Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste

Al-Marri, Salih; Eldos, Haneen I.; Ashfaq, Mohammad Y.; Saeed, Shafqat; Skariah, Sini; Varghese, Lity Alen; Mohamoud, Yasmin Ali; Sultan, Ali A.; Raja, M. Nobin

doi:10.1016/j.btre.2023.e00804

Cited by 13 publications

(4 citation statements)

References 82 publications

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“…This agreed with the research of Haque et al (2020), who noted that all P. aeruginosa strains contained OPrI and OPrL genes. Similarly, Al-Marris et al (2023), noted that P. aeruginosa strains contained same genes and exhibit remarkable potential to produce biosurfactants using various carbon sources. For 463 (F/R) pair of primers, there was no amplification.…”

Section: Resultsmentioning

confidence: 96%

“…Biological means are employed to remediate oil polluted, hence production of biosurfactants. Several studies have shown that different microorganisms such as Pseudomonas, Bacillus, Streptomyces, Azotobacter, Citrobacter, Stenotrophomonas, Lactobacillus, etc present in oil contaminated sites have potentials to degrade different crude oil products, utilize them as energy sources, and sometimes encase them in their body biomass leading to production of active surfactants (Araújo et al, 2019;Farias et al, 2021;Talreja et al, 2022;Al-Marris et al, 2023). Biosurfactants are surface-active metabolites produced by microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of biosurfactant-producing bacteria obtained from oil-polluted soil samples in Abakaliki, Ebonyi State, Nigeria

Nwachi,

Okoye,

Nwakaeze

et al. 2024

Arch. Agric. Environ. Sci.

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Several human activities have led to environmental pollution with crude oil and other related hydrocarbons. There are various technologies of remediating oil-polluted environments, but the use of biological agents such as biosurfactants are widely practiced, and are generally considered as safe and eco-friendly. This study aimed at isolating and identifying biosurfactant-producing bacteria obtained from oil polluted soil samples in Abakaliki, Ebonyi State, Nigeria using molecular techniques. Six waste oil-contaminated soil samples were collected from mechanic and generator repair workshops, and were analyzed using standard microbiological methods. Nine bacterial isolates were obtained from the waste oil-contaminated soil samples, and were suspected to be Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. Molecular identification of the biosurfactant-producing isolates was also carried out using PCR and the genes responsible for biosurfactant production targeted. Oil spreading technique was used to detect the oil displacement activity (ODA) of the isolates. The emulsification index (E24) was calculated and the ability of the extracted biosurfactant to emulsify the hydrocarbons was determined. Pseudomonas aeruginosa had the highest ODA of 28.1 cm while Staphylococcus aureus had the least ODA of 5 cm. Pseudomonas aeruginosa showed E24 of 70.1%, Bacillus subtilis 57.1% while S. aureus showed 32.5%. Hence, Pseudomonas and Bacillus species have good potentials for biosurfactant production, and can be harnessed and utilized in the cleanup of oil polluted soil.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Molecular identification of biosurfactant-producing bacteria obtained from oil-polluted soil samples in Abakaliki, Ebonyi State, Nigeria

Nwachi,

Okoye,

Nwakaeze

et al. 2024

Arch. Agric. Environ. Sci.

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“…Prior studies have indicated that these metals can serve as electron acceptors in soils with limited oxygen content, potentially enhancing bioremediation activity. While oxygen typically functions as the primary electron acceptor under aerobic conditions, microorganisms can utilize Co and Mn as alternative electron acceptors in anaerobic environments [ 31 ]. Microorganisms can produce biosurfactants that reduce surface and interfacial tension.…”

Section: Resultsmentioning

confidence: 99%

Construction of Shale Gas Oil-Based Drilling Cuttings Degrading Bacterial Consortium and Their Degradation Characteristics

Fan,

Gong,

et al. 2024

Microorganisms

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Oil-based drilling cuttings (OBDCs) contain petroleum hydrocarbons with complex compositions and high concentrations, which have highly carcinogenic, teratogenic, and mutagenic properties. In this study, three highly efficient petroleum hydrocarbon-degrading bacteria were screened from OBDCs of different shale gas wells in Chongqing, China, and identified as Rhodococcus sp. and Dietzia sp. Because of their ability to degrade hydrocarbons of various chain lengths, a new method was proposed for degrading petroleum hydrocarbons in shale gas OBDCs by combining different bacterial species. Results showed that the bacterial consortium, consisting of the three strains, exhibited the highest degradation rate for petroleum hydrocarbons, capable of degrading 74.38% of long-chain alkanes and 93.57% of short-chain alkanes, respectively. Moreover, the petroleum hydrocarbon degradation performance of the bacterial consortium in actual OBDCs could reach 90.60% in the optimal conditions, and the degradation kinetic process followed a first-order kinetic model. This study provides a certain technical reserve for the bioremediation of shale gas OBDCs.

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“…In all over the world, some studies on biosurfactant‐producing microorganisms have achieved certain results 5–8 . These microbes have been shown that can utilize glucose, olive, soy bean, diesel oil, etc.…”

Section: Introductionmentioning

confidence: 99%

Biosurfactant production by a crude oil‐utilizing bacterium towards petroleum hydrocarbon biodegradation applications

Kieu,

Nguyen,

Nguyen

et al. 2024

Vietnam Journal of Chemistry

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Biosurfactants produced by microorganisms are green amphiphilic biomolecules. They have numerous advantages compared to chemical surfactants including a lower toxicity, better environmental compatibility and effective and stable properties under extreme conditions such as a wide range of temperature, pH and salinity. A highly bio‐surfactant‐producing strain of Bacillus, VTVK15 was selected among the Bacillus isolates from marine environment and oil contaminated sites in Ba Ria—Vung Tau. Sequence analysis of 16S rDNA showed that the VTVK15 strain was 99% similar with 16s rDNA sequence of Bacillus megaterium. The suitable conditions for the bio‐surfactant production by the strain VTVK15 were 37 °C, pH 7; 2% w/v and 0.4% w/v for temperature, initial solution pH, concentration of carbon substrate (crude oil), and concentration of nitrogen substrate ((NH4)2SO4), respectively. The emulsification index (E24) increased from 51.8% to 67.7% with suitable conditions. The petroleum hydrocarbon degradation (TPH) efficiency by the strain Bacillus megaterium VTVK15 in crude oil estimated using TPH analysis was 72% after 14 days. These results revealed that the strain VTVK15 exhibited a tremendous potential for bioremediation of petroleum hydrocarbon contaminants.

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Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste

Cited by 13 publications

References 82 publications

Molecular identification of biosurfactant-producing bacteria obtained from oil-polluted soil samples in Abakaliki, Ebonyi State, Nigeria

Molecular identification of biosurfactant-producing bacteria obtained from oil-polluted soil samples in Abakaliki, Ebonyi State, Nigeria

Construction of Shale Gas Oil-Based Drilling Cuttings Degrading Bacterial Consortium and Their Degradation Characteristics

Biosurfactant production by a crude oil‐utilizing bacterium towards petroleum hydrocarbon biodegradation applications

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