Isolation, Identification Similarity and Qualitative Expression of Metallothionein Gene in IR-Bagendit Rice (Oryza sativa)

Abstract

Metallothionein (MTs) is an enzyme that plays a role in the binding of metals in plants. Various types of rice have been known to contain MTs and IR-Bagendit rice leaves have the highest MTs protein content compared to other rice varieties. However, MTs coding gene in IR-Bagendit rice variety is still unknown. OsRAC1 gene is reported as the down-regulator of MTs and there is an analogous gene for MTs-like gene using RAP1 and RAP2 primers in various plants. This study aimed to isolate, identification of similarity, and analysis of qualitative expression of MTs gene in IR-Bagendit rice as compared to Inpari, IR-36, and IR-34. The steps of this research were DNA isolation, PCR in OsRAC1 gene, RNA isolation and cDNA reverse transcription using primer RP1 and RP2, and agarose gel electrophoresis. Amplification quality of OsRAC1 gene in IR-Bagendit, Inpari, IR-36, and IR-34 showed the same result. Qualitative expression of MTs by reverse transcription showed that IR-Bagendit has the highest MTs-like gene compared to other samples.

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

“…Such conditions could damage kidney cells. 15,5 The decrease in the body's antioxidants and the increase in free radicals will cause oxidation and escalate lipid peroxidation. Unstable lipid peroxidation can damage three types of compounds that significantly maintain cell integrity; they are cell membranes, proteins, and DNA.…”

“…Therefore, metallothionein can be used as a biomarker of heavy metal exposure. 15 This study aims to determine the functions of IR-Bagendit rice leaf infusion from Blora to prevent degeneration and necrosis of renal tubular cells.…”

Blood Profile of Rattus Nurvegicus Exposed to HgCl2 and Received Combination of IR Bagendit Rice Leaves-Infused Water and Young Coconut Water

“…IR Bagendit rice leaves-infused water is rich in the metallothionein protein (Santosa et al, 2018;Santosa et al, 2020) and has many sulfhydryl groups that function in the HG detoxication mechanism. A large number of sulfhydryl groups in the metallothionein protein affects the protein's ability to bind Hg.…”

“…The binding coordination of each metal ion of the Cys forms a tetrahedral structure of tetrathionate (Nezhad RM, Shahpiri A, 2013). A previous study has proven that metallothionein genes in chromosome 3 function as a protein induced by environmental stresses, such as the presence of metallic contaminants (Santosa et al, 2020) Coconut water drink is found in tropical countries, such as Indonesia. Moreover, coconut water has been commonly consumed as a thirst-quenching drink.…”