Isolation of 19 strains of Malassezia dermatis from healthy human skin in Korea

Abstract

This research was conducted on the cultured samples of 160 healthy men and women aged 0-80 years without any skin disease. Nineteen clinical isolates of Malassezia dermatis showed positive in a catalase test and all grew in 0.5% Tween-60 and 0.1% Tween-80 added to 2% glucose/1% peptone culture medium. Round and ellipsoidal yeast cells and budding of the yeast cells were observed by microscopy, resembling Malassezia sympodialis, Malassezia furfur and Malassezia nana. The 26S rDNA polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) pattern was the same as for M. dermatis (JCM 11348), the standard strain. 26S rDNA and ITS1 sequencing were performed for exact identification, showing 99% accordance with M. dermatis (AB070361), M. dermatis (AB070356), confirming the species to be new and first to be reported in Korea. Taking a molecular biological classification approach by analyzing the 26S rDNA PCR-RFLP patterns, we have successfully isolated 19 cases of M. dermatis- the first in Korea.

Distribution ofMalasseziaSpecies on the Scalp in Korean Seborrheic Dermatitis Patients

“…There are five reports of the successful use of 26S rDNA PCR-RFLP for the identification of Malassezia species [13][14][15][16][17] .…”

Pityrosporum folliculitis in an immunocompetent patient: clinical case description

“…Although yeasts of the genus Malassezia spp. form part of the normal flora of the skin, in some situations the organisms may cause or aggravate diseases, including pityriasis versicolor, pityrosporum folliculitis, and seborrheic dermatitis [6,7]. In immunocompromised patients (such as in AIDS, Hodgkin's disease, chronic kidney failure, transplant patients using immunosuppressants, chronic use of steroids), Malassezia spp.…”

Progress inMalasseziaResearch in Korea

“…M. globosa , M. sympodialis , and M. restricta were the most common causative agents in patients with PV, Malassezia folliculitis, and seborrheic dermatitis, respectively. Lee et al 56 isolated 19 strains of M. dermatis from healthy human skin in Korea by using 26S rDNA PCR-RFLP.…”