Isolation and structure of tunichrome B-1, a reducing blood pigment from the tunicate Ascidia nigra (Linnaeus)

Bruening, Reimar C.; Oltz, Eugene M.; Furukawa, Yoshihiro; Nakanishi, Koji; Kustin, Kenneth

doi:10.1021/ja00304a059

Cited by 116 publications

(46 citation statements)

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“…After that, Kustin's group (Macara et al, 1979a;1979b) guided by fluorescence measurements reported the successful isolation of a reducing agent from the blood cells of A. nigra and C. intestinalis. nigra under anaerobic conditions and verified its chemical structure (Bruening et al, 1985). Thereafter, Nakanishi's group isolated a tunichrome B-1 from the blood cells ofA.…”

Section: Fluorescence Derived From the Blood Cellsmentioning

confidence: 91%

Vanadium in Biological Systems

Chasteen¹

1990

104

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Section: Fluorescence Derived From the Blood Cellsmentioning

confidence: 91%

Vanadium in Biological Systems

Chasteen¹

1990

104

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“…Tunichromes, which are a class of hydroxy-dopa-containing tripeptides, have been isolated from some ascidian species (74,75,84,85). Because tunichromes contain pyrogallol and cathecol moieties that can have a reducing functionality, it was proposed that tunichromes participate in the reduction of vanadium in ascidians (86).…”

Section: Redox Mechanism Of Vanadiummentioning

confidence: 99%

Advances in research on the accumulation, redox behavior, and function of vanadium in ascidians

Michibata

Ueki

2010

BioMolecular Concepts

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The discovery of high levels of vanadium-containing compounds in ascidian blood cells goes back to 1911. Ascidians, which are also known as tunicates or sea squirts, belong to a subphylum of the Chordata, between the vertebrates and invertebrates. This discovery attracted the attention of an interdisciplinary group of chemists, physiologists, and biochemists, in part because of interest in the possible role of vanadium in oxygen transport as a prosthetic group in respiratory pigments, which was later shown not to be such a role, and in part because of the fact that high levels of vanadium were unknown in other organisms. The intracellular concentration of vanadium in some ascidian species can be as high as 350 mm, which is 107 times that in seawater. Vanadium ions, which are thought to be present in the +5 oxidation state in seawater, are reduced to the +3 oxidation state via the +4 oxidation state and are stored in the vacuoles of vanadium-containing cells called vanadocytes, where high levels of protons and sulfate ions are also found. Recently, many proteins and genes that might be involved in the accumulation and reduction of vanadium have been isolated. In this review, we not only trace the history of vanadium research but also describe recent advances in our understanding of the field from several viewpoints: (i) vanadium-accumulating blood cells, (ii) the energetics of vanadium accumulation, (iii) the redox mechanism of vanadium, (iv) the possible role of sulfate, and (v) the physiological roles of vanadium.

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“…At that time Koji was confronted with the problem of isolating tunichromes from tunicate blood, and Dr. Reimar Bruening was heading the project. Tunichromes are extremely labile compounds and decompose extensively during adsorption chromatography, but they could finally be separated thanks to CCCC 3, 4. In these two articles R. Bruening named his Sanki instrument the “CCCC” or “C 4 ”.…”

Section: Story Of Cpc: a Rapid Surveymentioning

confidence: 99%

Centrifugal partition chromatography: A survey of its history, and our recent advances in the field

Marchal

Legrand

Foucault

2003

The Chemical Record

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Centrifugal partition chromatography (CPC) was introduced in 1982 by Sanki Eng. And for almost 20 years this company remained the only one manufacturing such an apparatus. A CPC instrument or a CPC column is a series of channels linked in cascade by ducts and aligned in cartridges or disks in a circle around a rotor; setting the rotor in motion submits this assembly to a constant centrifugal field. The originality of CPC is that it uses any biphasic liquid-liquid system as mobile and stationary phases. The United States branch of Sanki contributed greatly to the worldwide acceptance of the technique. Recent works performed in the Netherlands and in France have, by means of visualization of flow-patterns in CPC channels, contributed to a better knowledge of hydrodynamics and mass transfer phenomena. Nowadays research in our laboratory focuses on speediness and scale-up of the technique.

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Isolation and structure of tunichrome B-1, a reducing blood pigment from the tunicate Ascidia nigra (Linnaeus)

Cited by 116 publications

References 1 publication

Vanadium in Biological Systems

Vanadium in Biological Systems

Advances in research on the accumulation, redox behavior, and function of vanadium in ascidians

Centrifugal partition chromatography: A survey of its history, and our recent advances in the field

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