Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line

Krithika, Rajesh; Mohankumar, R.; Verma, Richa; Shrivastav, Pranav S.; Mohamad, Illiyas L.; Gunasekaran, Palani; Narasimhan, S.

doi:10.1016/j.cbi.2009.06.014

Cited by 108 publications

(81 citation statements)

References 40 publications

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“…In this study, two different assays were executed to prove the mode of cell death induced by DAM and NDAM against H400 OSCC cells; DNA fragmentation and FITC-annexin V/PI flow cytometry. Creation of DNA ladder has been broadly used as a unique indicator of programmed cell death (Krithika et al 2009). Various caspase substrates are associated in the regulation of DNA structure, repair as well as replication.…”

Section: Discussionmentioning

confidence: 99%

Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia

et al. 2016

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Oral cancer is the eleventh most prevalent cancer worldwide. The most prevalent oral cancer is oral squamous cell carcinoma (OSCC). Damnacanthal (DAM) and nordamnacanthal (NDAM), the anthraquinone compounds, are isolated from the root of Morinda citrifolia L. (Noni), which has been used for the treatment of several chronic diseases including cancer. The objectives of this study were to evaluate the cytotoxicity, cell death mode, cell cycle, and the molecular mechanism of apoptosis induced by DAM and NDAM on OSCC. The cytotoxic effects of these compounds against OSCC cell lines were determined by MTT assay. The cell death mode was analysed by DNA laddering and FITC-annexin V/PI flow cytometric assays. In addition, the mechanism of apoptosis induced by DAM and NDAM was detected using mitochondrial membrane potential, Cytochrome c, and caspases assays. Finally, the effect of DAM and NDAM on cell cycle phase distribution of OSCC cells was detected by flow cytometry. In the present study, DAM and NDAM showed cytotoxicity towards OSCC cell lines and the maximum growth inhibition for both compounds was observed in H400 cells with IC 50 value of 1.9 and 6.8 lg/ml, respectively, after 72 h treatment. The results also demonstrated the inhibition of H400 OSCC cells proliferation, internucleosomal cleavage of DNA, activation of intrinsic apoptosis pathway, and cell cycle arrest caused by DAM and NDAM. Therefore, these findings suggest that DAM and NDAM can be potentially used as antitumor agents for oral cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia

et al. 2016

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“…Although many of its compounds are chemically known, the properties of most of these compounds remain unknown. Some pharmacological activities of P. amarus have already been confirmed by scientific studies, such as: (1) treatment for hepatitis B, by suppression of the replication of the virus (Thyagarajan et al, 1988), (2) hepatoprotective effect against actions of paracetamol, carbon tetrachloride (CCl4), galactosamine and alcohol (Krithika et al, 2009, Pramyothin et al, 2007) -phyllanthin and hypophyllanthin, which are lignans present in P. amarus, are described as having hepatocyte-protective action against carbon tetrachloride (Khatoon et al, 2006), (3) aqueous extracts from P. amarus have shown a strong inhibitory action against hepatic carcinoma (Rajeshkumar & Kuttan, 2000), (4) possible antispasmodic effects of the extract on the smooth musculature have been reported because they contribute to the effects on the renal calculi (Kassuya et al, 2003), (5) antibacterial activity of the ethanolic extract (Kloucek et al, 2005), (6) potential anti-inflammatory activity of both the hexane extract and the chemical fraction rich in lignans, obtained from the leaves of P. amarus. The anti-inflammatory activity of lignans was evaluated and it was noticed that nirantin is the most active substance of this fraction.…”

Section: Phyllanthus Amarus Lmentioning

confidence: 94%

The Use of Brazilian Medicinal Plants to Combat Schistosoma mansoni

Allegretti¹,

Oliveira²,

Oliveira³

et al. 2012

Schistosomiasis

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“…Also HepG2 is a popular and an effective in vitro model for assessing hepatoprotective potential of phyto compounds or bioassay guided fractions due to its functional similarity with an intact liver [21] .…”

Section: Cytotoxicity Assessment Of F Limonia Extracts/fractions Andmentioning

confidence: 99%

Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

Jain

Kapadia

Jadeja

et al. 2011

Asian Pacific Journal of Tropical Biomedicine

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Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line

Cited by 108 publications

References 40 publications

Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia

Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia

The Use of Brazilian Medicinal Plants to Combat Schistosoma mansoni

Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

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