Isolation and transcriptional characterization of a morphological modifier: the Aspergillus nidulans stunted (stuA) gene

Miller, Karen Y.; Toennis, Tina M.; Adams, Tom; Miller, Bruce L.

doi:10.1007/bf00259682

Cited by 87 publications

(88 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The process of conidiation has been studied most thoroughly in the filamentous fungus A. nidulans (28)(29)(30). Several key regulatory genes have been identified and cloned and shown to control progression through the various stages of conidiation (28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mutants of Neurospora crassa that alter gene expression and conidia development.

Madi

Ebbole²,

White³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Several genes have been identied that are highly expressed during conidiation. Inactivation ofthese genes has no observable phenotypic effect. Transcripts of two such genes, con-6 and con-10, are norally absent from vegetative mycella. To identify regulatory genes that affect con-6 and/or con-10 expression, strains were prepared in which the regulatory regions for these genes were fused to a gene conferrin hygromycin resistance. Mutants were then selected that were resistant to the drug during mycelial growth. Mutations in several of the isolates had trans effects; they activated transcription of the corresponding intact gene and, in most oates, one or more ofthe other con genes. Most Analyses of the protein and mRNA species present in mycelia, aerial hyphae, and conidia have revealed significant differences in gene expression (3)(4)(5). A differential screening procedure was used to clone genes that are preferentially expressed during conidiation (4). Four of these genes, con-6, con-8, con-10, and con-13, have been sequenced, and their expression has been examined (6-10). A fifth conidiationspecific gene, eas, also has been characterized (11, 12); eas encodes the rodlet protein that coats the surface ofthe mature conidium.con-6 and con-10 encode 93-and 86-residue polypeptides, respectively; the functions of these polypeptides are unknown. con-6 and con-10 transcripts and proteins are normally undetectable in vegetative mycelium but appear at high levels during conidiation and in mature conidia; these transcripts and proteins disappear a few hours after conidia germinate (6,13). The expression pattern of these genes closely parallels that of eas (11, 12). The con-6 and con-10 proteins are present in a second type of asexual spore, the microconidium (10,13), and the con-10 protein is present in sexual spores (10, 13). Expression of con-6 and con-10 can also be induced in vegetative mycelial cultures by light exposure or by imposing conditions that induce circadian rhythm (10,(13)(14)(15). We describe here the selection and partial characterization of mutants that express con-6 and con-10 aberrantly during vegetative mycelial growth. Some of these mutants were found to be blocked in conidiation, whereas others exhibited different developmental abnormalities. MATERIALS AND METHODSStrains and Pids. Strain CH10 was constructed by homologous integration, using pCH10, a plasmid containing a con-10 translational fusion to the hygromycin phosphotransferase gene (hph) (16). Integration was at the his-3 locus of Fungal Genetics Stock Center (FGSC) strain 462: his-3 A. The integrated plasmid consisted of pBluescript KS+ (Promega) vector containing con-10 DNA from positions 1139 to 1935 (8); this segment contains the first 40 codons of con-10. The con-10 DNA is fused in-frame to the second codon of hph followed by the Aspergillus nidulans trpC transcription termination region from plasmid pDH25 (16). The plasmid also contains a 5' truncated copy of the his-3 gene that allows reconstitution of an intact copy of the ...

show abstract

Section: Discussionmentioning

confidence: 99%

“…Several key regulatory genes have been identified and cloned and shown to control progression through the various stages of conidiation (28)(29)(30)(31). Properties of the corresponding regulatory proteins and the sites at which some of them act have been determined (32,33).…”

Section: Discussionmentioning

confidence: 99%

Mutants of Neurospora crassa that alter gene expression and conidia development.

Madi

Ebbole²,

White³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Although StuA is not required for vegetative growth, we found that stuA was transcriptionally activated during the acquisition of developmental competence. Transcriptional activation was induction independent and under the control of unknown genetic loci {Miller et al 1991).…”

mentioning

confidence: 99%

StuA is required for cell pattern formation in Aspergillus.

Miller¹,

Wu²,

Miller³

1992

Genes Dev.

