Isolation, characterization and immunomodulatory activity of oligosaccharides from Codonopsis pilosula

Bai, Ruibin; Zhang, Yajie; Jia, Xusen; Fan, Jingmin; Hou, Xiaohui; Wang, Yanping; Li, Xue; Han, Jiabing; Hu, Fangdi

doi:10.1016/j.jff.2020.104070

Cited by 32 publications

(17 citation statements)

References 36 publications

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“…13 Modern pharmacological studies have confirmed that CPN had good activities in anti-cancer, improving immunity, regulating blood lipid metabolism, protecting liver, kidney and stomach, and protecting neurons. 15–22 Modern phytochemical investigations demonstrated that steroids, terpenoids, polyacetylenes, alkaloids, phenylpropanoids, lignanoids, flavonoids and polysaccharides were the main components of CPN. 14 There are few studies on anti-hyperglycemic activities and active ingredients of CPN.…”

Section: Discussionmentioning

confidence: 99%

Hypoglycemic activity of Codonopsis pilosula (Franch.) Nannf. in vitro and in vivo and its chemical composition identification by UPLC-Triple-TOF-MS/MS

Jia

Jiang

et al. 2022

Food Funct.

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Section: Discussionmentioning

confidence: 99%

Hypoglycemic activity of Codonopsis pilosula (Franch.) Nannf. in vitro and in vivo and its chemical composition identification by UPLC-Triple-TOF-MS/MS

Jia

Jiang

et al. 2022

Food Funct.

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“…The preparation was based on our method reported previously [19,27]. Briefly, after being degreased thrice (1 h/time) with 95% ethanol (1:10, w/v), the dried residue powder (15 g) was decocted and extracted with 10 volumes of water (45 min × 3 times).…”

Section: Preparation Of Monosaccharides Oligosaccharides and Polysacc...mentioning

confidence: 99%

“…For polysaccharides pluralistic analysis, crude polysaccharides samples were made into a 6-mg/mL solution with distilled water and then filtered through a 0.45-μm membrane to determine relative average Mw and distribution by high-performance gel permeation chromatography-ELSD, then monosaccharide composition and proportion were analyzed according to our existing methods [15,27] by GC (Shimadzu, Japan). Finally, the functional groups of polysaccharides were characterized by infrared chromatograph (IR) Vertex 70v spectrophotometer (Bruker Optics, Germany) at a wavenumber of 400-4000 cm −1 .…”

Section: 24mentioning

confidence: 99%

Characterization of chemical composition variations in raw and processed Codonopsis Radix by integrating metabolomics and glycomics based on multiple chromatography‐mass spectrometry technology

Wang

Zhang

Wang

et al. 2022

J of Separation Science

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Codonopsis Radix, a popular food homology medicine, is widely used in clinical traditional Chinese medicine and food supplement, raw products and three types of processed products are the main forms of decoction pieces in China. However, there is no scientific basis for comprehensive chemical characterization of raw and three types of processed products. Herein, we investigated qualitatively and quantificationally secondary and primary metabolites in raw Codonopsis Radix and three types of processed products by metabolomics and glycomics employing multiple chromatography-mass spectrometry technology combined with chemometric analysis further to look for differential compounds and propose the processing-induced chemical mechanisms. The results indicated that Codonopsis Radix became dark-colored and the smell of burnt incense odor was observed after processing. The principal component analysis demonstrated that secondary metabolome and glycome were significantly altered between raw and processed products, and 36 differential secondary metabolites and 11 differential primary metabolites were finally screened through orthogonal partial leastsquares-discriminant analysis. The main types of compounds are alkaloids, terpenoids, glycosides, amino acids, monosaccharides, oligosaccharides, and furfural derivatives. Meanwhile, Chemical mechanisms could be involved, including oxidation, glycosidic hydrolysis, esterification, dehydration, and Maillard reaction. This work supplies a chemical basis for the application of various types of Codonopsis Radix decoction pieces.

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“…by Prof. Xicang Yang from the Affiliated Hospital of the Gansu University of Chinese Medicine. The main components of CR are polysaccharides (40-50%) and oligosaccharides (10-20%), and their extraction and component analysis have been reported previously (Chunxia et al, 2013;Bai et al, 2020). Vitacoenzyme was purchased from Deshengtang Pharmacy (Lanzhou, China).…”

Section: Materials and Reagentsmentioning

confidence: 99%

Proteomics and Metabolomics Unveil Codonopsis pilosula (Franch.) Nannf. Ameliorates Gastric Precancerous Lesions via Regulating Energy Metabolism

Jin

et al. 2022

Front. Pharmacol.

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Objective: This study aimed to systematically evaluate the efficacy of Codonopsis pilosula (Franch.) Nannf. (Codonopsis Radix, CR) and reveal the mechanism of its effects on suppressing Gastric Precancerous Lesions.Methods: First, we established the GPL rat model which was induced by N-methyl-N′-nitro-N-nitrosoguanidine, a disordered diet, and 40% ethanol. The CR’s anti-Gastric Precancerous Lesions effect was comprehensively evaluated by body weight, pathological section, and serum biochemical indexes. Then, quantitative proteomics and metabolomics were conducted to unveil the disturbed protein-network and pharmacodynamic mechanism. Furthermore, serum pharmacology was employed to confirm that CR’s anti-gastritis and anti-cancer phenotype in cell models.Results: In animal models, CR had been shown to control inflammation and ameliorate Gastric Precancerous Lesions. Considering the combination of proteomics and metabolomics, we found that CR could significantly reverse the biological pathways related to energy metabolism which were disturbed by the Gastric Precancerous Lesions model. Furthermore, the results of serum pharmacology indicated that the Codonopsis Radix containing serum could ameliorate gastritis injury and selectively inhibit the proliferation of gastric cancer cells rather than normal cells, which was closely related to ATP production in the above mentioned cells.Conclusion: In summary, CR exerted anti-Gastric Precancerous Lesions effects by ameliorating gastritis injury and selectively inhibiting the proliferation of gastric cancer cells rather than normal cells. Proteomics and metabolomics unveiled that its efficacy was closely related to its regulation of the energy-metabolism pathway. This research not only provided new ideas for exploring the mechanism of complex systems such as Chinese herbals but also benefited the treatment strategy of Gastric Precancerous Lesions via regulating energy metabolism.

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Isolation, characterization and immunomodulatory activity of oligosaccharides from Codonopsis pilosula

Cited by 32 publications

References 36 publications

Hypoglycemic activity of Codonopsis pilosula (Franch.) Nannf. in vitro and in vivo and its chemical composition identification by UPLC-Triple-TOF-MS/MS

Hypoglycemic activity of Codonopsis pilosula (Franch.) Nannf. in vitro and in vivo and its chemical composition identification by UPLC-Triple-TOF-MS/MS

Characterization of chemical composition variations in raw and processed Codonopsis Radix by integrating metabolomics and glycomics based on multiple chromatography‐mass spectrometry technology

Proteomics and Metabolomics Unveil Codonopsis pilosula (Franch.) Nannf. Ameliorates Gastric Precancerous Lesions via Regulating Energy Metabolism

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