Isolation, Identification and Characterization of Bioflocculant-Producing Bacteria from Activated Sludge of Vulindlela Wastewater Treatment Plant

Nkosi, Nkanyiso C.; Basson, Albertus K.; Ntombela, Zuzingcebo G.; Maliehe, Tsolanku Sidney; Pullabhotla, Viswanadha Srirama Rajasekhar

doi:10.3390/applmicrobiol1030038

Cited by 13 publications

(4 citation statements)

References 66 publications

(130 reference statements)

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“…This meant that maximum bioflocculant production occurred during the late exponential stage or early stationary phase of growth. Similar findings have been obtained in other studies [ 59 , 60 ].…”

Section: Resultssupporting

confidence: 93%

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Isolation and Optimisation of Culture Conditions for a Marine Bioflocculant-Producing Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Selepe

Maliehe

Moganedi

et al. 2022

IJERPH

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The application of bioflocculants has become an alternative to that of chemical flocculants in wastewater treatment due to their environmental friendliness and non-toxic effects. This study aimed at isolating a bioflocculant-producing bacterium from marine water, optimisation of its culture conditions, and investigation of the removal efficiency of its bioflocculant on pollutants in wastewater. The bacterium was identified by 16S rRNA gene analysis. Optimal carbon and nitrogen sources, inoculum size, temperature, pH, and time were determined by the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on African green monkey kidney and bovine dermis cells using a tetrazolium-based columetric (MTT) method. Its removal efficiencies on chemical oxygen demand (COD), biological oxygen demand (BOD) and sulphur were determined using the Jar test method. The bacterial isolate was identified as Ochrobactrum oryzae AB84113. A maximum flocculating activity of 92% and a yield of 3.768 g/L were obtained when a 1% (v/v) inoculum size was used in the presence of starch and yeast extract at pH 7, 30 °C, and after 72 h of cultivation. The bioflocculant demonstrated non-cytotoxic effects on bovine dermis and African green monkey kidney cells. The bioflocculant removed 98% COD, 91% BOD and 86% of Sulphur. The bioflocculant has potential for pollutant removal from industrial wastewater.

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Section: Resultssupporting

confidence: 93%

“…When it was heated at 200 °C, the bioflocculant lost 22% of its total weight, and 35% at 450 °C. The weight loss at 200 and 460 °C could be related to degradation of the bioflocculant [ 60 ]. The bioflocculant maintained approximately 62.8% of its weight after being heated at 800 °C.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Optimisation of Culture Conditions for a Marine Bioflocculant-Producing Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Selepe

Maliehe

Moganedi

et al. 2022

IJERPH

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“…The inoculums were then incubated for 72 h at 30 o C at the shaking speed of 160 rpm. Afterwards, the broth culture was centrifuged at 13,000 rpm for 15 min and the cell free supernatant was analysed for flocculating activity (FA) [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Bioflocculation of pollutants in wastewater using flocculant derived from Providencia huaxiensis OR794369.1

Selepe,

Maliehe

2024

BMC Microbiol

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Background Water pollution has become a major environmental and health concern due to increasing population and industrialisation. Microbial flocculants are promising agents for treatment of contaminated water owing to their effectiveness, eco-friendliness, and high biosafety levels. In this study, culture conditions of Providencia huaxiensis OR794369.1 were optimised and its bioflocculant was extracted, characterised and used to treat wastewater. Results The maximum flocculating activity of 92% and yield of 3.5 g/L were obtained when cultivation conditions were: 3% inoculum size, starch, casein, initial pH of 6, cultivation temperature of 30 oC and 72 h of fermentation. The bioflocculant is an amorphous glycoprotein biomolecule with 37.5% carbohydrates, 27.9% protein, and 34.6% uronic acids. It is composed of hydroxyl, amino, alkanes, carboxylic acid and amines groups as its main functional structures. It was found to be safe to use as it demonstrated non-cytotoxic effects on bovine dermis and African green monkey kidney cells, illustrating median inhibitory concentration (IC50) values of 180 and > 500 µg/mL on both cell lines, respectively. It demonstrated the removal efficiencies of 90% on chemical oxygen demand (COD), 97% on biological oxygen demand (BOD) and 72% on Sulphur on coal mine wastewater. It also revealed the reduction efficacies of 98% (COD) and 92% (BOD) and 70% on Sulphur on domestic wastewater. Conclusion The bioflocculant was effective in reducing pollutants and thus, illustrated potential to be used in wastewater treatment process as an alternative.

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“…Colonies with different colour, shape and sizes were selected. Thereafter, the isolates were screened for bioflocculant production by evaluating the flocculating activity of each isolate [25].…”

Section: Isolation Of Target Bacteriamentioning

confidence: 99%

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Maliehe

Moganedi

Masoko

et al. 2022

Microbiology Research

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Bioflocculation has become the method of choice in wastewater treatment because of its effectiveness, environmental friendliness and innocuousness to humans. In this study, the bioflocculant-producing bacterium was isolated and its bioflocculant was used in wastewater treatment. The isolate was identified by 16S rRNA gene sequencing analysis. Its culture conditions (inoculum size, carbon and nitrogen sources, pH, temperature and time) were optimised using the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on human colorectal adenocarcinoma cells (Caco2) by tetrazolium-based colorimetric method. The ability of the bioflocculant to reduce biochemical oxygen demand (BOD) and chemical oxygen demand (COD) in wastewater was evaluated using Jar test. The bacterium was identified as Bacillus subtilis CSM5 and the maximum flocculating activity of 92% was observed when fructose and urea were used as nutrients and the culture conditions were adjusted to 30 °C, pH 9, 160 rpm and 72 h of incubation. Caco2 exhibited 90% viability when the highest bioflocculant concentration of 200 µg/µL was used. The reduction of BOD and COD was achieved at 59 ± 3.1 and 75 ± 0.4%, respectively. In conclusion, B. subtilis CSM5 is a good candidate for bioflocculant production and its bioflocculant has good potential for use in wastewater treatment.

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Isolation, Identification and Characterization of Bioflocculant-Producing Bacteria from Activated Sludge of Vulindlela Wastewater Treatment Plant

Cited by 13 publications

References 66 publications

Isolation and Optimisation of Culture Conditions for a Marine Bioflocculant-Producing Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Isolation and Optimisation of Culture Conditions for a Marine Bioflocculant-Producing Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Bioflocculation of pollutants in wastewater using flocculant derived from Providencia huaxiensis OR794369.1

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

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