Isolation of a goose parvovirus from swan and its molecular characteristics

Shao, Hongbo; Y, Lv; Ye, Jianping; Qian, Kun; Jin, Wei; A, Qin

doi:10.4149/av_2014_02_194

Cited by 16 publications

(13 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Normally, GPVs have been found in goslings, Muscovy ducklings [5, 8], swans [9], and Anser cygnoides [10], whereas MDPV has only been discovered in Muscovy ducklings. MDPV infection was initially described by Professor Lin in our laboratory in the early 1980s [11].…”

Section: Introductionmentioning

confidence: 99%

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

et al. 2018

View full text Add to dashboard Cite

BackgroundMuscovy duck parvovirus (MDPV) causes high mortality and morbidity in Muscovy ducks, with the pathogenesis of the virus still unknown in many respects. Specific MDPV detection is often rife with false positive results because of high identity at the genomic nucleotide level and antigenic similarity with goose parvovirus (GPV). The objective of this study was to develop a sensitive, highly specific, and repeatable TaqMan-based real-time PCR (qPCR) assay for facilitating the molecular detection of MDPV.ResultsThe specific primers and probe were designed based on the conserved regions within MDPVs, but there was a variation in GPVs of the nonstructural (NS) genes after genetic comparison. After the optimization of qPCR conditions, the detection limit of this qPCR assay was 29.7 copies/μl. The assay was highly specific for the detection of MDPV, and no cross-reactivity was observed with other non-targeted duck-derived pathogens. Intra- and inter-assay variability was less than 2.21%, means a high degree of repeatability. The diagnostic applicability of the qPCR assay was proven that MDPV-positive can be found in cloacal swabs samples, Muscovy duck embryos and newly hatched Muscovy ducklings.ConclusionsOur data provided incidents that MDPV could be possible vertically transmitted from breeder Muscovy ducks to Muscovy ducklings. The developed qPCR assay in the study could be a reliable and specific tool for epidemiological surveillance and pathogenesis studies of MDPV.

show abstract

Section: Introductionmentioning

confidence: 99%

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Previous observation and research indicated that the goose parvovirus can infect both goslings and ducklings. Then, goose parvovirus was isolated from swans in China in 2012 [9] . Earlier studies have confirmed that goose parvovirus genome remained genetically stable in the field.…”

Section: Discussionmentioning

confidence: 99%

Çin’de Anser cygnoides’ten Izole Edilen Kaz Parvovirusu’nun Tam Genomik Dizilimi

Wan¹,

FU²,

FU³

et al. 2015

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

In the present study, we sequenced and analyzed the complete genome of goose parvovirus isolates derived from Anser cygnoides. Comparing with other GPV isolates, the results indicated that the event may cause by GPV direct infection to Anser cygnoides. These findings suggest that the Anser cygnoides could serve as a potential host for GPV and enable us to understand the molecular characteristics and evolutionary diversity of GPV. Keywords: Goose parvovirus, Anser cygnoides, Genome, Analysis Çin'de Anser cygnoides'ten Izole Edilen Kaz Parvovirusu'nun Tam Genomik Dizilimi ÖzetBu çalışmada, Anser cygnoides'ten elde edilen kaz parvovirusu genomunun dizilimi yapıldı ve analiz edildi. Diğer GPV izolatlarıyla kıyaslandığında, sonuçlar olayın doğrudan Anser cygnoides'e bağlı bir GPV enfeksiyonuna bağlı olabileceğini gösterdi. Bu bulgulara göre, Anser cygnoides'in GPV için potansiyel bir konakçı (ev sahibi) olarak hizmet edebildiği ve GPV'nin moleküler özelliklerinin ve evrimsel çeşitliliğinin anlaşılmasını sağlayabildiği kanısına varıldı.

show abstract

“…8,[18][19][20] This consistency in genome length is similar to the genomes of other parvoviruses, which also package single-stranded DNA into an icosahedral capsid of similar size. [21][22][23][24] This suggests that the maximum packaging capacity of members of the Parvoviridae family is approximately 4.5-5.5 kb.…”

Section: Packaging Capacity Of Viruses Of the Parvoviridae Familymentioning

confidence: 99%

Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids

Chamberlain

Riyad

Weber

2016

Human Gene Therapy Methods

118

View full text Add to dashboard Cite

Recombinant adeno-associated virus vectors (rAAV) are being explored as gene delivery vehicles for the treatment of various inherited and acquired disorders. rAAVs are attractive vectors for several reasons: wild-type AAVs are nonpathogenic, and rAAVs can trigger long-term transgene expression even in the absence of genome integration-at least in postmitotic tissues. Moreover, rAAVs have a low immunogenic profile, and the various AAV serotypes and variants display broad but distinct tropisms. One limitation of rAAVs is that their genome-packaging capacity is only *5 kb. For most applications this is not of major concern because the median human protein size is 375 amino acids. Excluding the ITRs, for a protein of typical length, this allows the incorporation of *3.5 kb of DNA for the promoter, polyadenylation sequence, and other regulatory elements into a single AAV vector. Nonetheless, for certain diseases the packaging limit of AAV does not allow the delivery of a full-length therapeutic protein by a single AAV vector. Hence, approaches to overcome this limitation have become an important area of research for AAV gene therapy. Among the most promising approaches to overcome the limitation imposed by the packaging capacity of AAV is the use of dual-vector approaches, whereby a transgene is split across two separate AAV vectors. Coinfection of a cell with these two rAAVs will then-through a variety of mechanismsresult in the transcription of an assembled mRNA that could not be encoded by a single AAV vector because of the DNA packaging limits of AAV. The main purpose of this review is to assess the current literature with respect to dual-AAV-vector design, to highlight the effectiveness of the different methodologies and to briefly discuss future areas of research to improve the efficiency of dual-AAV-vector transduction.

show abstract

Isolation of a goose parvovirus from swan and its molecular characteristics

Cited by 16 publications

References 10 publications

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

Çin’de Anser cygnoides’ten Izole Edilen Kaz Parvovirusu’nun Tam Genomik Dizilimi

Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids

Contact Info

Product

Resources

About