Isolation frequency and efficiency of mycoparasitic Pythium species in the West Mediterranean region of Turkey

Ağaner, Güliz Tepedelen; Karaca, Gürsel; Khatua, Ashapurna; Paul, Bernard

doi:10.1016/j.crbiot.2021.01.002

Cited by 3 publications

(4 citation statements)

References 29 publications

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“…Previous studies conducted using a similar isolation method (SBT) reported different dominant species, namely P. oligandrum , P. acanthicum , and P. periplocum (Ali‐Shtayeh & Saleh, 1999; Ribeiro & Butler, 1992). In Turkey, the most frequently isolated mycoparasite was P. amasculinum , followed by P. lycopersicum , and P. oligandrum (Ağaner et al, 2021). In the present study, nystatin was used instead of pimaricin, which was utilized as an antifungal antibiotic in the selective media in the studies mentioned above.…”

Section: Discussionmentioning

confidence: 99%

“…Mycoparasitic Pythium s.l. strains were isolated from soils using the sclerotia baiting technique (SBT) described by Ribeiro and Butler (1992), which has been proven to be the most efficient method but is not entirely specific and also isolates non-mycoparasitic species (A ganer et al, 2021;Ali-Shtayeh & Saleh, 1999;Ribeiro & Butler, 1992). Sclerotia of S. sclerotiorum P8 were produced on PDA (39 g/L, Merck, Germany).…”

Section: Isolation Of Mycoparasitic Pythium Sl Isolatesmentioning

confidence: 99%

“…Most well‐known mycoparasitic Pythium s.l. species such as P. acanthicum Drechsler (Deacon & Henry, 1978), P. acanthophoron Sideris (Lodha & Webster, 1990), P. amasculinum Yu (Ağaner et al, 2021), P. lycopersicum Karaca, Tepedelen, Belghouthi & B. Paul (Karaca et al, 2008), P. oligandrum Drechsler (Benhamou et al, 2012), and P. periplocum Drechsler (Deacon & Henry, 1978) are characterized by their spiny oogonia ( Pythium s.l. clade D) and currently belong to Pythium s.s. (Nguyen et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of mycoparasitic Pythium sensu stricto and Globisporangium species in West Azarbaijan province of Iran

Rezaei,

Abrinbana,

Ghosta

2024

Annals of Applied Biology

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Pythium sensu stricto (s.s.) and Globisporangium species are important components of the soil microbial community and exhibit diverse lifestyles, including mycoparasitism. However, a comprehensive understanding of the species diversity of these mycoparasites in the West Azarbaijan province of Iran is lacking. In this study, a total of 114 mycoparasitic Pythium s.s. and Globisporangium isolates were obtained from agricultural soils collected from six regions in the province. Through DNA barcoding, all Globisporangium isolates were identified as G. nunn, while the barcode markers were insufficient to accurately resolve species boundaries in Pythium s.s. By combining morphological and multilocus sequence data, five species within the genus Pythium s.s. were identified: P. salmasense sp. nov., a potentially new species, and three known species, P. acanthicum, P. ornamentatum, and P. periplocum. Pythium ornamentatum was the most common species and found in all regions studied, followed by G. nunn and P. acanthicum, which were both isolated from four regions. While the isolates of G. nunn showed no mycoparasitic activity against Sclerotinia sclerotiorum, all Pythium s.s. species were capable of infecting the hyphae of this pathogen. The existence of mycoparasitic species is promising for biological control of soil‐borne fungal pathogens in the province. The widespread occurrence of P. ornamentatum, G. nunn, and P. acanthicum may suggest their adaptation to local soil and environmental conditions, indicating their potentially superior effectiveness in controlling plant diseases across different regions if used as biocontrol agents.

