Isolation, expression, and biochemical characterization: nitrite reductase from <i>Bacillus cereus</i> LJ01

Huang, Yanyan; Liang, Ming-Hua; Zhao, Sheng; Chen, Simin; Liu, Jinsong; Liu, Dongmei; Lu, Yongzhi

doi:10.1039/d0ra06129h

Cited by 9 publications

(3 citation statements)

References 28 publications

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“…John et al (2020), for instance, outlined many members of Bacillus including Bacillus cereus and Bacillus amyloliquefaciens to have the capacity to break down nitrite and ammonia. Similarly, Huang et al (2020) reported different strains of Bacillus (Bacillus cereus LJ01) as having the capacity to degrade nitrite due to its ability to synthesize nitrite reductase, an enzyme responsible for nitrite degradations. Accordingly, Song et al (2011) revealed that Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and screening of indigenous nitrifying bacteria to enhance nutrient recovery in an aquaponics system

Amin

Agustono

Ali

et al. 2023

J World Aquaculture Soc

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The recycling processes of aquaponic waste into fertilizers are highly dependent on the type and activity of nitrifying bacteria in the system. This study aims to find indigenous nitrifiers to enhance nutrient recovery in an aquaponics system. Twelve indigenous nitrifiers consisting of ammonia and nitrite‐degrading bacteria were isolated from a local earthen pond of Nile tilapia (Oreochromis niloticus), and two isolates (AQ4 and AQ12) were selected due to their highest capacity to degrade either ammonia or nitrate respectively. Based on the 16S rRNA gene sequences, these isolates were identified as Marinobacter nauticus (AQ4) with 98.64% similarity, and Bacillus sp. (AQ12) with 99.89% similarity. When these bacterial isolates were applied in aquaponics systems with bacterial enhancement (aquaponicsBE), the nutrient recovery was significantly higher compared to the control (aquaponicsctrl), indicated by a 500 g higher total harvested biomass. With a significance value of p < 0.05, aquaponicsBE gives 17% higher crude fat and protein retention in vegetables while in fish 14% higher crude fat was found. Energy retention in vegetables was 23% higher than in the control. Furthermore, fish grew better in the aquaponicsBE system, with 0.6%BW/day higher specific growth rate, and 0.05 better feed conversion ratio compared to that of fish reared in the control.

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Section: Discussionmentioning

confidence: 99%

Isolation and screening of indigenous nitrifying bacteria to enhance nutrient recovery in an aquaponics system

Amin

Agustono

Ali

et al. 2023

J World Aquaculture Soc

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“…The effects of different nitrite concentrations (0, 100, and 300 mg/L NaNO 2 ) on the growth of L. fermentum RC4 at 8 h were tested by using scanning electron microscopy (SEM) (JSM 6390 LV; JSM, Tokyo, Japan) and surface hydrophobicity (CSH). 7 SEM measurement was operated at 10 kV. Briefly, the pellets of the Control, Nitrite100, and Nitrite300 groups were obtained by centrifugation (8000 rpm, 10 min), washed, and promptly fixed with 2.5% glutaraldehyde, 4 °C for 2 h. Then the cells were washed three times with 0.1 M phosphate buffer saline (PBS, pH 7.2) and dehydrated in a graded ethanol series (30,50,70,80, 95, 100%) each for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Transcriptomic Responses to Nitrite Degradation by Limosilactobacillus fermentum RC4 and Effect of ndh Gene Overexpression on Nitrite Degradation

Shi,

Che,

Sun

et al. 2023

J. Agric. Food Chem.

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The excessive nitrite residue may increase cell damage and cancer risk. Limosilactobacillu fermentum RC4 exhibited excellent nitrite degradation ability. Herein, the molecular mechanism of nitrite degradation by L. fermentum RC4 was studied by integrating scanning electron microscopy analysis, transcriptomics, and gene overexpression. The results demonstrated that the gene profile of RC4 cultured in MRS broth with 0, 100, and 300 mg/L NaNO 2 varied considerably; RC4 responded to nitrite degradation by regulating pyruvate metabolism, energy synthesis, nitrite metabolism, redox equilibrium, protein protection, and signaling. High nitrite concentrations affected the morphology of RC4 with a longer phenotype, rough and wrinkle cell and reduced cell surface hydrophobicity. Moreover, an up-regulated expression of gene ndh encoding NADH dehydrogenase, which provides electrons for nitrite reduction by catalyzing NADH, was identified when RC4 was exposed to nitrite. Overexpression of ndh in RC4 increased the nitrite degradation rate by 2−9.5% in MRS broth with 100 mg/L NaNO 2 . Thus, the findings of this study could be helpful for the application of L. fermentum to reduce nitrite residues and improve food safety in fermented food products.

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Nitrite reductases of lactic acid bacteria: Regulation of enzyme synthesis and activity, and different applications

Yuan,

Zeng,

Zhang

et al. 2024

Food Bioscience

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Isolation, expression, and biochemical characterization: nitrite reductase from Bacillus cereus LJ01

Abstract: Biological remediation of toxic oxygen-containing anions such as nitrite in the environment is of great significance. Bacillus cereus LJ01 showed the activity of degradation for nitrite. the enzyme NiR from LJ01 can degrade the nitrite in vitro.

Cited by 9 publications

References 28 publications

Isolation and screening of indigenous nitrifying bacteria to enhance nutrient recovery in an aquaponics system

Isolation and screening of indigenous nitrifying bacteria to enhance nutrient recovery in an aquaponics system

Transcriptomic Responses to Nitrite Degradation by Limosilactobacillus fermentum RC4 and Effect of ndh Gene Overexpression on Nitrite Degradation

Nitrite reductases of lactic acid bacteria: Regulation of enzyme synthesis and activity, and different applications

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