190

210

View full text Add to dashboard Cite

The stunted (stuA) gene product is required for the orderly differentiation and spatial organization of cell types of the Aspergillus nidulans conidiophore. Expression of the stuA gene is complex. Two transcripts, stuA~ and stuA~, are initiated from separate promoters. Transcription of both RNAs increases -50-fold during the establishment of developmental competence. Induction-dependent transcriptional and post-transcriptional regulatory mechanisms further enhance expression 15-fold. Consistent with the latter observation, both transcripts have structural features characteristic of RNAs subject to translational control. Conidiophore morphogenesis requires regulatory interactions between the products of the stuA, bristle (brlA), and abacus (abaA) genes. Enhanced stunted expression is cell type specific and dependent on a functional BrlA protein.StuA affects the spatial localization of AbaA by acting directly, or indirectly, to repress abaA expression.

show abstract

“…Em ensaios de localização celular, a proteína MAS1/GAS2 fusionada a green fluorescent protein -GFP (GAS2::GFP) e foi observada no citoplasma do apressório de M. grisea (Adams et al, 1988). Além disso, a expressão de masA está reprimida em mutante para o fator de transcrição stuA (Twumasi-Boateng et al, 2009), que esta envolvido em um complexo controle genético e morfológico do conidióforo de A. nidulans (Miller et al, 1991).…”

Section: Lista De Abreviaturas E Siglasunclassified

“…Todos os resultados da expressão genica relatica apresentaram significância estatística (p<0,05). (Aguirre et al, 1993;Busby et al, 1996;Miller et al,1991;Sheppard et al, 2005 . O apressório é uma estrutura de função adesiva que auxilia o conídio do fungo fito patogênico a se fixar na superfície da planta.…”

Section: -Tolerância a Ph E Temperaturaunclassified

Caracterização funcional do gene codificador de uma proteína com peptídeo sinal, masA, de Aspergillus fumigatus

Cocio¹

View full text Add to dashboard Cite

El gen MAS1/GAS2, conocido como "Magnaporthe Apressoria Specific", se identificó como altamente expresado durante la formación de la appressorium fito patógena hongo filamentoso Magnaporthe grisea. En Aspergillus fumigatus, hongo saprofito oportunista, se identificó masA gen ortólogo de MAS1/GAS2 como upregulated en un análisis de transcriptómica expuesto a voriconazol y anidulafungina, antifúngicos que afectan la síntesis de ergosterol y la pared celular, respectivamente. En ambos ortólogos tener una estructura en el extremo N-terminal de la proteína llamada región del péptido señal. Durante la caracterización fenotípica y funcional de gen masA de A. fumigatus se determinó en un análisis in silico de la presencia de péptido señal en la región N-terminal de la proteína. En la caracterización fenotípica de una cepa de A. fumigatus masA -deletado ningún cambio estructural de conidióforos y su aspecto macromorfológico no fue identificado. La cepa deletado masA caracterización es resistente a voriconazol y farnesol que inhiben la síntesis de ergosterol y una molécula de detección de quórum, respectivamente. Al evaluar el nivel de expresión génica masA contra diferentes clases de drogas foi observado el gen súper se expresa cuando se produce daño en la pared celular y la membrana del ADN, la síntesis de lípidos y ácidos grasos, y el estrés oxidativo. En la presencia de daños en la pared celular fúngica causada por anidulafungina, proteína MasA se encuentra cerca de en la pared celular la punta de la hifa y, además, era capaz de transferir al medio extracelular de la proteína fusionada a ella, GFP. Por lo tanto, posiblemente, MasA participa en la vía secretora del hongo sobre todo en momentos de estrés como la ruptura de la pared celular. La capacidad de secreción natural de los hongos filamentosos se ha explotado en el contexto industrial durante décadas. Indicando para este fin, una posible aplicabilidad para este péptido señal.

show abstract

Isolation and transcriptional characterization of a morphological modifier: the Aspergillus nidulans stunted (stuA) gene

Cited by 87 publications

References 27 publications

Mutants of Neurospora crassa that alter gene expression and conidia development.

Mutants of Neurospora crassa that alter gene expression and conidia development.

StuA is required for cell pattern formation in Aspergillus.

Caracterização funcional do gene codificador de uma proteína com peptídeo sinal, masA, de Aspergillus fumigatus

Contact Info

Product

Resources

About