Isolation, identification and antimicrobial resistance profile of Staphylococcus aureus in Cockroaches (Periplaneta Americana)

Abstract

Objective:The study was conducted to determine the prevalence of Staphylococcus aureus in cockroaches (Periplaneta americana), and to assess the antimicrobial resistance profiles of the isolated bacteria. Materials and methods: A total of 150 cockroaches (P. americana) were randomly captured from three households and four restaurants in Chittagong City Corporation, Bangladesh during July to December 2014. The cockroaches were transported to the bacteriology laboratory at the Poultry Research and Training Centre (PRTC), Chittagong Veterinary and Animal Sciences University. The isolation and identification of Staphylococcus spp. from the external surface wash and gut homogenates by pooling cockroaches were done by following conventional bacteriological examinations followed by biochemical characterization. The antibiotic susceptibility profiles of the isolates were determined using disc diffusion method. Results: In this study, the overall prevalence of S. aureus was 38% (n=57/150). Higher prevalence of Staphylococcus spp. was observed among the cockroaches from restaurant (49.3%; n=37/75) as compared to those of households (26.7%; n=20/75) having a significant difference (P<0.05). Highest level of resistance by the Staphylococcus spp. was found to Penicillin (68%) followed by Erythromycin (60%), Oxacillin (46%) and Clindamycin (31%). On the other hand, the Staphylococci isolates were highly sensitive to Cephalothin (84%) and Kanamycin (65%). Conclusion:The rational use of antibiotics needs to be adopted in both human and animal medicine practices to prevent the emergence of drug resistant Staphylococcus spp.

Antibiogram and phylogenetic diversity of enterotoxigenic Staphylococcus aureus strains from milk products and public health implications

“…Moreover, ( Abraha et al, 2017 ) recovered S. aureus isolates from raw milk that were highly susceptible to Gentamicin (100%) in Ethiopia. Furthermore, a study from Bangladesh ( Islam et al, 2016 ) reported the tolerance of S. aureus strains from uncooked milk samples to Tetracycline (73.33%) and susceptibility to Tentamicin (100%). In contrast, ( Akanbi et al, 2017 ) demonstrated high susceptibility to Tetracycline and Sulfamethoxazole-Trimethoprim (56.7%), Ciprofloxacin (66.7%) and Cefoxitin (76.7%).…”

Molecular and Virulence Characteristics of Methicillin-Resistant Staphylococcus aureus Bacteria Recovered From Hospital Cockroaches

“…Consequently, PVL-positive MRSA bacteria recovered from hospital cockroaches may originate from the cases of soft tissue infections in hospitals. Methicillin-resistant S. aureus has been presented as one of the most predominant pathogenic bacteria recovered from B. germanica and P. americana cockroaches (3,4,(21)(22)(23). Nevertheless, the isolation of the SCCmec types and the PVL gene among MRSA bacteria recovered from hospital cockroaches was not scrutinized beforehand.…”

Prevalence and Molecular Detection of Methicillin-Resistant Staphylococcus Aureus From Dogs and Cats in Dhaka City

“…In 1972, MRSA was found in milk from Belgian cows with mastitis. There are reports on the detection of MRSA from dogs, cats, sheep, cattle, horses, rabbits, seals, cockroach, guinea pig and chinchilla (Morgan, 2008;Chandrasekaran et al, 2014;Gulani et al, 2016;Islam et al, 2016). The mecA gene has been detected from Staphylococcus isolated from dairy cattle mastitis (Rahman et al, 2005).…”