Isolation Frequency and Antimicrobial Susceptibility of Bacterial Pathogens Isolated from Physical Therapeutic Instruments in General Hospitals

Abstract

Abstract.[Purpose] As demands for physical therapy increase, the numbers of patients in physical therapy rooms are rapidly increasing. However, studies on the state of microorganism infections of physical therapy equipment and their effects on hospital-acquired infections are currently lacking. The purpose of this study was to examine the rate of infections of physical therapy rooms by pathogenic microorganisms.[Methods] Facilities in physical therapy rooms were divided into three groups, therapeutic environments, wet therapeutic equipment, and dry therapeutic equipment, to check the degree of microbial infection and to conduct antimicrobial susceptibility tests on microorganisms that could be isolated.[Results] The main microorganisms isolated from the therapeutic environments were Staphylococcus spp., including Staphylococcus aureus (S. aureus), and 20% of these isolates were methicillin-resistant S. aureus (MRSA). From the wet therapeutic equipment, Acinetobacter spp., including Acinetobacter baumannii (A. baumannii) and A. lowffii, were the primary isolates and 25% of these isolated bacteria were also identified as multi-drug resistant forms. No microorganism was detected from moist hot packs or hot pack units. Diverse bacterial strains were isolated from dry therapeutic equipment, but the isolation rate of Staphylococcus spp. was a little higher than that of other strains.[Conclusion] Our result suggests the possibility of hospital-acquired infection in physical therapy rooms could be very high. Strict hygiene management systems should be established in order to reduce hospital infection during physical therapy.

Magnitude, diversity, and antibiogram of bacteria isolated from the selected wards of Arba Minch hospital, Arba Minch, Ethiopia

“…In the present study, the predominant Gramnegative bacilli, Acinetobacter sp. displayed the highest resistance to two commonly used antibiotics, such as ceftriaxone and ampicillin and are more or less comparable to the results of a prior study [30]. Especially, the isolates of Acinetobacter were found to be susceptible to amikacin.…”

“…Earlier it was reported that Acinetobacter sp. is the main Gram-negative bacilli isolated from therapeutic equipments of hospital settings [28,30]. In contrast, some other studies described that E. coli was the most common Gram-negative bacteria isolated from medical equipments and hospital environment [14,20,23], whereas another study described that Enterobacter sp.…”

High prevalence of Enterococcus spp. from dogs with otitis externa

“…Likewise, most E. faecium isolates were resistant to ampicillin and tetracycline, and were sensitive to imipenem, nitrofurantoin and teicoplanin like those of E. faecalis isolates. E. faecalis and E. faecium isolates showed VanB phenotype which is resistant to vancomycin but sensitive to teicoplanin were 58% and 25%, respectively (Table 2), (Kim et al, 2010b). All of Staphylococcus spp.…”

“…Their genomic DNAs were extracted using an Accuprep genomic DNA extraction kit (Bioneer, Korea). PCR using 16S rRNA primer sets was conducted as described previously (Kim et al, 2010b). After electrophoresis on agarose gels, individual amplified products were purified using an Accuprep gel purification kit (Bioneer, Korea).…”

“…The sequences were identified through BLAST (http://www.ncbi.nlm.nih.gov) analysis. For analysis of van gene typing, PCR using van primer sets was conducted as described previously (Kim et al, 2010b).…”