Isolation of a “Helicobacter heilmanii”-like organism from the human stomach

Andersen, L. P.; Nørgaard, A.; Holck, Susanne; Blom, Jens; Elsborg, L

doi:10.1007/bf01586196

Cited by 57 publications

(46 citation statements)

References 7 publications

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“…So far, very few laboratories have succeeded in the isolation of H. heilmannii s.l. from the gastric mucosa of cats, dogs, or pigs [15,44,45,67] and only H. bizzozeronii and H. felis have been isolated from the human gastric mucosa [35,[68][69][70] . Currently, the most accurate method available for conclusive species identification is the use of PCR, followed by sequencing of specific target genes.…”

Section: Diagnosismentioning

confidence: 99%

Helicobacter heilmannii sensu lato: An overview of the infection in humans

Bento-Miranda

Figueiredo

2014

WJG

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Helicobacter heilmannii sensu lato (H. heilmannii s.l.) is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other. H. heilmannii s.l. infect the stomach of several animals and may have zoonotic potential. Although the prevalence of these infections in humans is low, they are associated with gastric pathology, including mucosa-associated lymphoid tissue lymphoma, making them a significant health issue. Here, the taxonomy, epidemiology, microbiology, diagnosis, and treatment of these infections will be reviewed. The gastric pathology associated with H. heilmannii s.l. infections in humans will also be addressed. Finally, the features of the complete bacterial genomes available and studies on species-specific pathogenesis will be reviewed. The understanding of the mechanisms that underlie gastric disease development mediated by the different bacterial species that constitute H. heilmannii s.l. is essential for developing strategies for prevention and treatment of these infections. Core tip: Helicobacter heilmannii sensu lato is a group of non-Helicobacter pylori Helicobacter species that infect the stomach of animals and humans. In the human stomach, these infections are associated with several pathologies, but it is currently unknown whether certain species are more often associated with a certain disease outcome than others. The access to bacterial genomes together with the availability of increasing numbers of in vitro isolates will allow significant advances in the understanding of species-specific bacteria-host interactions in disease pathogenesis and will be essential for future development of strategies to prevent and treat these infections.

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Section: Diagnosismentioning

confidence: 99%

Helicobacter heilmannii sensu lato: An overview of the infection in humans

Bento-Miranda

Figueiredo

2014

WJG

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“…"Candidatus Helicobacter heilmannii" has not been reliably cultured to date, but it can be maintained in mouse stomachs (2,23). In humans, "Candidatus Helicobacter heilmannii" infection has been associated with minimal gastritis, occasional gastroduodenal ulcers, and mucosa-associated lymphoid tissue (MALT)-type gastric B-cell lymphomas (MALT lymphomas) (15).…”

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confidence: 99%

“ Candidatus Helicobacter heilmannii” from a Cynomolgus Monkey Induces Gastric Mucosa-Associated Lymphoid Tissue Lymphomas in C57BL/6 Mice

et al. 2007

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Both Helicobacter pylori and "Candidatus Helicobacter heilmannii" infections are associated with peptic ulcers, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphomas. However, good animal models of H. pylori clinical diseases are rare. In this study, we aimed to establish an animal model of "Candidatus Helicobacter heilmannii" gastric MALT lymphoma. We used a urease-positive gastric mucosal and mucus homogenate from a cynomolgus monkey maintained in C57BL/6 mouse stomachs. The bacterium in the homogenate was identified as "Candidatus Helicobacter heilmannii" based on a DNA sequence analysis of the 16S rRNA and urease genes. Mucosal and mucus homogenates were used to inoculate C57BL/6 mice, which were then examined for 24 months. We observed a gradual increase in the surface area of protrusive lesions in almost all infected C57BL/6 mouse fundic stomachs 6 months after infection. Light microscopic observations revealed an accumulation of B lymphocytes along with destruction of glandular elements and the presence of lymphoepithelial lesions consistent with low-grade MALT lymphomas. Electron microscopic observation revealed numerous "Candidatus Helicobacter heilmannii" bacilli in the fundic glandular lumen, the intracellular canaliculi, and the cytoplasm of intact cells, as well as damaged parietal cells. In conclusion, "Candidatus Helicobacter heilmannii" induced gastric MALT lymphomas in almost 100% of infected C57BL/6 mice after a 6-month period associated with the destruction of parietal cells.

