Isolation, identification, and determination of antibiogram characteristics of Staphylococcus aureus in cow milk and milk products (yoghurt and cheese) in West Showa Zone, Ethiopia

Feyissa, Negassa; Alemu, Tesfaye; Jirata, Dagim; Dessalegn, Asnake

doi:10.1016/j.idairyj.2022.105503

Cited by 13 publications

(14 citation statements)

References 46 publications

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“…Bacteria, fungi, and viruses are implicated in causing mastitis in bovines. Among the bacterial pathogens, S. aureus has been detemined to cause mastitis in animals, and food poisoning in dairy products (particularly in yogurt and cheese) [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…The other virulence factors include swift development of antibiotic resistance, enterotoxigenicity, and production enzymes such as staphylokinase, lipase, and hemolysins. The antibiotic resistance development nature of the pathogen might be linked to acquiring mobile genetic elements through gene transfer [ 6 , 8 ]. For example, the methicillin-resistant S. aureus (MRSA) strains of the bacterium have been evolved by acquiring genetic components known as staphylococcal cassette chromosome mec (SCCmec), [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Since PBP-2a has less affinity for methicillin, mecA positive S. aureus has developed resistance to methicillin which is drug of choice to treat beta-lactam antibiotic resistant S. aureus infections. In addition the genetic cassette may also contain genes encoding resistance to other groups of antibiotics, and various other virulence factors, including enterotoxins [ 6 , 8 ]. Nevertheless, penicillin resistance of S. aureus might be attributed to penicillinase enzyme production.…”

Section: Introductionmentioning

confidence: 99%

“…Penicillinase enzyme, which is coded by blaZ gene,is responsibe for the hydrolysis of penicillin and penicillin derivatives by breaking the -lactam ring of penicillin [ 10 – 12 ]. Such characteristics of the pathogen might lead to fatal infections caused by the strains because of the lack of alternative antibiotics [ 6 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, nuc gene-positive S. aureus can survive in heat-processed foodstuffs, and/or the toxins they produce might be thermostable. However, although the nuc gene detection in S. aureus has been conducted for species identification [ 14 , 15 ], the nuc gene is not harbored by all strains of S. aureus [ 6 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Determination of Antibacterial Sensitivity Characteristics of Staphylococcus aureus from Lactating Cows in West Shewa Zone, Ethiopia

Feyissa

Alemu

Birri

et al. 2023

Veterinary Medicine International

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Staphylococcus (S.) aureus is one of the etiologies of bovine mastitis, hindering milk production and productivity in dairy farms. This study was aimed at assessing the distribution of bovine mastitis and the isolation rate of S. aureus in milked cows of West Shewa Zone. The clinical mastitis was diagnosed by physical methods including observation and palpation, whereas the subclinical mastitis was tested by the California mastitis test (CMT). All of the cows tested for mastitis were aseptically sampled (teat-milk) for bacteriology. The bacterium was primarily identified based on colony characterization, catalase, coagulase tests, and Gram stain reaction. Finally, MALDI-TOF Biotyper confirmed the species. The antibacterial sensitivity characteristics of the isolates to different antibacterial drugs were tested by the disk diffusion method. The drugs were selected based on the frequent usage in veterinary medicine in the study area. By using particular primers, the presence of the resistance (mecA and blaZ), and thermonuclease (nuc) genes were determined by polymerase chain reaction (PCR). The data were analyzed by R statistical software. The associations between the dependent variables (prevalence of mastitis and S. aureus) and the explanatory variables were analysed by chi-square (χ2) and logistic regression tests at a 95% confidence interval (CI). Accordingly, 258 lactating cows were examined, of which 97 (37.6%) were mastitis positive. Of these mastitis positive cows, 59 (60.8%) were subclinical and 38 (39.2%) were clinical. Among the 258 milk samples, 43 (16.7%) were positive for S. aureus. According to the results of the current investigation, subclinical mastitis was significantly more prevalent than clinical mastitis ( p < 0.05 ). The disease was found varied with the lactation stage of the animal, milking with washed hand, udder washing before milking, and tick infestation of the teat. In comparison to animals from farms with lower number of lactating cows, the prevalence of the bacteria was significantly higher in animals managed in farms with large (OR = 12.58, 95% CI = 2.33–68.54, and p < 0.05 ) and medium (OR = 12.58, 95% CI = 2.33–68.54, and p < 0.05 ) population of lactating cows per herd. The isoation rate of the bacterium was also found significantly higher in tick-infested cows (OR = 27.69, 95% CI = 9.71–93.01, and p < 0.05 ) than tick free cows. The antibiogram tests revealed that the isolates resisted penicillin G and tetracycline group drugs (oxytetracycline and tetracycline). Moreover, the nuc gene was confirmed to be present in all of the examined isolates. However, they were not found harboring blaZ and mecA genes. We concluded that S. aureus is sustaining as a main causative agent of bovine mastitis, and they were resistant to the frequently used antibiotics in public and veterinary medicines in the study areas. Therefore, research-based interventions need to be taken in action to combat the pathogen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and Determination of Antibacterial Sensitivity Characteristics of Staphylococcus aureus from Lactating Cows in West Shewa Zone, Ethiopia

