcium to neutralize about two-thirds of the free carboxyl groups of pectins. A procedure developed for Solid-State NMR Spectroscopy of Beet Cell Wallspectin fractionation (Renard et al, 1998) made it possible to obtain a residue containing more than 80% of cellulose while avoiding concentrated alkali extractions that might lead to mercerization. A controlled-acid hydrolysis first solubilized the "hairy regions" of pectins as oligomers, leaving a solid consisting essentially of Glc and GalUA (Renard et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…A procedure developed for Solid-State NMR Spectroscopy of Beet Cell Wallspectin fractionation (Renard et al, 1998) made it possible to obtain a residue containing more than 80% of cellulose while avoiding concentrated alkali extractions that might lead to mercerization. A controlled-acid hydrolysis first solubilized the "hairy regions" of pectins as oligomers, leaving a solid consisting essentially of Glc and GalUA (Renard et al, 1998). The residual GalUA was present as homogalacturonans, which were insoluble under acidic conditions but became readily soluble upon careful neutralization.…”

Section: Discussionmentioning

confidence: 99%

“…Cellulose was isolated from de-esterified beet pulp after controlled-acid hydrolysis (0.1 m HCl for 72 h at 80°C; Renard et al, 1998), followed by neutralization to and extensive washing at pH 7, and was dried by solvent exchange (ethanol followed by acetone). Beet arabinan was from British Sugar (Norwich, UK).…”

Section: Cell Wallsmentioning

confidence: 99%

“…After controlled-acid hydrolysis (Renard et al, 1998), repeated washings of the beet cell walls at pH 7 removed most of the homogalacturonans. However, at that point the solid residue was very fine and gave stable suspensions.…”

Section: Chemical Characterizationmentioning

confidence: 99%

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A Cross-Polarization, Magic-Angle-Spinning,13C-Nuclear-Magnetic-Resonance Study of Polysaccharides in Sugar Beet Cell Walls1

Renard

Jarvis

1999

Plant Physiology

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Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T 1 decay and crosspolarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of approximately 3 nm, mobile pectic galacturonans, and highly mobile arabinans. A direct-polarization, magic-angle-spinning spectrum recorded under conditions adapted to mobile polymers showed only the arabinans, which had a conformation similar to that of beet arabinans in solution. These cell walls contained very small amounts of hemicellulosic polymers such as xyloglucan, xylan, and mannan, and no arabinan or galacturonan fraction closely associated with cellulose microfibrils, as would be expected of hemicelluloses. Cellulose microfibrils in the beet cell walls were stable in the absence of any polysaccharide coating.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Cell Wallsmentioning

confidence: 99%

Section: Chemical Characterizationmentioning

confidence: 99%

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A Cross-Polarization, Magic-Angle-Spinning,13C-Nuclear-Magnetic-Resonance Study of Polysaccharides in Sugar Beet Cell Walls1

Renard

Jarvis

1999

Plant Physiology

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“…Oligosaccharides 5 and 6 (Table I) were prepared from 3 and 4, respectively, by treatment with a RG-rhamnohydrolase from Aspergillus aculeatus (Mutter et al, 1994). Preparation of a mixture of oligosaccharides 7, 8, 9, and 10 (Table I), and the purified hexasaccharide 7, generated by treatment of MHR-S pared by controlled acid hydrolysis of sugar-beet cell walls and isolated by ion-exchange chromatography and SEC (Renard et al, 1995(Renard et al, , 1998.…”

Section: Substratesmentioning

confidence: 99%

Rhamnogalacturonan α-d-Galactopyranosyluronohydrolase1

et al. 1998

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A new enzyme, rhamnogalacturonan (RG) ␣-D-galactopyranosyluronohydrolase (RG-galacturonohydrolase), able to release a galacturonic acid residue from the nonreducing end of RG chains but not from homogalacturonan, was purified from an Aspergillus aculeatus enzyme preparation. RG-galacturonohydrolase acted with inversion of anomeric configuration, initially releasing ␤-Dgalactopyranosyluronic acid. The enzyme cleaved smaller RG substrates with the highest catalytic efficiency. A Michaelis constant of 85 M and a maximum reaction rate of 160 units mg ؊1 was found toward a linear RG fragment with a degree of polymerization of 6. RG-galacturonohydrolase had a molecular mass of 66 kD, an isoelectric point of 5.12, a pH optimum of 4.0, and a temperature optimum of 50°C. The enzyme was most stable between pH 3.0 and 6.0 (for 24 h at 40°C) and up to 60°C (for 3 h).

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