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“…Helicobacter heilmannii is the name proposed for a 4-to 10-m-long, spiral-shaped, motile bacterium with three to eight coils, a wavelength of about 1 m, up to 14 uni-or bipolar flagella, and no periplasmic filaments (1,24) that is found in the stomachs of 0.2 to 4% of patients with gastritis (1,2,7,27). The bacterium was first described as "Gastrospirillium hominis" but was reclassified following 16S ribosomal DNA (rDNA) sequencing (3,22,23) as "H.…”

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confidence: 99%

“…Definitive culture of "H. heilmannii" has not been achieved to date (2) and diagnosis is usually made on the basis of its distinct spiral morphology, compared with H. pylori, on silverstained tissue sections. However, as a variety of large gastric spiral organisms such as H. felis, H. salomonis, and H. bizzozeronii are indistinguishable from "H. heilmannii" on routine light microscopy, and as H. pylori grown in a broth culture can adopt a morphology identical to that of "H. heilmannii" (5), genetic techniques such as PCR, sequence analysis of cloned and uncloned PCR products and fluorescence in situ hybridization (FISH) with specific probes are required for more definitive identification (28).…”

mentioning

confidence: 99%

Evaluation of “Helicobacter heilmannii” Subtypes in the Gastric Mucosas of Cats and Dogs

Priestnall¹,

Wiinberg²,

Spohr³

et al. 2004

J Clin Microbiol

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Infection with candidatus "Helicobacter heilmannii" is associated with gastritis and mucosa-associated lymphoid tissue lymphoma in people. Infection with "H. heilmannii" type 1 predominates (80%) and is thought to be acquired from dogs, cats, or pigs. We further examined the zoonotic potential of dogs and cats by amplifying gastric DNA from cats (n ‫؍‬ 45) and dogs (n ‫؍‬ 10) with primers against "H. heilmannii" ureB and 16S rRNA genes and sequencing the products. Fluorescence in situ hybridization (FISH) with eubacterial and "H. heilmannii"-specific probes was employed to directly visualize "H. heilmannii" types and their intragastric distribution. ureB sequences of "H. heilmannii" amplicons clustered with human and feline isolates of "H. heilmannii" and were distinct from the "H. heilmannii"-like organisms (HHLO) H. felis, H. salomonis, and H. bizzozeronii. 16S ribosomal DNA sequences in 20 "H. heilmannii"-infected cats and dogs were distinct from "H. heilmannii" type 1 and "H. suis" and clustered with "H. heilmannii" types 2 and 4. FISH confirmed the presence of "H. heilmannii" types 2 and 4 in dogs but failed to definitively characterize the "H. heilmannii" types present in cats. In infected dogs, "H. heilmannii" inhabited the gastric mucus and glands, and in dogs coinfected with other HHLO it shared the same gastric niche. The results indicate that dogs and cats are predominantly colonized by "H. heilmannii" bacteria that are distinct from type 1 and from "H. suis." As "H. heilmannii" type 1 predominates in people, the zoonotic risk posed by dogs and cats is likely small.Helicobacter heilmannii is the name proposed for a 4-to 10-m-long, spiral-shaped, motile bacterium with three to eight coils, a wavelength of about 1 m, up to 14 uni-or bipolar flagella, and no periplasmic filaments (1, 24) that is found in the stomachs of 0.2 to 4% of patients with gastritis

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Isolation of a “Helicobacter heilmanii”-like organism from the human stomach

Cited by 57 publications

References 7 publications

Helicobacter heilmannii sensu lato: An overview of the infection in humans

Helicobacter heilmannii sensu lato: An overview of the infection in humans

“ Candidatus Helicobacter heilmannii” from a Cynomolgus Monkey Induces Gastric Mucosa-Associated Lymphoid Tissue Lymphomas in C57BL/6 Mice

Evaluation of “Helicobacter heilmannii” Subtypes in the Gastric Mucosas of Cats and Dogs

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