Feyissa

Alemu

Birri

et al. 2023

Veterinary Medicine International

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Antibacterial and Antibiofilm Activity of Zinc Oxide Quantum Dots against Methicillin‐resistant Staphylococcus aureus

Abbas,

Abdullah,

Hallaji

et al. 2024

Part & Part Syst Charact

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Antimicrobial resistance occurs mostly through the ineffective and unauthorized use of antibiotics in both the environment and health care. Biofilms are a new target in the search for new antibacterial agents. Here, the sol‐gel method is used to produce zinc oxide quantum dots (ZnO‐QDs). The ZnO‐QDs show yellow emission at 526 nm under 360 nm excitation. After that, the ability of ZnO‐QDs as an antibacterial and antibiofilm agent against methicillin‐resistant Staphylococcus aureus (MRSA) is examined. The 100 samples are collected from patients with hospital‐acquired infections between December 2020 and October 2021 from Imamen Kadhmiyan Teaching Hospital in Baghdad (Iraq). Out of these 100 samples, 63 are S. aureus and 20 out of 63 are MRSA. The minimum inhibitory concentration assay demonstrates the antibacterial activity of ZnO‐QDs on MRSA strains. Also, results show that 80, 5, and 15% of isolated MRSA strains produce strong, moderate, and weak or very weak biofilm, respectively. The effect of ZnO‐QDs on strong and moderate biofilms reveal that QDs could also inhibit and destroy biofilm produced by MRSA. In addition, the antibiofilm effect of ZnO‐QDs is greater than their antibacterial effect. Generally, this work offers new insights into the development of antibacterial and antibiofilm nanomaterials.

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Screening and application of functional autochthonous starter culture from cured meat, which can reduce nitrite content

Zhang,

Shen,

Meng

et al. 2024

Int Microbiol

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Isolation, identification, and determination of antibiogram characteristics of Staphylococcus aureus in cow milk and milk products (yoghurt and cheese) in West Showa Zone, Ethiopia

Cited by 13 publications

References 46 publications

Isolation and Determination of Antibacterial Sensitivity Characteristics of Staphylococcus aureus from Lactating Cows in West Shewa Zone, Ethiopia

Isolation and Determination of Antibacterial Sensitivity Characteristics of Staphylococcus aureus from Lactating Cows in West Shewa Zone, Ethiopia

Antibacterial and Antibiofilm Activity of Zinc Oxide Quantum Dots against Methicillin‐resistant Staphylococcus aureus

Screening and application of functional autochthonous starter culture from cured meat, which can reduce nitrite content

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