Isolation of a free radical‐scavenging antioxidant from water spinach (Ipomoea aquatica Forsk)

Abstract

Ipomoea aquatica Forsk, a green leafy vegetable that is a rich source of vitamins and amino acids with many health benefits, has been explored for the isolation and identification of its bioactive compounds. Activity-guided repeated fractionation of a methanol extract on a silica gel column followed by an XAD column yielded a compound that exhibited antioxidant activity with an EC 50 value of 83 ± 1.02 µg ml −1 reaction mixture. It also showed very strong lipid peroxidation-inhibitory activity in a liposome model system with an EC 50 value of 72.2 ± 0.9 µg ml −1 . However, it showed negligible metal-chelating activity. Based on UV, 2D nuclear magnetic resonance and gas chromatography/mass spectrometry studies, the compound was tentatively identified to be 7-O-β-D-glucopyranosyl-dihydroquercetin-3-O-α-D-glucopyranoside. This is the first report on the antioxidant properties of I aquatica leaf extracts.

Anti–bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh

“…It was found that no investigation has been carried out for cytotoxic activities till now among our studied fourteen vegetables except Diaplazium esculentum and Ipomoea aquatica. In the present study, Ipomoea aquatica showed strong cytotoxic activity which is in accordance with the result obtained earlier [32] . On the other hand Diaplazium esculentum also displayed potent cytotoxic potential in our observations but in another report it was found to have no cytotoxic activity [77] .…”

“…Glinus oppositifolius (L.) was found to contain immunomodulating peptic polymer, terpinoid saponine having antiprotozoal activity, and bioactives having antioxidant property [27][28][29] . Ipomoea aquatica Forsk was reported for hypoglycemic activity, cytotoxic activity [30][31][32] . Lagenaria siceraria was reported for its diuretic activity [33] , antihypertensive and cardioprotective effect [34] .…”

Antioxidant activities of fractions from longan pericarps

“…TPC for five fractions from longan pericarp was decreased in the following order LPEF > LPDF > LPBF > LPCE > LPR. Several reports (Duan et al, 2007;Li & Jiang, 2007;Prasad et al, 2005) have conclusively shown a close relationship between total phenolic content and antioxidant activity since phenolic compounds serving as hydrogen-donating agents. Sarikurkcu et al (2008) reported that dietary plants possessing substantial phenolic compounds may be health-benefit due to their antioxidant properties.…”

Effects of pectinase clarification treatment on phenolic compounds of pummelo (Citrus grandis l. Osbeck) fruit juice

“…Phenolic present in fruits and vegetables have received considerable attention because of their potential antioxidant activity. Phenolic compounds undergo a complex redox reaction with the phosphotungstic and phosphor-molybdic acids present in the Folin-Ciocalteu reagent (Prasad et al 2005). It was found that Ledang variety had a considerably lower total phenolic contents compared to Tambun variety (p <0.05).…”