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Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Mycoparasitic Pythium Sl Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of mycoparasitic Pythium sensu stricto and Globisporangium species in West Azarbaijan province of Iran

Rezaei,

Abrinbana,

Ghosta

2024

Annals of Applied Biology

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“…Mycoparasitic Pythium species are among the biological agents used against different plant pathogens (Paulitz and Baker, 1987;Martin and Hancock, 1987;Lodha and Webster, 1990;Ağaner et al 2021). Pythium oligandrum Drechsler (Deacon, 1976), P. acanthicum Drechsler (Deacon and Henry, 1978), P. nunn Lifshitz, Stanghellini & Baker (Lifshitz et al 1984), P. periplocum Drechsler (Hockenhull et al 1992), P. acanthophoron Sideris and P. mycoparasiticum Deacon, S.A.K.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes

Ateş,

Hatat Karaca

2024

International Journal of Agriculture Environment and Food Sciences

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In this study, antagonistic effects of Pythium acanthophoron, P. lycopersicum, P. oligandrum and P. paroecandrum against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea were investigated by in vitro and in vivo trials. In vitro mycoparasitic activities of Pythium species were determined by dual culture, inverted plate culture and agar diffusion tests. As a result of dual culture tests, suppressive effects of all mycoparasites were over 70% against mycelial growth of the pathogens. Inverted plate tests showed that antagonistic effects of mycoparasites regarding their volatile compounds were rather low. In the agar diffusion test, all mycoparasites showed antibiosis effect, however P. lycopersicum had the highest suppressive effect on both pathogens. In pot trials, mycoparasites were effective to protect tomato seedlings when pathogens were seperately inoculated, and suppressed the symptoms. When two pathogens were inoculated together, P. paroecandrum was ineffective against B. cinerea, but decreased the severity of wilt symptoms, while other mycoparasites totally inhibited both diseases. Chromatographic analyses made by using leaf samples taken 12, 24, 48 and 72 hours after pathogen inoculation showed meaningful increase on chlorogenic acid, caffeic acid and epicatechine, in the samples taken 48 hours after inoculation. Analyses after the inoculations of tomatoes with the mycoparasites and/or pathogens showed that mycoparasites also caused increase in the amounts of phenolics. This indicated that the mycoparasites could be effective to induce defense mechanisms of tomato plants against pathogens. Among them, P. oligandrum can be mentioned as the most effective mycoparasite regarding the induction of phenolics.

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Characterization and Pathogenicity ofPythium-Like Species Associated with Root and Collar Rot of Kiwifruit in Turkey

et al. 2022

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During the period of June to October in 2018, a widespread decline was observed on kiwifruit vines in the vineyards located in Altınordu, Fatsa, and Perşembe districts of Ordu province. The symptoms were associated with reddish-brown rots expanding from the root to the collar with sparse off-color foliage. Based on the percentage of the total infected samples across 18 vineyards, the most common oomycete species were Globisporangium intermedium (37.1%), Phytopythium vexans (34.3%), G. sylvaticum (14.3%), G. heterothallicum (11.4%), and Pythium dissotocum (2.9%). The morphological identification of isolates was confirmed based on partial DNA sequences containing the nuclear rDNA internal transcribed spacer region (rDNA ITS) and the mitochondrial cytochrome c oxidase subunit II (coxII) gene. The optimum growth temperature and the optimum pH of 5 species ranged from 22.98 to 28.25°C and 5.67 to 8.51, respectively. Pathogenicity tests on the seedlings of kiwifruit cv. Hayward revealed significant differences in virulence among isolates. Phytopythium vexans and G. sylvaticum isolates caused severe root and collar rot resulting in seedling death, while G. heterothallicum and G. intermedium isolates had relatively lower virulence. All Globisporangium spp. and P. vexans isolates significantly decreased plant growth parameters (plant height, shoot and root dry weights and root length); however, P. dissotocum caused very mild symptoms and did not affect these parameters of growth. To our knowledge, this is the first study reporting G. sylvaticum, G. heterothallicum, and G. intermedium causing root and collar rot on kiwifruit not only in Turkey but also in the world.

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Isolation frequency and efficiency of mycoparasitic Pythium species in the West Mediterranean region of Turkey

Cited by 3 publications

References 29 publications

Diversity of mycoparasitic Pythium sensu stricto and Globisporangium species in West Azarbaijan province of Iran

Diversity of mycoparasitic Pythium sensu stricto and Globisporangium species in West Azarbaijan province of Iran

Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes

Characterization and Pathogenicity ofPythium-Like Species Associated with Root and Collar Rot of Kiwifruit in Turkey

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