Isolation, Characterization and Production of Phytase from Endophytic Fungus its Application for Feed

Marlida, Yetti; Delfita, Rina; Adnadi, Peri; Ciptaan, Gita

doi:10.3923/pjn.2010.471.474

Cited by 36 publications

(26 citation statements)

References 9 publications

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“…Phytase activity for isolates IG 1 and IG 3 was optimum at pH 5.5 as depicted from Figure 1a and optimum temperature was 37 °C and 40 °C respectively as shown in Figure 1b. Phytase often has a low-pH optimum range (pH 4.5–6.0) with a rapid drop in activity at pH value above 6.0 (Marlida et al , 2010). It is needed to be determined that phytase activity for the fungal isolates is extracellular or cell-associated.…”

Section: Resultsmentioning

confidence: 99%

Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis

Gontia‐Mishra¹,

Deshmukh²,

Tripathi³

et al. 2013

Braz. J. Microbiol.

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Phytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work efforts were made to isolate and characterize proficient phytase producing fungi from soil. Phytase producing fungi were isolated using phytate specific medium. Fungal isolates were selected according to their higher phytase activities. These isolates were further characterized and identified by morphological and microscopic analysis and confirmed by amplification of 18S rRNA gene, using specific primers. This gene was subsequently sequenced and phylogenetic affiliations were assigned. Fungal isolates were identified as various species of Aspergillus. Phytases from these fungi could be utilized as a feed additive in poultry and swine industries.

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Section: Resultsmentioning

confidence: 99%

Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis

Gontia‐Mishra¹,

Deshmukh²,

Tripathi³

et al. 2013

Braz. J. Microbiol.

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“…Recently, phytase activity was reported from endophytic fungus Rhizoctonia sp. and Fusarium verticillioides, isolated from leaf, stem and root fragments of soybean (Marlida et al 2010). Similarly, phytase activity is reported for F. oxysporum, a soil isolate (Betancur et al 2012).…”

Section: Introductionmentioning

confidence: 69%

“…Physicochemical property analysis (computed pI) of FoPhy also predicted that this enzyme functions best in acidic environment. Phytase often has a low-pH optimum range (pH = 4.5-6.0) with a rapid drop in activity at pH value above 6.0 (Marlida et al 2010). Thus, the in silico prediction about the function of FoPhy was experimentally validated under the wet-lab conditions.…”

Section: Discussionmentioning

confidence: 99%

Computational identification, homology modelling and docking analysis of phytase protein from Fusarium oxysporum

et al. 2014

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Abstract:The extracellular phytase structural gene was isolated from phytopathogenic fungus Fusarium oxysporum using PCR amplification (GenBank accession number KC708486). The gene possesses an open reading frame of 1,514 bp and two coding regions 1-44 and 156-1458 with one intron (45-155). The phy gene from F. oxysporum (Fophy) encodes a putative phytase protein of F. oxysporum (FoPhy) of 448 amino acids, which includes a putative signal peptide (21 residues). The deduced amino acid sequence of FoPhy exhibits 98% sequence identity with Aspergillus niger and Aspergillus awamori phytases. The deduced protein sequence contains the consensus motifs (RHGXRXP and HD), eight conserved cysteine residues and ten conserved putative N-glycosylation sites, which are conserved among histidine acid phosphatases. Computed structural model of FoPhy was found to consist of mixed α/β motifs and probable loops. The predicted model resembles the structure of Aspergillus niger phytase (root mean square deviation 0.23Å). Ramachandran plot analysis revealed that 95.0% portion of residues fall into the most favourable regions. The predicted three-dimensional structures of FoPhy on molecular docking with substrates like inositol hexaphosphate, inositol hexasulphate and N-acetyl D-glucosamine showed its interaction with conserved histidine and aspartic acid residues in the active site, as also known for other fungal phytases. This study provides a detailed identification and characterization of the phytase from F. oxysporum, which may be helpful in elucidation of its role in pathogenesis and other transcriptional and expression studies.

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“…In this study, G. Klebahnii was observed to produce phytase ( Figure 2a) and inulinase ( Figure 2b).Several fungi such as F. verticillioides, Aspergillus sp., Penicillium sp. and Emericella sp., have been r eported to be phytase (Marlida et al, 2010). Mughal et al (2009) reported 45.65 IU/mL inulinase production from G. candidum.…”

Section: Enzyme Based Screeningmentioning

confidence: 99%

Probiotic attributes of a yeast-like fungus, Geotrichum klebahnii

Syal¹,

Vohra²

2014

Afr. J. Microbiol. Res.

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Geotrichum klebahnii, a filamentous yeast-like fungus, was isolated from a cheese sample. Several in vitro tests were carried out for its probiotic characterization. This isolate showed high survival rate of 100 ± 1.8% at low pH (pH 2) and 100 ± 0.9% at high oxbile concentration (1%) and also grew well at 37°C. It showed an auto-aggregation ability of 100.00 ± 1.5% after 20 h of incubation at 37°C, as well as 36.43 ± 0.70% and 52.13 ± 1.50% cell surface hydrophobicity with xylene and n-hexadecane, respectively. It had inhibitory activity against food-borne pathogens such as Salmonella sp., Vibrio sp. and Staphylococcus aureus. G. klebahnii produced the enzymes phytase and inulinase. It was a producer of vitamin B 12 and exoploysaccharides. It assimilated up to 29.42 ± 2.1% cholesterol after 48 h of incubation at 37°C. The organism did not produce gelatinase and DNase assay, indicating its safety as a probiotic microorganism. This is the first report of the probiotic potential of yeast like fungus, G. klebahnii.

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Isolation, Characterization and Production of Phytase from Endophytic Fungus its Application for Feed

Cited by 36 publications

References 9 publications

Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis

Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis

Computational identification, homology modelling and docking analysis of phytase protein from Fusarium oxysporum

Probiotic attributes of a yeast-like fungus, Geotrichum klebahnii

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