Isolation, cloning and characterization of two major satellite DNA families of rabbit (Oryctolagus cuniculus)

Ékes, Csaba; Csonka, Erika; Hadlaczky, Gyula; Cserpán, Imre

doi:10.1016/j.gene.2004.09.029

Cited by 11 publications

(15 citation statements)

References 29 publications

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“…Three different families of human satellite sequences are indeed found in pericentromeres of different chromosomes (Miller & Therman 2000), whereas mouse major satellites are quite conserved (Vissel & Choo 1989). In rabbit, Ekes et al (2004) identified two families of DNA repeat sequences: Rsat I and Rsat II that do not hybridize to every chromosome but are both localized in the centromeric regions of the chromosomes. It would therefore be interesting to perform fluorescent in situ hybridization with DNA probes directed against Rsat I and Rsat II both to evaluate i) whether these two families occupy distinct nuclear regions in somatic nuclei and ii) whether different chromosomes have similar dynamics or not during nuclear embryonic reorganization.…”

Section: Discussionmentioning

confidence: 99%

Heterochromatin reprogramming in rabbit embryos after fertilization, intra-, and inter-species SCNT correlates with preimplantation development

Yang¹,

Liu²,

Fleurot³

et al. 2013

Reproduction

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To investigate the embryonic genome organization upon fertilization and somatic cell nuclear transfer (SCNT), we tracked HP1b and CENP, two well-characterized protein markers of pericentric and centromeric compartments respectively, in four types of embryos produced by rabbit in vivo fertilization, rabbit parthenogenesis, rabbit-to-rabbit, and bovine-to-rabbit SCNT. In the interphase nuclei of rabbit cultured fibroblasts, centromeres and associated pericentric heterochromatin are usually isolated. Clustering into higher-order chromatin structures, such as the chromocenters seen in mouse and bovine somatic cells, could not be observed in rabbit fibroblasts. After fertilization, centromeres and associated pericentric heterochromatin are quite dispersed in rabbit embryos. The somatic-like organization is progressively established and completed only by the 8/16-cell stage, a stage that corresponds to major embryonic genome activation in this species. In SCNT embryos, pericentric heterochromatin distribution typical for rabbit and bovine somatic cells was incompletely reverted into the 1-cell embryonic form with remnants of heterochromatin clusters in 100% of bovine-to-rabbit embryos. Subsequently, the donor cell nuclear organization was rapidly re-established by the 4-cell stage. Remarkably, the incomplete remodeling of bovine-to-rabbit 1-cell embryos was associated with delayed transcriptional activation compared with rabbit-to-rabbit embryos. Together, the results confirm that pericentric heterochromatin spatio-temporal reorganization is an important step of embryonic genome reprogramming. It also appears that genome reorganization in SCNT embryos is mainly dependent on the nuclear characteristics of the donor cells, not on the recipient cytoplasm.

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Section: Discussionmentioning

confidence: 99%

Heterochromatin reprogramming in rabbit embryos after fertilization, intra-, and inter-species SCNT correlates with preimplantation development

Yang¹,

Liu²,

Fleurot³

et al. 2013

Reproduction

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“…Unlike the mouse, the remodeling of maternal and paternal genomes that occurs during the two first cell cycles after fertilization (Fulka et al 2008) can be distinguished from the EGA at the 8-cell stage, making the rabbit an interesting model. We took advantage of the identification of two families of DNA repeat sequences (named Rsat I and Rsat II) which both localize to pericentromeric regions (Ékes et al 2004). The Rsat I sequence comprises 375-bp-long repeat units while Rsat II is composed of repeat units between 585 and 590 bp (Ékes et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional analysis of nuclear heterochromatin distribution during early development in the rabbit

et al. 2018

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Changes to the spatial organization of specific chromatin domains such as constitutive heterochromatin have been studied extensively in somatic cells. During early embryonic development, drastic epigenetic reprogramming of both the maternal and paternal genomes, followed by chromatin remodeling at the time of embryonic genome activation (EGA), have been observed in the mouse. Very few studies have been performed in other mammalian species (human, bovine, or rabbit) and the data are far from complete. During this work, we studied the three-dimensional organization of pericentromeric regions during the preimplantation period in the rabbit using specific techniques (3D-FISH) and tools (semi-automated image analysis). We observed that the pericentromeric regions (identified with specific probes for Rsat I and Rsat II genomic sequences) changed their shapes (from pearl necklaces to clusters), their nuclear localizations (from central to peripheral), as from the 4-cell stage. This reorganization goes along with histone modification changes and reduced amount of interactions with nucleolar precursor body surface. Altogether, our results suggest that the 4-cell stage may be a crucial window for events necessary before major EGA, which occurs during the 8-cell stage in the rabbit.Electronic supplementary materialThe online version of this article (10.1007/s00412-018-0671-z) contains supplementary material, which is available to authorized users.

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“…According to their organization, two major classes, interspersed and tandem repeats can be distinguished. Satellite DNAs consist of long tandem arrays of more or less well-defined repeat units and except mammal neocentromeres (Kereso et al, 1996;Barry et al, 1999), all higher eukaryotic centromeres examined so far have been found to consist of families of these highly repeated sequences (Tyler-Smith and Willard, 1993;Ekes et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Suiformes orthologous satellite DNAs as a hallmark of Pecari tajacu and Tayassu pecari (Tayassuidae) evolutionary rearrangements

Adega

Chaves

Guedes-Pinto

2008

Micron

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In a broad general way, eukaryotic satellite DNA sequences are characterized by a highly dynamic molecular behavior due to concerted evolution that leads to rapid change between repeat sequences of different species, achieved by amplification of new variants during speciation or by gradual sequence evolution due to the accumulation of nucleotide substitutions. There are, although exceptions for this almost universal rule. We isolated variants from both the Mc1 and Ac2 pig (Sus scrofa, Suidae) satellite DNA families from the genomes of two Tayassuidae members: Pecari tajacu and Tayassu pecari, which have highly derived karyotypes. The presence of these sequences in both families' genomes (Suidae and Tayassuidae) implies their existence in a common ancestor, what confers to the variants the status of orthology and the approximate age of, at least 40 million years. While at the molecular composition level these orthologous sequences are highly homologous, cross-species physical mapping revealed a completely different chromosomal location in Suidae versus Tayassuidae families, most probably, reflecting the high level of divergence and chromosomes evolution pathways after radiation of each family. Detailed comparative analysis of the satellites assignment on the peccary's chromosomes revealed its co-localization with homologous evolutionary breakpoints in both species, suggesting their involvement in the rearrangement events. The complex behavior of the repeats evolution in the pig/peccaries genomes is here clearly illustrated. These sequences are molecularly preserved for a considerable period of time and display slow rates of sequence change, but show a dynamic motion behavior throughout the peccary's genomes that accompanied the great architectonic reorganization of Tayassuidae chromosomes during evolution.

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Isolation, cloning and characterization of two major satellite DNA families of rabbit (Oryctolagus cuniculus)

Cited by 11 publications

References 29 publications

Heterochromatin reprogramming in rabbit embryos after fertilization, intra-, and inter-species SCNT correlates with preimplantation development

Heterochromatin reprogramming in rabbit embryos after fertilization, intra-, and inter-species SCNT correlates with preimplantation development

Three-dimensional analysis of nuclear heterochromatin distribution during early development in the rabbit

Suiformes orthologous satellite DNAs as a hallmark of Pecari tajacu and Tayassu pecari (Tayassuidae) evolutionary rearrangements

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