Isolation of a bchl c mutant from<i>Chlorobium</i>with bchl d by cultivation at low light intensity

Broch-Due, Margrethe; Ormerod, John G.

doi:10.1111/j.1574-6968.1978.tb01953.x

Cited by 39 publications

(2 citation statements)

References 7 publications

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“…Several studies have also investigated adjustments in the light-harvesting apparatus in response to alterations in light intensity (Borrego and Garcia-Gil 1994;Borrego et al 1999;Broch-Due and 1978;Overmann et al 1992;Smith and Bobe 1987). The function of the chlorosome is to collect sufficient light energy to drive charge separation of the BChl a special pair reaction center which is ultimately used to produce reducing equivalents in the form of reduced Fd and NADPH.…”

Section: Discussionmentioning

confidence: 99%

Chlorobaculum tepidum regulates chlorosome structure and function in response to temperature and electron donor availability

et al. 2008

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Green sulfur bacteria (GSB) rely on the chlorosome, a light-harvesting apparatus comprised almost entirely of self-organizing arrays of bacteriochlorophyll (BChl) molecules, to harvest light energy and pass it to the reaction center. In Chlorobaculum tepidum, over 97% of the total BChl is made up of a mixture of four BChl c homologs in the chlorosome that differ in the number and identity of alkyl side chains attached to the chlorin ring. C. tepidum has been reported to vary the distribution of BChl c homologs with growth light intensity, with the highest degree of BChl c alkylation observed under low-light conditions. Here, we provide evidence that this functional response at the level of the chlorosome can be induced not only by light intensity, but also by temperature and a mutation that prevents phototrophic thiosulfate oxidation. Furthermore, we show that in conjunction with these functional adjustments, the fraction of cellular volume occupied by chlorosomes was altered in response to environmental conditions that perturb the balance between energy absorbed by the light-harvesting apparatus and energy utilized by downstream metabolic reactions.

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Section: Discussionmentioning

confidence: 99%

Chlorobaculum tepidum regulates chlorosome structure and function in response to temperature and electron donor availability

et al. 2008

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“…Chlorosomes usually contain only one type of self-assembling BChl (i.e., only BChl c , d , or e but not mixtures). , However, there are a few known exceptions in which two types of these molecules occupy the same chlorosome. − In principle, having many species of pigments within a single chlorosome would expand the spectral range for light harvesting and therefore enhance photosynthetic output. However, because such a situation does not seem to be widespread in nature, it is difficult to ascertain whether such a situation is energetically functional or optimal.…”

Section: Introductionmentioning

confidence: 99%

Polymer–Chlorosome Nanocomposites Consisting of Non-Native Combinations of Self-Assembling Bacteriochlorophylls

Orf¹,

Collins

Niedzwiedzki³

et al. 2017

Langmuir

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Chlorosomes are one of the characteristic light-harvesting antennas from green sulfur bacteria. These complexes represent a unique paradigm: self-assembly of bacteriochlorophyll pigments within a lipid monolayer without the influence of protein. Because of their large size and reduced complexity, they have been targeted as models for the development of bioinspired light-harvesting arrays. We report the production of biohybrid light-harvesting nanocomposites mimicking chlorosomes, composed of amphiphilic diblock copolymer membrane bodies that incorporate thousands of natural self-assembling bacteriochlorophyll molecules derived from green sulfur bacteria. The driving force behind the assembly of these polymer-chlorosome nanocomposites is the transfer of the mixed raw materials from the organic to the aqueous phase. We incorporated up to five different self-assembling pigment types into single nanocomposites that mimic chlorosome morphology. We establish that the copolymer-BChl self-assembly process works smoothly even when non-native combinations of BChl homologues are included. Spectroscopic characterization revealed that the different types of self-assembling pigments participate in ultrafast energy transfer, expanding beyond single chromophore constraints of the natural chlorosome system. This study further demonstrates the utility of flexible short-chain, diblock copolymers for building scalable, tunable light-harvesting arrays for technological use and allows for an in vitro analysis of the flexibility of natural self-assembling chromophores in unique and controlled combinations.

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Chloroflexus

Pierson¹,

Castenholz²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Chlo.ro.flex' us. Gr. adj. chloros green; L. masc. n. flexus a bending; M.L. masc. n. Chloroflexus green bending. Chloroflexi / “Chloroflexi” / “Chloroflexales” / “Chloroflexaceae” / Chloroflexus Filaments of indefinite length ; cells 0.5–1.5 × 2–6 µm; none differentiated (Figure 1). Cell division by fission, no branching. No internal proliferations of cell membrane, except mesosomes; chlorosomes present when anaerobically grown. Motile by gliding . Thin sheath may be present. Stain Gram‐negative, but cell wall not typical of Gram‐negative bacteria. No flagella. The mol % G + C of the DNA is : 53.1–54.9 or 56.7–57.1. Type species : Chloroflexus aurantiacus Pierson and Castenholz 1974a, 7.

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Isolation of a bchl c mutant fromChlorobiumwith bchl d by cultivation at low light intensity

Cited by 39 publications

References 7 publications

Chlorobaculum tepidum regulates chlorosome structure and function in response to temperature and electron donor availability

Chlorobaculum tepidum regulates chlorosome structure and function in response to temperature and electron donor availability

Polymer–Chlorosome Nanocomposites Consisting of Non-Native Combinations of Self-Assembling Bacteriochlorophylls

Chloroflexus

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