Isolation, characterization and molecular three-dimensional structural predictions of metalloprotease from a phytopathogenic fungus, Alternaria solani (Ell. and Mart.) Sor.

Chandrasekaran, M.; Chandrasekar, Raman; Chun, Sechul; Sathiyabama, Muthukrishnan

doi:10.1016/j.jbiosc.2015.12.021

Cited by 11 publications

(4 citation statements)

References 51 publications

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“…Extracellular proteases are key enzymes produced by fungi during the host-pathogen interaction and are involved in growth, development, survival and pathogenicity [ 31 ]. The presence of an extracellular serine protease and metalloprotease was recently found to be secreted by A. solani and proposed to be involved in phytopathogenicity [ 32 , 33 ]. However, additional molecular studies are needed to demonstrate that they play a role in virulence.…”

Section: Early Blightmentioning

confidence: 99%

Current Status of Early Blight Resistance in Tomato: An Update

Adhikari

Panthee

2017

IJMS

171

102

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Early blight (EB) is one of the dreadful diseases of tomato caused by several species of Alternaria including Alternaria linariae (which includes A. solani and A. tomatophila), as well as A. alternata. In some instances, annual economic yield losses due to EB have been estimated at 79%. Alternaria are known only to reproduce asexually, but a highly-virulent isolate has the potential to overcome existing resistance genes. Currently, cultural practices and fungicide applications are employed for the management of EB due to the lack of strong resistant cultivars. Resistance sources have been identified in wild species of tomato; some breeding lines and cultivars with moderate resistance have been developed through conventional breeding methods. Polygenic inheritance of EB resistance, insufficient resistance in cultivated species and the association of EB resistance with undesirable horticultural traits have thwarted the effective breeding of EB resistance in tomato. Several quantitative trait loci (QTL) conferring EB resistance have been detected in the populations derived from different wild species including Solanum habrochaites, Solanum arcanum and S. pimpinellifolium, but none of them could be used in EB resistance breeding due to low individual QTL effects. Pyramiding of those QTLs would provide strong resistance. More research is needed to identify additional sources of useful resistance, to incorporate resistant QTLs into breeding lines through marker-assisted selection (MAS) and to develop resistant cultivars with desirable horticultural traits including high yielding potential and early maturity. This paper will review the current understanding of causal agents of EB of tomato, resistance genetics and breeding, problems associated with breeding and future prospects.

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Section: Early Blightmentioning

confidence: 99%

Current Status of Early Blight Resistance in Tomato: An Update

Adhikari

Panthee

2017

IJMS

171

102

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“…Its optimal temperature was 105°C, much higher than the temperature optimum of purified O. radicata metalloprotease (50°C). The pH and temperature optimum of a metalloprotease from Alternaria solani (Chandrasekaran et al, 2016) exhibited similiarity to the purified O. radicata metalloprotease. When compared with other metalloproteases, the O. radicata metalloprotease showed several notable differences in characteristics.…”

Section: Discussionmentioning

confidence: 90%

Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata

Geng

Gao

et al. 2017

Acta Biochim Pol

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In this study, a 39-kDa metalloprotease was purified from a rare edible mushroom with health-promoting activities, Oudemansiella radicata, using a purification protocol which entailed anion exchange chromatography on DEAE-cellulose and Q-Sepharose columns and gel filtration by fast protein liquid chromatography on a Superdex 75 column. Some peptide sequences were obtained by LC-MS/MS analysis and one of the sequences, DAWIQADVNR, manifested 90% identity to Coprinopsis cinerea metalloprotease. The optimal reaction pH and temperature for Oudemansiella radicata protease were pH 7.0 and 50°C, respectively. The protease was purified 79-fold and demonstrated a specific protease activity of 2.42 U/mg. The K of the purified protease for the casein substrate was 0.65 mg/mL at pH 7.0 and 50°C. The activity of the protease was inhibited by Cd, Hg, Cu, Pb and Fe ions, but was enhanced by K, Mn and Fe ions. The marked suppression of the protease activity by EDTA indicates that the protease is a metalloprotease.

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“…привлекают пристальное внимание как сильные аллергены, критичные на ранних стадиях развития астмы [28,29]. Идентифицирована и охарактеризована щелочная сериновая пептидаза фитопатогенного гриба A. solani и подобраны оптимальные условия ее образования [30,31]. Максимум образования пептидазы в среде, содержащей 1% казеина, был отмечен на 9-е сут.…”

Section: пептидазы микромицетов рода Alternariaunclassified

Fungal Proteolytic Enzymes and Their Inhibitors as Perspective Biocides with Antifungal Action

Shamraychuk

Belyakova

Еремина³

et al. 2020

Moscow Univ. Biol.Sci. Bull.

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Обзор посвящен выявлению групп пептидаз, вовлеченных в процессы роста грибов, а также ингибиторов таких пептидаз. Рассмотрены имеющиеся данные по разнообразию, значимости, представленности и особенностям протеолитических ферментов представителей родов Aspergillus, Penicillium, Trichoderma и Alternaria. На основе анализа данных сделан вывод, что сериновые, металло-и глутаминовые пептидазы необходимы для роста грибов. Поэтому эти ферменты рассматриваются как перспективные мишени ингибиторов пептидаз, что может служить обоснованием поиска таких ингибиторов и разработки на их основе новых биоцидных средств для защиты объектов искусства от биодеструкторов.

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Isolation, characterization and molecular three-dimensional structural predictions of metalloprotease from a phytopathogenic fungus, Alternaria solani (Ell. and Mart.) Sor.

Cited by 11 publications

References 51 publications

Current Status of Early Blight Resistance in Tomato: An Update

Current Status of Early Blight Resistance in Tomato: An Update

Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata

Fungal Proteolytic Enzymes and Their Inhibitors as Perspective Biocides with Antifungal Action

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