Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients

Abstract

Introduction:The most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of therapy in model cell of breast cancer.Aim:This study aim to develop an isolation technique of breast cancer cell from patients as a cancer cell model.Material and methods:Breast cancer cell isolation is performed by enzymatic methods using the collagen I and hyaluronidase. Then, breast cancer cells were characterized using flow cytometry based on the CD44/CD24 expression where MDA-MB468 and MCF-7 breast cancer cell lines were used as positive controls. Estrogen receptor (ER), progesterone receptor (PR), p53, HER2, and Ki67 expression were assessed using an immunohistochemistry assay.Result and Discussion:The morphology of cancer cells was fibroblast like cells on the day 7th after isolation. Isolated breast cancer cells expressed 95.33±0.47% of CD44+/CD24+ and human epidermal growth factor receptor 2 (HER2) low expressions. Isolation of breast cancer cells can use In-house enzymatic protocol. Isolated breast cancer showed the same expression as MDA-MB468 (CD44+/CD24+) and HER2- compared to MCF-7 cell lines (CD44-/CD24+).Conclusion:These cells belonged to a basal type of breast carcinoma and expressed CD44+/CD24+, then isolated BCCs can be used as model cancer cells for further research.

Isolation and characterization of the first Slovenian human triple‐negative breast cancer cell line

“…In the last years, several isolation protocols of breast (cancer) tissue cells have been developed and reported . These differ in complexity as well as repeatability.…”

“…In the last years, several isolation protocols of breast (cancer) tissue cells have been developed and reported. 1,2 These differ in complexity as well as repeatability. In this work, we describe a new simple, efficient, and reproducible protocol for isolation and culturing of breast cancer cells from triple-negative breast cancer (TNBC) tissue.…”

Assessment of Intratumoral Heterogeneity in Isolated Human Primary High-Grade Glioma: Cluster of Differentiation 133 and Cluster of Differentiation 15 Double Staining of Glioblastoma Subpopulations

“…+ has capability for unlimited self-renewal and has good ability to initiate and drive brain tumor in vivo [9] and CD133 positivity also has high correlation with survival and chemoresistance in gliomas [18]. However, one study also found that there are CD133-negative (CD133 − ) CSCs in gliomas that exhibit similar property as CD133 + CSCs [19].…”

“…For IDH-1, R132H mutant if stained was negative or < 10% categorized as negative and if > 10% as positive. PD-L1 IHC results were defined on the tumor proportion score as follows based on distributions as 0 (negative), 1 (<20%), 2 (20-50%), 3 (50-80%), 4 (>80%); score for intensity as 0 (negative), 1 (weak), 2 (moderate), and 3 (strong); results from distribution x intensity as histoscore low (0-4), moderate (4-6), and high (6)(7)(8)(9)(10)(11)(12).…”

“…The pellet was then resuspended with the complete growth medium. The cell suspension was next incubated at 37°C, 5% CO 2 [9]. Glioma cells at passage 3, CGNH-89, and U87 cell line (ATCC ® HTB-14 ™ ) were characterized using flow cytometry (Macsquant Analyzer 10) for positive and negative markers of glioblastoma cells at density 1 × 10 5 cells/tube.…”