Isolation, biochemical and molecular characterization of 2-chlorophenol-degrading Bacillus isolates

Al‐Thani, Roda; Abd‐El‐Haleem, Desouky; Al-Shammri, Mona

doi:10.5897/ajb2007.000-2428

Cited by 10 publications

(3 citation statements)

References 45 publications

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“…The presence of a clear area surrounding the colony is a signi cant indication of biosurfactant production. Bacteria with positive hemolysis were selected for supplementary tests [13].…”

Section: Hemolytic Activitymentioning

confidence: 99%

Structural characterization of biosurfactant produced by marine bacterium Pseudomonas fragi strain F1 (isolated from Persian Gulf) and evaluation of antimicrobial and antibiofilm activity

Amirinejad,

Shekarchizadeh,

Mousavi

et al. 2023

Preprint

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The marine environment is a rich source of bioactive compounds, including biosurfactant-producing bacteria that exhibit unique characteristics and functionalities. In this study, we focused on glycolipid biosurfactants produced bacteria in a commensal relationship with marine organisms. We isolated and characterized a biosurfactant-producing strain, Pseudomonas fragi strain F1, which displayed high hemolytic activity (27mm), oil spreading ability (4mm), emulsification index (40%), and decreasing surface tension (31.3 mN/m). Fourier transform infrared (FT-IR) analysis revealed the glycolipid nature of the produced biosurfactant. Elemental analysis using CHNS and Energy Dispersive Spectroscopy (EDS) confirmed the presence of carbon, hydrogen, nitrogen, sulfur, chlorine, potassium, oxygen, and some other elements in the biosurfactant. The critical micelle concentration (CMC) of the biosurfactant was measured at 350 mg.L− 1, indicating its high efficiency. Furthermore, the biosurfactant demonstrated significant antimicrobial activity against Gram-positive and Gram-negative bacteria (the largest obtained ZOI was associated to P. aeruginosa with 27 mm), making it a potential alternative to synthetic drugs. The biosurfactant also exhibited substantial inhibition of biofilm formation and disruption, as well as enzymatic activity reduction in treated bacteria. Moreover, the mixture of biosurfactant and F1 bacterium enhanced the degradation of crude oil (86%), indicating its potential for environmental remediation. These findings highlight the importance of exploring commensal biosurfactant-producing strains in marine environments for hydrocarbon degradation, combating antibiotic resistance, and disrupting microbial biofilms.

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“…The presence of a clear area surrounding the colony is a signi cant indication of biosurfactant production. Bacteria with positive hemolysis were selected for supplementary tests [13].…”

Section: Hemolytic Activitymentioning

confidence: 99%

Structural characterization of biosurfactant produced by marine bacterium Pseudomonas fragi strain F1 (isolated from Persian Gulf) and evaluation of antimicrobial and antibiofilm activity

Amirinejad,

Shekarchizadeh,

Mousavi

et al. 2023

Preprint

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“…Saudi Arabia Sabkhas showed that the most commonly isolated bacterial species were Bacillus subtilis and Lactobacillus murinus (Alotaibi et al, 2020). Bacillus spp., on the other hand, were activated at soil polluted with chlorophenol compounds at the industrial zone at Umm-Saied city (Qatar) (Al-Thani et al, 2007;Personal observation). Moreover, most isolates were Gram-positive at rhizosphere soils; few isolates were found Gram-negative, such as the rhizosphere of A. macrostachyum and both rhizosphere and non-rhizosphere of Limonium axillare (Supplementary Figure 3).…”

Section: Bacterial Characterizationmentioning

confidence: 99%

“…However, with the new development of the modern approach, as well as growing awareness among scientists of the role of microorganisms in solving many problems facing mankind, during the last decade, great efforts have been paid to the microbiology of desert soil. Some data (personal observations) indicate that the following bacteria species are present in the raw Qatari soils: ( 1 Other species can also be found, especially when the soil is exposed to various abiotic and biotic factors or treated with organic and inorganic agents from oil and gas activities (such as Bacillus spp., Pseudomonas geniculata and Achromobacter xylosoxidans) (Al-Thani et al, 2007, and some of the above species might be activated and/or thrived under those conditions.…”

Section: Sand Dunesmentioning

confidence: 99%

Microbial Ecology of Qatar, the Arabian Gulf: Possible Roles of Microorganisms

Al‐Thani

Yasseen²

2021

Front. Mar. Sci.

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The Arabian Gulf ranks among the world’s most arid and warm regions; the land has high salinity levels with many Sabkhas and receives little precipitation. This region holds about one-third of the world’s oil supply. Qatar is the leading gas producer worldwide, which raises many concerns about the pollution of the sea, groundwater, and soil. Thus, the Arabian Gulf area has paid particular attention to environmental studies since the environmental status of this region imposed unique biological diversity, and microbial ecology has gained special importance following the identification of promising roles of microorganisms. This review article discusses the microbial ecology at the main habitats of the State of Qatar. We discuss important principles for successful ecological restoration and future perspectives of using biological approaches to solve many problems related to health, the economy, and agriculture. There are at least five microbial communities that have been recognized at the Qatari habitats: marine environment, salt marshes and mangrove forests, the arid lands (including dune communities), wetlands (including pond communities), and Rawdahs (including the Ghaf tree communities). Although, the environmental conditions of this region are almost the same, these habitats are compared with those at other countries of the Arabian Gulf whenever necessary, as each habitat has its own peculiar characteristics. Some case studies are presented to describe the biochemical characterizations of bacterial isolates from soils and leaf surface of native plants, including halophytes and xerophytes at these habitats. These studies rarely went beyond the general identification at species levels. There is a discussion about the possible roles of microorganisms at the rhizosphere, non-rhizosphere, and phyllosphere, and using plant exudates to control microbial activity. However, modern approach (culture-independent methods) addressing these topics has opened the door for deeper investigations, and to explore the roles played by microorganisms at these habitats. These methods have already begun during the last decade as serious step to solve many environmental issues. In the future, it is very likely that microorganisms will be used to tackle many pollution issues, as well as health, agricultural, and economic problems.

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