Isolation, Characterization and Antagonistic Activity of  Fluorescent Pseudomonads

nka, Priya; Goudar, Geeta; Nath, Paras; Patil, P.V.

doi:10.20546/ijcmas.2017.612.449

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…All the tested isolates of P. fluorescens produced HCN, among these isolates, PFP-4 and PFP-11 showed higher level of HCN production, with brick red colour (Shruthi, 2017). Out of the 52 antagonistic isolates, 47 isolates produced HCN showing very good biocontrol potential against the phytopathogens (Priyanka et al, 2017).…”

Section: Discussionmentioning

confidence: 95%

“…The Pseudomonas isolate CA/RN was found as an efficient antagonist against S. rolfsii in dual culture technique showing the inhibition upto 94 per cent (Rakh et al, 2011). Several authors also reported that P. fluorescens inhibited S. rolfsii (Priyanka et al, 2017).…”

Section: Discussionmentioning

confidence: 96%

“…Nirmala and Reddy (2014) isolated fifty five isolates of P. fluorescens from the rhizosphere soil of groundnut fields. Similarly, Priyanka et al, (2017) isolated 62 P. fluorescence isolates on King's B medium from the 37 rhizosphere soil samples collected from major soybean growing areas of Dharwad (15) and Belagavi (22) districts of Karnataka using serial dilution and spread plate technique.…”

Section: Discussionmentioning

confidence: 99%

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Exploitation of indigenous fluorescent pseudomonads against stem and pod rot of groundnut caused by <i>Sclerotium rolfsii</i>

Sunkad

Khadarbi

Joshi

et al. 2023

JBC

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Stem and pod rot of groundnut is an economically important soil borne disease caused by Sclerotium rolfsii Sacc. The present study aimed to evaluate the indigenous bacterial bio control agent Pseudomonas fluorescens strains having multiple traits related to bio control and plant growth promoting activity. Healthy rhizospheric soil from groundnut field was collected from different North Karnataka districts. Nineteen strains of P. fluorescens were isolated by serial dilution technique on King’s B medium. The antagonistic potential of nineteen strains was tested against S. rolfsii by using dual culture technique. The per cent inhibition was highly variable among the nineteen strains of P. fluorescens ranging from 2.59 to 75.18 per cent. Maximum mycelial inhibition of S. rolfsii was recorded by the strain PF-2 (75.18%) followed by PF-3 (72.96%), PF-6 (69.62%) and least inhibition was recorded by PF-13 (2.59%). Five superior strains of P. fluorescens showed fluorescens under UV light, yellowish green pigmentation, rod shaped cells under microscope. Five superior strains were subjected for various biochemical tests and all the isolates were positive for biochemical characterization such as Gram staining, endospore production, catalase, starch hydrolysis, urease test, casein hydrolysis and gelatin liquefy action and negative for KOH and in dole test. Further, these strains were subjected for plant growth promoting traits such as HCN production, IAA production, siderophore production and volatile compounds production. Among these, the strainsviz., PF-2 and PF-3 were scored as strong with respect to antagonism and growth promotion. The strainsPF-6, PF-7 and PF-10 were scored as moderate with light brown colour. Among the tested strains of P. fluorescens, the isolates PF-2 and PF-3 were recorded higher production of siderophore, isolates PF-6, PF-7 and PF-10 showed moderate production of siderophore. The strain PF-2 of P. fluorescens showed highest per cent of mycelial inhibition of S. rolfsii indicating higher production of volatile compounds, followed by PF-3 and PF-6. Whereas, the least mycelial inhibition was recorded by the isolate PF-10 indicating less production of volatile compounds.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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