Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction

Ifeoma, Obidike; Okhale, Samuel; Itohan, Aboh Mercy; Adeola, Salawu Oluwakanyinsola

doi:10.1016/s1995-7645(13)60018-8

Cited by 27 publications

(14 citation statements)

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“…A total of 17 metabolites were identified from UPLCMS/MS, including ellagic acid, gallic acid, quercetin 3-O-rhamnoside, quercetin 3-Oglucoside, catechin, epicatechin, rutin, corilagin, chlorogenic acid, phyllanthin, hypophyllanthin, caffeic acid and malic acid. These metabolites were previously reported in both Phyllanthus species (Chang et al, 2003;Ifeoma et al, 2013;Kumar et al, 2015) and their fragmentation patterns were compared to literature data (Soong and Barlow, 2005;Sudjaroen et al, 2012) as well as co-chromatography with the standards. Phyllanthin and hypophyllanthin were identified and confirmed by co-chromatography with authentic standards, as well as UPLCMS/MS studies and comparison with literature data (Kumar et al, 2015;Somanabandhu et al, 1993).…”

Section: Resultsmentioning

confidence: 93%

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

Mediani

Abas

Khatib

et al. 2015

Industrial Crops and Products

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a b s t r a c tSeveral studies have suggested that plants are potential sources of bioactive compounds. Phyllanthus is a plant genus that has been used in traditional medicine due to its phytomedicinal metabolites content. The variation between two Phyllanthus species (P. niruri and P. urinaria) was studied using proton nuclear magnetic resonance ( 1 H NMR) combined with multivariate data analysis (MVDA). The total phenolic content (TPC), DPPH radical scavenging activity and ␣-glucosidase inhibitory activity of the Phyllanthus species were also evaluated and correlated with their phytochemical constituents at different growth stages (8, 10 and 12 weeks) using partial least square regression (PLS). Principal component analysis (PCA) and PLS indicated separation between the two species based on the identified metabolites and the screened bioactivities. A comparison of the two species indicated that P. urinaria was separated from P. niruri due to its larger quantity of fatty and amino acids, choline, phyllanthin and sucrose. However, P. niruri contained higher quantities of hypophyllanthin and phenolic compounds. The loading column plot, which was used to compare the P. niruri at different growth stages, indicated that the eight-week-old plant contained a higher amount of fatty acids, amino acids (leucine and alanine), phyllanthin and choline. The dominant substances in the P. niruri at 10 weeks of growth by PC1 were identified as hypophyllanthin, malic acid, sucrose, and identified phenolics. The 12 week sample was differentiated by its higher sugars contents as well as malic acid and leucine. The harvested samples of both Phyllanthus species at ten weeks of age exhibited significant bioactivities with the highest content and number of metabolites.

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Section: Resultsmentioning

confidence: 93%

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

Mediani

Abas

Khatib

et al. 2015

Industrial Crops and Products

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“…The crude extract was concentrated under a vacuum and evaporated to dryness to yield a brown, sticky fraction (F1, F2, F3, F4, F5, F6, F7 and F8; 5 g/ml) (5). The crude extract and its fractions were stored in a refrigerator until use.…”

Section: Methodsmentioning

confidence: 99%

A novel assay for high-throughput screening of anti-Alzheimer’s disease drugs to determine their efficacy by real-time monitoring of changes in PC12 cell proliferation

Hou

Yan

Yang

et al. 2013

International Journal of Molecular Medicine

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Alzheimer’s disease (AD) is a neurodegenerative disease that is characterized by the accumulation of senile plaque and neurofibrilary tangle formation in the brain, including the cerebral cortex and hippocampus. Nowadays, the first-line treatment for AD is the application of acetylcholinesterase inhibitors. However, acetylcholinesterase inhibitors are basically anti-symptomatic for a limited aspect of AD pathology and are associated with serious side-effects. With the advantage of multiple targets, pathways and systems, Chinese herbal compounds hold promising potential for the development of drugs for the treatment of AD. Over the past few years, with the development of Chinese herbal compounds and in vitro pharmacological studies, cell-based disease models are one of the main methods used to screen Chinese herbal compounds for potential efficacy. Testing the efficacy of possible anti-Alzheimer’s disease drugs and the development of new drugs are hindered by the lack of objective high-throughput screening methods. Currently, the assessment of the effects of drugs is usually made by MTT assays, involving laborious, subjective, low-throughput methods. Herein, we suggest a novel application for a real-time cell monitoring device (xCELLigence) that can simply and objectively assess the effective composition of Chinese herbal compounds by assessing amyloid-β peptide Aβ1-42-induced apoptosis in PC12 cells. We detected the proliferation and motility of the cells using a fully automated high-throughput and real-time system. We quantitatively assessed cell motility and determined the real-time IC50 values of various anti-AD drugs that intervene in several developmental stages of Aβ1-42-induced apoptosis in PC12 cells, Then, we identified the optimal time phase by curative efficacy. Our data indicate that this technique may aid in the discovery and development of novel anti-Alzheimer’s disease drugs. It is possible to utilize a similar technique to measure changes in electrical impedance as cells attach and spread in a culture dish covered with a gold microelectrode array that covers approximately 80% of the area on the bottom of a well. As cells attach and spread on the electrode surface, it leads to an increase in electrical impedance of 9–12. The impedance is displayed as a dimensionless parameter termed the cell index, which is directly proportional to the total area of tissue culture well that is covered by the cells. Hence, the cell index can be used to monitor cell adhesion, spreading, morphological variation and cell density.

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“…In summary, P. emblica , P. reticulatus , and P. niruri are the top three species widely used around the world. P. niruri is probably the most widespread herb of Phyllanthus , which is named “chanka piedra,” “bhuiamlki,” “zhuzicao,” “dukung anak,” “quebra-pedra,” and “chanca piedra.” Its whole plant can treat inflammation, lithiasis, fever, malaria, hepatitis, and gonorrhea [ 7 , 18 , 19 , 21 , 22 ].…”

Section: Ethnopharmacological Usesmentioning

confidence: 99%

The Genus Phyllanthus: An Ethnopharmacological, Phytochemical, and Pharmacological Review

Mao

Guo

et al. 2016

Evidence-Based Complementary and Alternative Medicine

108

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The plants of the genus Phyllanthus (Euphorbiaceae) have been used as traditional medicinal materials for a long time in China, India, Brazil, and the Southeast Asian countries. They can be used for the treatment of digestive disease, jaundice, and renal calculus. This review discusses the ethnopharmacological, phytochemical, and pharmacological studies of Phyllanthus over the past few decades. More than 510 compounds have been isolated, the majority of which are lignins, triterpenoids, flavonoids, and tannins. The researches of their remarkable antiviral, antioxidant, antidiabetic, and anticancer activities have become hot topics. More pharmacological screenings and phytochemical investigations are required to support the traditional uses and develop leading compounds.

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Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction

Abstract: These findings indicate the antiplasmodial efficacy of P. niruri methanol extract, and the localization of this effect in its chloroform fraction.

Cited by 27 publications

References 27 publications

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

A novel assay for high-throughput screening of anti-Alzheimer’s disease drugs to determine their efficacy by real-time monitoring of changes in PC12 cell proliferation

The Genus Phyllanthus: An Ethnopharmacological, Phytochemical, and Pharmacological Review

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