Isolation, Characterization, and Production of Red Pigment from Cercospora piaropi a Biocontrol Agent for Waterhyacinth

Abstract

A red pigment produced by a Mexican isolate of Cercospora piaropi (waterhyacinth pathogen) has been isolated and identified as cercosporin. The kinetic of cercosporin production in culture media during dark/light regimes was evaluated. When C. piaropi was cultivated in continuous light and potato dextrose broth culture, a maximum of cercosporin production was observed (72.59 mg/l). Despite other reports, C piaropi Mexican isolate produce cercosporin in dark conditions (25.70 mg/l). The results suggest that production of cercosporin in C. piaropi-waterhyacinth pathogenesis is an important factor to take into account in biocontrol strategies.

Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

“…1 e, f), for 11 days under continuous light illumination. The total amount of CP was 6.19-fold and 3.65-fold higher than the previously reported condition [ 26 ], and our original condition [ 35 ], respectively. For the Cercospora sp.…”

“…[ 22 , 25 – 27 ]. So far, many previous studies have focused on increasing the productivity of CP by optimizing numerous fermentation factors, including medium, salts, buffers and ions, and delivered the maximum production of 75.59 mg/L after 31 days culture of a Cercospora strain from symptomatic leaves of water hyacinth [ 26 ]. However, both of solid-state and liquid fermentation are currently not able to produce stable and high-yield of CP in a large scale within a reasonable culture time.…”

“…Recently, we identified a new CP-producing strain Cercospora sp. JNU001, which was isolated from bark of Taxus chinensis , with the ability to produce CP at the yield of 128.2 mg/L when it was cultivated under continuous light illumination with S-7 medium after 11 days [ 22 ], which is substantially higher than other previous studies, even within a shortened culture time [ 26 ]. Therefore, herein we attempted to improve the production of CP using Cercospora sp.…”

Antiproliferative Activity of epi-Cercosporin in Human Solid Tumor Cell Lines

“…Therefore, each of these 1 H NMR absorptions corresponds to two equivalent protons, due to the symmetry of the molecule. Similar to (+) cercosporin (1) [1], the 1 H NMR spectrum shows three outstanding signals at  7.03, 5.71 and 4.20 that corresponds to the two aromatic protons (H-5 and H-8), one methylendioxy group at position C-16, and two methoxy groups on C-17 and C-17´ respectively. However, at  3.68, 3 primary carbons ( 61.0 and 24.1).…”

“…It is well known that several of Cercospora species produce a red pigment known as (+) cercosporin (1) [1][2][3]. This deep red compound is a photodynamic pigment [4,5] that was first isolated from a mycelial grown of pathogenic fungus Cercospora kikuchii [6].…”