Isolation of a Lactococcus lactis Strain Producing
                    Anti-staphylococcal Bacteriocin

Yang, Jung-Mo; Moon, Gi-Seong

doi:10.5851/kosfa.2018.e67

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…Furthermore, the TBC count increased with the increased ratio of LAB. These results were supported by previous findings of (Park et al, 2018; Yang and Moon, 2018) reported that administration of LAB in minced beef inhibiting the growth of bacteria.…”

Section: Resultssupporting

confidence: 92%

Nitroso-hemoglobin Increased the Color Stability and Inhibited the Pathogenic Bacteria in a Minced Beef Model: A Combined Low-field NMR Study

Hhm

Jin

et al. 2019

Food Sci Anim Resour

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This study mainly investigated the improvement effect of nitroso-hemoglobin (NO-Hb) and four lactic acid bacteria (LAB) (Streptococcus thermophiles, Lactobacillus bulgaricus, Lactobacillus casei, and Montessori enterococcus) on the color and microbiological qualities of raw beef. Three strains of Escherichia coli, Staphylococcus, Salmonella, and Pseudomonas were used as pathogenic bacteria. The results showed that both NO-Hb and LAB could enhance the color stability and scavenged the spoilage bacteria in a minced beef model. But the improvement effect of NO-Hb was more significant than LAB. This suggested that NO-Hb, as a novel ingredient, could be used as a promising substitute for nitrite in meat products to improve the color and safety of meat products. In addition, low field (LF)-NMR method has been established to be practicable to identify changes in the relaxation times of water and fat caused by different type of bacteria and the storage periods. The number of relaxation components in minced beef was affected by bacteria and increase of the storage period.

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Section: Resultssupporting

confidence: 92%

Nitroso-hemoglobin Increased the Color Stability and Inhibited the Pathogenic Bacteria in a Minced Beef Model: A Combined Low-field NMR Study

Hhm

Jin

et al. 2019

Food Sci Anim Resour

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“…Furthermore, L. lactis has been found to change the virulence of S. aureus by downregulating its accessory gene regulator (agr) system (49). L. lactis has also been found to produce S. aureus inhibiting biocins (50). Additional inhibiting methods might consist of a quorum-quenching effect, as it has been shown between Bacillus subtilis and S. aureus in humans (51).…”

Section: Discussionmentioning

confidence: 99%

Targeted isolation of bacteria with potential to competitively excludeStaphylococcus aureusin the upper respiratory tract of pigs

Vlasblom,

Patel,

Lawlor

et al. 2024

Preprint

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Considering global antimicrobial resistance (AMR) prevalence, alternative or complementary strategies to antimicrobial use, are of interest. Livestock-associated methicillin-resistantStaphylococcus aureus(LA-MRSA) is of particular interest as despite significant AMU reduction, LA-MRSA prevalence in pig husbandry has not decreased. We performed targeted isolation of bacterial species with potential antagonism against LA-MRSA in pig farms. Duplicate piglet nasal swabs from three European countries (Germany, Ireland and The Netherlands) were taken longitudinally from birth up to 10 weeks, one for amplicon sequencing and qPCR, and the other was cryopreserved for culturing. We identified potential probiotic species by anticorrelation analysis of bacterial abundance from amplicon sequencing data with quantitativeS. aureusestimates from qPCR data from the samples. A literature-screen was performed on the species identified, to determine their probiotic potential. Following this, 1302 isolates were grown from selected cryopreserved swabs and identified using MALDI-TOF and additional 16S rRNA gene sequencing to isolate the anticorrelating species. Ninety-five isolates of interest were screened for absence of tetracycline resistance and hemolytic activity and whole genome sequencing was conducted to verify their taxonomy and to assess their AMR and virulence gene profile. Additional phenotypic antimicrobial resistance testing selected three differentLactococcus lactisstrains. During anin vitrochallenge using spent medium, all three strains demonstrated inhibition against twoS. aureusstrains. TheseL. lactisstrains may have the ability to be used safely to reduce LA-MRSA carriage in the nasal passages of pigs but furtherin vivotesting is necessary to confirm this potential.ImportanceAn approach to tackle antimicrobial resistance in livestock is to use competitive exclusion agents. We employed a sequencing guided isolation approach to identify bacterial species within silicoantagonism to livestock-associatedStaphylococcus aureus(LA-MRSA) in the nasal microbiome of piglets. Based on this, threeLactococcus lactisisolates were found to be suitable for further probiotic testing. This strategy can be used to effectively identify, isolate, and screen bacteria that live in antagonism with opportunistic pathogens such as LA-MRSA in a complex microbiome.

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“…Previously, 73 strains of S. aureus , including MRSA strains, were found to be susceptible against recombinant SAP8 endolysin, including S. aureus KCTC 3881, which demonstrated that recombinant SAP8 is not strain-specific [ 16 ]. In our previous study, we tested crude nisin from L. lactis strain against several S. aureus strains and found strongest activity of nisin against S. aureus KCTC 3881, chosen as a model strain in this study [ 19 ]. To test activities of recombinant SAP8 endolysin and nisin, S. aureus KCTC 3881 strain was cultured in MRS broth (BD, Sparks, MD, USA) at 37 ℃ for 12 h. To measure CFU/mL, 10 times serial dilutions of culture was made, then we poured 10 µL of each dilution on MRS agar plate in triplicate form.…”

Section: Methodsmentioning

confidence: 99%

Combination Treatment for Inhibition of the Growth of Staphylococcus aureus with Recombinant SAP8 Endolysin and Nisin

et al. 2022

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Staphylococcus aureus, a pathogenic species of genus Staphylococcus involved in foodborne illness always remain among the top priorities of the world major concerns. In the present study, we have used recombinant SAP8 endolysin from the bacteriophage SAP8 and commercial nisin to inhibit the viability of pathogenic S. aureus KCTC 3881 cells; however, the approach was not identified as cost-effective. A gradual decrease in the viable S. aureus KCTC 3881 cell counts was observed with an increase in the concentrations of recombinant SAP8 endolysin and nisin. However, combined treatment with recombinant SAP8 endolysin and nisin decreased the viable S. aureus KCTC 3881 cell counts in a significant manner. The combination of 0.01 µM of recombinant SAP8 endolysin with 9 IU/mL and 18 IU/mL of nisin demonstrated a promising decrease in the viable cell counts of the strain. Under the scanning electron microscope, the combination treatment with 0.01 µM of recombinant SAP8 endolysin and 18 IU/mL of nisin showed complete cellular destruction of S. aureus KCTC 3881. We propose that a combination of recombinant SAP8 endolysin and nisin could be a strong alternative to antibiotics to control the growth of S. aureus including MRSA.

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Isolation of a Lactococcus lactis Strain Producing Anti-staphylococcal Bacteriocin

Cited by 8 publications

References 19 publications

Nitroso-hemoglobin Increased the Color Stability and Inhibited the Pathogenic Bacteria in a Minced Beef Model: A Combined Low-field NMR Study

Nitroso-hemoglobin Increased the Color Stability and Inhibited the Pathogenic Bacteria in a Minced Beef Model: A Combined Low-field NMR Study

Targeted isolation of bacteria with potential to competitively excludeStaphylococcus aureusin the upper respiratory tract of pigs

Combination Treatment for Inhibition of the Growth of Staphylococcus aureus with Recombinant SAP8 Endolysin and Nisin

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