Isolation, identification, and pathogenicity of Lelliottia amnigena causing soft rot of potato tuber in China

Osei, Richard; Yang, Chengde; Cui, Lingxiao; Ma, Ting; Li, Zhezhe; Boamah, Solomon

doi:10.1016/j.micpath.2022.105441

Cited by 14 publications

(12 citation statements)

References 16 publications

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“…A spreading maceration of the tuber tissue, frequently with a creamy consistency that darkens when exposed to air, is a sign of soft rot [ 11 ]. The disease is known as either potato blackleg disease or aerial stem rot when it affects the plant’s aerial components; its symptoms often start as a dark discoloration at the base of stem, at soil line and progress to the stems from infected tubers [ 12 ]. E. carotovora gain entry into the plant through some injuries and degrade its cell wall, followed by tissue maceration through pectolytic enzymes which lead to the soft rotting of the stems and fruits [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

et al. 2023

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In recent years, the biological synthesis of silver nanoparticles has captured researchers’ attention due to their unique chemical, physical and biological properties. In this study, we report an efficient, nonhazardous, and eco-friendly method for the production of antibacterial silver/silver chloride nanoparticles utilizing the leaf extract of Stachys emodi. The synthesis of se-Ag/AgClNPs was confirmed using UV-visible spectroscopy, DPPH free radical scavenging activity, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). An intense peak absorbance was observed at 437 nm from the UV-visible analysis. The Stachys emodi extract showed the highest DPPH scavenging activity (89.4%). FTIR analysis detected various bands that indicated the presence of important functional groups. The SEM morphological study revealed spherical-shaped nanoparticles having a size ranging from 20 to 70 nm. The XRD pattern showed the formation of a spherical crystal of NPs. The antibacterial activity performed against Erwinia carotovora showed the maximum inhibition by centrifuged silver nanoparticles alone (se-Ag/AgClNPs) and in combination with leaf extract (se-Ag/AgClNPs + LE) and leaf extract (LE) of 98%, 93%, and 62% respectively. These findings suggested that biosynthesized NPs can be used to control plant pathogens effectively.

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Section: Introductionmentioning

confidence: 99%

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

et al. 2023

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“…E. mori was isolated from peach fruits (Ahmad et al, 2021), E. persicina was isolated from lettuce, Phaseolus vulgaris and Pisum sativum (Canik Orel, 2020; González et al, 2005, 2007). L. amnigena was isolated from potato (Osei et al, 2022) and S. liquefaciens was isolated from apple (Khalifa, 2022). While preliminary assays confirmed the pectinolytic activity of these non‐ Pectobacterium strains, further in‐depth phylogenetic investigations and host range assays are underway to shed light on the exact taxonomic position and biological features of these strains.…”

Section: Discussionmentioning

confidence: 99%

Field surveys indicate taxonomically diverse Pectobacterium species inducing soft rot of vegetables and annual crops in Iran

Sattar

Osdaghi

2023

Plant Pathology

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Soft rot‐inducing bacterial pathogens are considered among the main biotic constraints on vegetables and annual crops. Most of the bacterial soft rot pathogens are members of Pectobacterium spp. and Dickeya, with the former genus encompassing a continuously increasing number of species varying in geographic distribution and host of isolation. In Iran, Pectobacterium species have rarely been investigated using high‐throughput nucleotide sequence‐based techniques, leaving the genetic diversity of these pathogens mostly undetermined. During 2021, vegetables and annual crops with symptoms of soft rot and maceration were collected from Fars, Hamedan, Isfahan, Khuzestan and Kohgiluyeh‐Boyer‐Ahmad provinces of Iran. Forty‐four pectinolytic bacterial strains were isolated from symptomatic tissues of bell pepper, cabbage, carrot, lettuce, potato and zucchini. The bacterial strains were subjected to pathogenicity and host range assays, specific PCR tests and phylogenetic analysis of three housekeeping genes (dnaX, gapA and leuS). All strains were pathogenic on their host of isolation. Among the 44 pectinolytic strains, 26 strains were identified as members of Pectobacterium based on PCR using the primer pair Y1/Y2. Multilocus sequence analysis confirmed the latter bacterial strains belonging to the genus Pectobacterium and revealed that the strains scattered into four species: P. brasiliense (five strains), P. carotovorum (eight strains), P. polaris (five strains) and P. versatile (eight strains). P. polaris and P. versatile have not previously been reported in the country. Host range assays showed that P. brasiliense and P. carotovorum have a broader host range than the other two species.

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“…The symptoms were consistent with those reported for plants infected with certain genera of the E . cloacae complex (Abd‐Elhafeez et al, 2018; Osei et al, 2022), namely watery lesions that appeared on its roots. To the best of our knowledge, this is the first report of L .…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of pathogenicity of Lelliottia nimipressuralis causing soft rot of Codonopsis pilosula (dangshen) roots in China

et al. 2022

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The roots of Codonopsis pilosula, a crucial ingredient in Chinese medicine, are prone to soft rot disease soon after transplanting seedlings in the second year. This almost always causes severe losses in yield at harvest, with severe economic consequences for farmers of this perennial vine. However, the pathogens involved remain understudied, although some gram‐negative bacteria of the Enterobacteriaceae family are well‐known pathogens of soft rot in many other plants. Here, the isolate B5‐2‐1‐30 was suspected of being a key pathogen of C. pilosula in China according to counts of bacteria from diseased, healthy and adjacent tissues of roots. Results of morphological and phylogenetic analyses, including microscopic observations, in addition to a multilocus sequence analysis (MLSA) of the concatenated partial 16S rDNA, rpoB, atpD and gyrB genes, revealed that this isolate belongs to Lelliottia nimipressuralis. In vitro and in vivo pathogenicity tests showed that L. nimipressuralis B5‐2‐1‐30 could cause root soft rot in seedlings of C. pilosula, with symptoms similar to those observed in the field. Further experiments suggested the typical symptoms of softened rotting roots and wilting shoots arose from phloem necrosis and vessel plugging, as inferred by observed anatomical characteristics of roots, providing clues for elucidating the infection mechanism of phytopathogens in L. nimipressuralis.

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Isolation, identification, and pathogenicity of Lelliottia amnigena causing soft rot of potato tuber in China

Cited by 14 publications

References 16 publications

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

Field surveys indicate taxonomically diverse Pectobacterium species inducing soft rot of vegetables and annual crops in Iran

Identification and characterization of pathogenicity of Lelliottia nimipressuralis causing soft rot of Codonopsis pilosula (dangshen) roots in China

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