Isolation, Identification of Endophytic Fungi from Mangrove Roots along the Coast of South Andaman Sea,Andaman and Nicobar Islands,India

Thorati, Madhusudan; Mishra, Jayant Kumar; Sandeep, K.

doi:10.4172/2324-8661.1000157

Cited by 12 publications

(9 citation statements)

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“…Besides this genus, Aspergillus and Penicillium were also found predominant. Aspergillus was the dominant endophyte isolated from the roots of Rhizophora apiculata, Rhizophora mucronata and Bruguiera gymnorrhiza Andaman and Nicobar Islands (Thorati;. Penicillium spp.…”

Section: Discussionmentioning

confidence: 99%

Diversity and bioactive profile of endophytic mycoflora in mangroves

Revathy

Mohan

Kesavan

et al. 2022

Preprint

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The present study deals with the mangrove associated endophytic fungi and their bioactivity profile. The fungal endophytes were isolated from the leaves of three mangrove species (Aegiceras corniculatum, Lumnitzera racemosa and Ceriops tagal) and identified by morphological and molecular methods (Internal Transcribed Spacer and β-tubulin (Ben A) sequencing). The isolates mainly belonged to Ascomycota (95%) and the Basidiomycota comprised only 5%. They come under 5 classes, 11 orders, 16 genera and 25 species. Under Ascomycota, four classes, viz., Sordariomycetes (44.5%), Eurotiomycetes (32.46%), Dothideomycetes (17.28%) and Saccharomycetes (1.83%) comprising 10 orders were recorded. Under Basidiomycota a single class viz., Agaricomycetes (3.93%) comprising 1 order (Agaricales) was observed. Colletotrichum (30.2%) was the dominant genus followed by Aspergillus (17.8%), Penicillium (14.1%), Phyllosticta (9.1%) etc. Penicillium citrinum was the dominant fungus (22%) in A. corniculatum; Colletotrichum siamense (31%) in L. racemosa and Aspergillus sydowii (35%) in C. tagal (Fig. 3). Among the isolates, the Colonization frequency (CF%) was maximum for Colletotrichum siamense (18%) followed by Phyllosticta capitalensis (11.6%). Penicillium citrinum was found in all the three plant species. Isolates from Ceriops tagal were found to exhibit higher antibacterial as well as hydrolytic enzyme production potential. Aspergillus montevidensis EF 30, Cladosporium oxysporum EF 316, Colletotrichum siamense EF 272, Penicillium chrysogenum EF 362, Colletotrichum fruticola EF 22, Ascotricha chartarum EF 374, Aspergillus sydowii EF 239, Talaromyces purpurogenus EF 313, Meyerozyma caribbica EF 347 and Schizophyllum commune EF 309 were segregated as potential strains for hydrolytic enzyme production. A. aculeatus EF 10, T. purpurogenus EF 313, C. oxysporum EF 316, C. fruticola EF 74, P. capitalensis EF 187, A. sydowi EF 226 and P. chrysogenum EF 363 showed notable antimicrobial property. T purprogenus EF 313 endowed with pigment (red) production has potential for application in dyeing industry. During the current study, 25 different species of endophytic fungal species could be recovered from three host mangrove plants and some of them were having high bioactive potential for possible commercial applications with respect to bioremediation and as antimicrobials in medicine and aquaculture.

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Section: Discussionmentioning

confidence: 99%

Diversity and bioactive profile of endophytic mycoflora in mangroves

Revathy

Mohan

Kesavan

et al. 2022

Preprint

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“…Molecular identification confirms that isolate DM2 is Aspergillus minisclerotigenes. Fungi from Aspergillus genera previously reported as endophytic fungi and able to produce antimicrobial compounds [21,22]. Rani et al [21] reported that ethyl acetate extract of Aspergillus nomius and Aspergillus oryzae isolated from Calotropis procera have antimicrobial activity against Salmonella typhi, Shigella lexneri, and Serratia marcescens.…”

Section: Morphological and Molecular Identities Of Endophytic Fungimentioning

confidence: 99%

“…Rani et al [21] reported that ethyl acetate extract of Aspergillus nomius and Aspergillus oryzae isolated from Calotropis procera have antimicrobial activity against Salmonella typhi, Shigella lexneri, and Serratia marcescens. Thorati and Mishra [22] reported that the crude extract of Aspergillus niger isolated from the stilt roots of Rhizophora apiculata able to inhibit five human bacterial pathogens.…”

Section: Morphological and Molecular Identities Of Endophytic Fungimentioning

confidence: 99%

Antibacterial Activity of Endophytic Fungi Isolated from Pometia pinnata J. R. Forst & G. Forst Against Escherichia coli

