Isolation, Identification and Degradation Characteristics of Oil Degrading Bacterial Strain

Zhan, Yabin; Tao, Xingling; He, Shan-Wen; Song, Santai; Xing, Jingjing; Li, Fangmin; Jiang, Tao; Ma, Linna

doi:10.4236/oalib.1104016

Cited by 4 publications

(9 citation statements)

References 15 publications

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“…Our results are consistent with the earlier studies which have reported different species of Burkholderia capable of degrading variable aliphatic and aromatic hydrocarbons of petrol (Marin et al 2001;Chakraborty et al 2010;Mujahid et al 2015). Moreover, petrol hydrocarbon metabolizing Brevibacilli have also been reported in the literature (Xue et al 2006;Mnif et al 2011;Zhan et al 2017).…”

Section: Discussionsupporting

confidence: 93%

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Muccee

Ejaz

2019

Polish Journal of Microbiology

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The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Brevibacillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

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Section: Discussionsupporting

confidence: 93%

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Muccee

Ejaz

2019

Polish Journal of Microbiology

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“…The optimal oil removal condition of G-94 was determined through biomass of petroleum degradation microorganism, not the oil removal rate. The reason why we use the biomass is that biomass is positively correlated with degradation rate and it is more convenient than oil removal rate [10].…”

Section: Discussionmentioning

confidence: 99%

“…Experiments were conducted in the oil medium described above with 0.5% oil (w/v) as sole carbon and energy source. Growth was monitored by measuring optical density at 420 nm with a TU-1900 spectrophotometer [10].…”

Section: Growth Studymentioning

confidence: 99%

“…The oil removal rate was determined by gravimetric method [10]. A total of 80 ml methylene chloride was added to the oil triangle bottle to extract the oil.…”

Section: Determination Of Oil Removal Ratementioning

confidence: 99%

“…The oil removal rate, %; C 0 : blank hydrocarbon concentration, mg/L; CS: concentration of petroleum hydrocarbon in the culture fluid, mg/L[10].…”

mentioning

confidence: 99%

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Degradation Characteristics of Oil Degrading Candida tropicalis

Zhan¹,

Li²,

Wu³

et al. 2017

OALib

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Objective: The aim of study is to research the degradation characteristics of oil degrading Candida tropicalis (G-94). Methods: Effects of temperature, pH, oil concentration, salt concentration, inoculation amount, N and P source on the oil removal rate of G-94 were studied by single factor test. Meanwhile, the effects of temperature, pH, oil concentration and salt concentration on the oil removal rate of G-94 were optimized by orthogonal design. Result: The orthogonal design showed that the oil removal rate of G-94 could reach 25.83%, 28.56% and 30.90% at 10 d, 20 d and 40 d under the optimal conditions which was 25˚C, initial pH 8.0, oil concentration 1.0%, salt concentration 0.4%, inoculum amount 4%, the optimal N and P were (NH 4 ) 2 SO 4 and K 2 HPO 4 . Conclusion: This experiment studied the degradation characteristics of G-94, which paved the way for the remediation of petroleum contaminated soil.

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Enantioselective Catabolism of Napropamide Chiral Enantiomers in Sphingobium sp. A1 and B2

Huang

Chen

Cheng

et al. 2019

J. Agric. Food Chem.

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Napropamide [N,N-diethyl-2-(1-naphthalenyloxy)propenamide, NAP] is a highly efficient and broad-spectrum amide herbicide. Little is known about the bacterial catabolism of its different enantiomers. Here, we report the isolation of two NAP-degrading strains of Sphingobium sp., A1 and B2, and the different catabolic pathways of different enantiomers in these two strains. Strain A1 dioxygenated NAP at different positions of the naphthalene ring of different enantiomers, leading to the complete degradation of R-NAP while producing a dead-end product from S-NAP. Strain B2 cleaved the amido bonds of both enantiomers, but only the product from S-NAP could be further transformed to form α-naphthol and mineralize in strain B2. The degradation rates of R-NAP and S-NAP in the combination degradation by strains A1 and B2 were 24.8 and 7.5 times that in the single-strain degradation by strain B2 or A1, respectively, showing enhanced synergistic catabolism between strains A1 and B2. This study provides new insights into the enantioselective catabolic network of the chiral herbicide NAP in microorganisms.

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Isolation, Identification and Degradation Characteristics of Oil Degrading Bacterial Strain

Cited by 4 publications

References 15 publications

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Degradation Characteristics of Oil Degrading Candida tropicalis

Enantioselective Catabolism of Napropamide Chiral Enantiomers in Sphingobium sp. A1 and B2

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