Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membrane

Marcus, Akiva; Coyne, Thomas; Rauch, Judah; Woodbury, Dale; Black, Ira B.

doi:10.1111/j.1432-0436.2007.00194.x

Cited by 86 publications

(85 citation statements)

References 63 publications

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“…It is a well-known fact that mixed population of cells cultured from amniotic membrane in many species such as human, ovine, chicken, rat, equine and canine also displayed heterogeneous cell population (Marcus et al 2008;Mauro et al 2010;Bacenkova et al 2011;Coli et al 2011;Gao et al 2012;Park et al 2012). In agreement with these reports, it was observed that cultured cells exhibited both spindleshaped fibroblasts and polygonal shaped epithelial cell-like morphology in vitro.…”

Section: Discussionsupporting

confidence: 80%

“…These results indicate that these cells were in an undifferentiated state. Expression of these pluripotency marker genes has previously been reported in amnion-derived cells of various mammalian species including rat (Marcus et al 2008), canine (Park et al 2012) and chicken (Gao et al 2012). In addition, reverse transcriptase PCR results indicated that AMSCs expressed the mesenchymal stem cells markers CD44 and CD29, but not haematopoietic surface markers such as CD34 as reported earlier in bovine .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Yamahara et al (2014) reported that mesenchymal stem cells isolated from human amniotic tissue have an angiogenesis potential. In animals, amnion-derived stem cells have potential of differentiation into multilineage mesenchymal cell types in many species such as bovine , canine (Park et al 2012), feline (Rutigliano et al 2013), chicken (Gao et al 2012), rat (Marcus et al 2008) and equine (Coli et al 2011;Violini et al 2012;Seo et al 2013). Further, the amnion derived MSCs are reported to express certain key pluripotency markers including OCT4 .…”

Section: Introductionmentioning

confidence: 99%

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Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Shah

Yadav

2015

Cytotechnology

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The developmental ability and gene expression pattern at 8-to 16-cell and blastocyst stages of buffalo (Bubalus bubalis) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical ''S'' shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (OCT4, SOX2, NANOG), and mesenchymal stem cell markers (CD29, CD44) and were negative for haematopoietic marker (CD34) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3-4 passage were employed for NT. The cleavage rate was significantly (P \ 0.05) higher in IVF than in FF-NT and AMSC-NT embryos (82.6 ± 8.2 vs. 64.6 ± 1.3 and 72.3 ± 2.2 %, respectively). However, blastocyst rates in IVF and AMSC-NT embryos (30.6 ± 2.7 and 28.9 ± 3.1 %) did not differ and were significantly (P \ 0.05) higher than FF-NT (19.5 ± 1.8 %). Total cell number did not show significant (P [ 0.05) differences between IVF and AMSC-NT embryos (186.7 ± 4.2, 171.2 ± 3.8, respectively) but were significantly (P \ 0.05) higher than that from FF-NT (151.3 ± 4.1). Alterations in the expression pattern of genes implicated in transcription and pluripotency (OCT4, STAT3, NANOG), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), growth factor signaling and imprinting (IGF2, IGF2R), apoptosis (BAX, BCL2), metabolism (GLUT1) and oxidative stress (MnSOD) regulation were observed in cloned embryos. The transcripts or expression patterns in AMSC-NT embryos more closely followed that of the in vitro derived embryos compared with FF-NT embryos. The results demonstrate that multipotent amnion MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned buffalo embryos.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Shah

Yadav

2015

Cytotechnology

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show abstract

“…It has been reported that rat and human mesenchymal stromal cells can be isolated from amnion (Ilancheran et al 2009). These cell populations display a fibroblast like appearance, adhere onto plastic culture vessels, form clonal colonies (Kim et al 2007) and can differentiate into mature cell lineages in vitro with ectodermal and endodermal characteristics (Marcus et al 2008;Parolini et al 2008), thus resulting a potential source for regenerative medicine applications.…”

Section: Introductionmentioning

confidence: 99%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

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“…2), which is in line with the internationally recognized criteria for MSCs [27,28]. Real-time qPCR showed that PDB-MSCs express the embryonic stem cell (ESC) transcriptional factors Nanog and OCT4.…”

Section: Flow Cytometry Analyses Of Pdb-mscsmentioning

confidence: 62%

miR-375 induces human decidua basalis-derived stromal cells to become insulin-producing cells

Shaer¹,

Azarpira²,

Vahdati³

et al. 2014

Cellular and Molecular Biology Letters

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This paper focuses on the development of renewable sources of isletreplacement tissue for the treatment of type I diabetes mellitus. Placental tissue-derived mesenchymal stem cells (MSCs) are a promising source for regenerative medicine due to their plasticity and easy availability. They have the potential to differentiate into insulin-producing cells. miR-375 is a micro RNA that is expressed in the pancreas and involved in islet development. Human placental decidua basalis MSCs (PDB-MSCs) were cultured from full-term human placenta. The immunophenotype of the isolated cells was checked for CD90, CD105, CD44, CD133 and CD34 markers. The MSCs (P3) were chemically transfected with hsa-miR-375. Total RNA was extracted 4 and 6 days after transfection. The expressions of insulin, NGN3, GLUT2, PAX4, PAX6, KIR6.2, NKX6.1, PDX1, and glucagon genes were evaluated using real-time qPCR. On day 6, we tested the potency of the clusters in response to the high glucose challenge and assessed the presence of insulin and NGN3 proteins via immunocytochemistry. Flow cytometry analysis confirmed that more than 90% of the cells were positive for CD90, CD105 and CD44 and negative for CD133 and CD34. Morphological changes were followed from day 2. Cell clusters formed during day 6. Insulin-producing clusters showed a deep red color with DTZ. The expression of pancreatic-specific transcription factors increased remarkably during the four days after transfection and significantly increased on day 7. The clusters were positive for insulin and NGN3 proteins, and C-peptide and insulin secretion increased in response to changes in the glucose concentration (2.8 mM and 16.7 mM). In conclusion, the MSCs could be programmed into functional insulin-producing cells by transfection of miR-375.

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Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membrane

Cited by 86 publications

References 63 publications

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Isolation and differentiation potential of an equine amnion-derived stromal cell line

miR-375 induces human decidua basalis-derived stromal cells to become insulin-producing cells

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