Prasetyani¹,

Setyaningsih²,

Susilowati³

2021

Advances in Biological Sciences Research

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The emerging of antimicrobial resistance become a global concern, encouraging researchers to find out alternative antimicrobial compounds. Endophytic fungi associated with medicinal plants become promising sources because of their ability to produce medical bioactive. This study aimed at bioprospecting fungal endophytes from an ethnobotanical medicinal plant, Pometia pinnata J. R. Forst & G Forst for their antimicrobial activity. Endophytic fungi isolated from leaf, stem, and root aseptically. Ten selected endophytes were cultured and then extracted using ethyl acetate. Screening antibacterial activity of ethyl acetate extract carried out by paper disk diffusion assay against Escherichia coli ATCC 25922. TLC-bioautography assay was used to study the type of secondary metabolite which has antibacterial activity. Identification of endophytic fungi based on morphology and ITS sequence using ITS1 and ITS4 primers. The result showed that two isolates have antibacterial activity but only one isolate exhibits strong antibacterial activity with a diameter of inhibition zone 21 mm. The isolate was identified as Aspergillus minisclerotigenes with 100% identity of the ITS sequence using BLAST software on NCBI. TLC assay indicated that ethyl acetate extract of A. minisclerotigenes contains alkaloids, phenols, flavonoids, and terpenoids while TLC bioautography showed that its phenolic compound appeared antibacterial activity.

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“…Ethics approval and consent to participate Not applicable. Koehn and Garrison 1981, Kuthubutheen 1984, Rai et al 1969, Nayak and Anandhu 2017 Aspergillus fumigatus Living leaves of Avicennia germinans India, Japan, Hawaii, Malaysia, Pakistan Chowdhery and Rai 1980, Ito and Nakagiri 1997, Kuthubutheen 1984, Lee and Baker 1972, Rai et al 1969, Tariq et al 2006, Thorati et al 2016 Aspergillus Kumaresan and Suryanarayan 2002, Suryanarayanan and Kumaresan 2000, Suryanaryanan et al 1998, Tariq et al 2006, Nayak and Anandhu 2017, Chowdhery and Rai 1980, Ito and Nakagiri 1997, Koehn and Garrison 1981, Kuthubutheen 1984, Newell, Steven Y. 1976.…”

mentioning

confidence: 99%

“…Aspergillus fumigatus Living leaves of Avicennia germinans India, Japan, Hawaii, Malaysia, Pakistan Chowdhery and Rai 1980, Ito and Nakagiri 1997, Kuthubutheen 1984, Lee and Baker 1972, Rai et al 1969, Tariq et al 2006, Thorati et al 2016 Aspergillus Kuthubutheen 1981, Kuthubutheen 1984, Meyers et al 1965, Newell 1976, Newell 1973, Newell and Fell 1980 Bremer 1995, Poonyth et al 2000, Kuthubutheen 1984, Lee et al Lee1973, Newell 1976, Rai et al 1969, Tariq et al 2006 Tryblidaria pongamiae…”

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confidence: 99%

Three saprobic Dothideomycetes from the aerial parts of mangrove trees with polyphenism in Striatiguttula

Kumar

Thambugala

Sarma

et al. 2021

Preprint

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Fungi inhabiting the aerial parts of two mangrove trees, Nypa fruticans, and Rhizophora apiculata, were studied from the central region of Thailand, utilizing morpho-molecular characteristics. Three different fungal taxa were isolated including Rhytidhysteron kirshnacephalus sp. nov., Lasiodiplodia citricola and Striatiguttula phoenicis. Sexual morphs are reported for these three taxa and the asexual morph of Striatiguttula phoenicis is identified based on molecular data. This is the first asexual morph report for the genus Striatiguttula as well as the family Striatiguttulaceae. The new isolate of Striatiguttula phoenicis differs slightly from other extant species in the genus in terms of measurements of ascomata, asci, ascospores, and thickness of peridium. Also, a pigmented hamathecium was observed in this species. The morphological results are congruent to the phylogenetic results of previous studies and support Striatiguttula phoenicis as a new host record from Nypa fruticans. Rhytidhysteron kirshnacephalus was collected from dead twigs of a standing Rhizophora apiculata in Cha-am and it has significant morphological and molecular differences to support its establishment as a novel taxon. Phylogenetically, Rhytidhysteron kirshnacephalus forms a sister clade to Rh. magnoliae, but has different ascomatal characters, including, smooth margins without striations and black pruina. Lasiodiplodia citricola is another species from Cha-am and a new record from Thai mangroves. Detailed descriptions of the isolates, along with their potential ecological roles, are provided. We have also provided the occurrence of fungi from the aerial parts of mangrove trees worldwide.

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Isolation, Identification of Endophytic Fungi from Mangrove Roots along the Coast of South Andaman Sea,Andaman and Nicobar Islands,India

Cited by 12 publications

References 0 publications

Diversity and bioactive profile of endophytic mycoflora in mangroves

Diversity and bioactive profile of endophytic mycoflora in mangroves

Antibacterial Activity of Endophytic Fungi Isolated from Pometia pinnata J. R. Forst & G. Forst Against Escherichia coli

Three saprobic Dothideomycetes from the aerial parts of mangrove trees with polyphenism in Striatiguttula

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