Isolation of a leukovirus from a continuous human cell line

Zhdanov, V. M.; Soloviev, V. D.; Bektemirov, T. A.; Filatov, Felix; Bykovsky, A. F.

doi:10.1007/bf01241009

Cited by 13 publications

(6 citation statements)

References 6 publications

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“…MPV and all other viruses used were purified from culture super natant fluids by equilibrium density gradient centri fugation and were concentrated as previously described [20], X-381 virus was purified from the supernatant fluid of X-381 cells established from a rhesus lactating mammary gland (3], LV was grown in bat lung cells (supplied by Dr. Todaro) [7], SMRV grown in canine Cf2Th cells cocultivated with squirrel monkey lung cells was supplied by Dr. R. Heberling and Dr.S.Kalter [6], Type-D retroviruses were purified from superna tant fluids of HeLa cells [10][11][12], from a cell line derived from a human amniotic cell, AO [13], and from two cell lines derived from human leukemic cells, T9 [14] and J96 [15]. Simian sarcoma virus I grown in NC-37 cells, baboon virus grown in BKCT (baboon kidney cocultivated with canine thymus) cells, and mouse mammary tumor virus grown in murine Mm5mt cells were obtained through the Biological Carcinogenesis Branch of the National Cancer Institute.…”

Section: Virus and Cellsmentioning

confidence: 99%

“…Viruses that are morphologically related to MPV have been identified in several human tissue culture cell lines, including HeLa cells [10][11][12] and cell lines established from amniotic cells (AO [13]) and leukemic cells (T9 [14]; J96 [15]). The AO, T9 and J96 cell lines were shown to have a type-A glucose-6-phosphate dehydrogenase isoenzyme and marker chro mosomes in common with HeLa cells [16].…”

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confidence: 99%

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Immunological Characterization of Type-D Retrovirus Isolates from Human Cells

Colcher¹

1980

Intervirology

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Competition radioimmunoassays for the 27,000 molecular weight (p27) major internal protein and the low-molecular-weight 10,000–12,000 (p10–12) polypeptides of the primate type-D retroviruses, Mason-Pfizer virus (MPV) and langur virus (LV), were established. Type-D isolates from various primate and human tumor cells were examined in both these assays. There were no detectable immunological differences between MPV and two type-D retroviruses previously reported to be isolated from human cell lines (HeLa and AO). Type-D retroviruses isolated from human T9 and J96 cells (cell lines derived from human leukemic cells), however, were clearly distinguishable from both MPV and LV in their respective p10–12 radioimmunoassays. These assays thus demonstrate that type-D retroviruses isolated from some human cells may differ immunologically from morphologically similar viruses isolated from nonhuman primates.

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Section: Virus and Cellsmentioning

confidence: 99%

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confidence: 99%

Immunological Characterization of Type-D Retrovirus Isolates from Human Cells

Colcher¹

1980

Intervirology

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“…Since completion of this work, Russian authors have published several papers o n the characterization of '' oncornaviruses from continuous human cell cultures" (Zhdanov et al, 1972;Zhdanov et al, 1973). Most of the particles observed in HeLa and other cells were grouped as B-type, and only some as C-type particles.…”

Section: Figurementioning

confidence: 99%

Detection of oncornavirus‐like particles in HeLa cells. I. Fine structure and comparative morphological classification

Gelderblom

Bauer

Ogura

et al. 1974

Intl Journal of Cancer

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Oncornavirus‐like particles have been consistently observed over a period of 3 years in HeLa cells obtained from several laboratories. The particles mature by budding, in that previously fully assembled A‐type‐like particles are enveloped by the protruding cell membrane with subsequent release of the virion. When compared morphologically to B‐ and C‐type particles, distinct differences were observed; however, the HeLa particles closely resemble two primate viruses, namely the Mason‐Pfizer monkey virus and a recently discovered virus derived from human brain cells. HeLa particles demonstrate a narrow host range. Thus a new morphological group of viruses emerges that might be specific for primates.

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“…Virus particles with morphological similarities to RNA viruses possessing RNA-instructed DNA polymerase (retraviruses) have been reported in transformed human cell lines (1,8,16,12,17,32,33; H. Bauer, personal communication). Despite morphological similarities by electron microscopy, the usual appearance of these virus-like particles did not entirely satisfy the criteria which define the ultrastructural appearance of established type-C or type-B retravirus particles (6,10).…”

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confidence: 99%

Mason-Pfizer Virus Characterization: a Similar Virus in a Human Amniotic Cell Line

Parks

Gilden

Bykovsky

et al. 1973

J Virol

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Mason-Pfizer monkey virus (MP-MV) is a RNA virus with an RNA-instructed DNA polymerase first isolated from a rhesus monkey mammary adenocarcinoma in 1970. Until recently, there have been no other isolates. A continuous human amnion cell line, AO, was found to be producing a virus indistinguishable or closely related to the Mason-Pfizer virus as measured by morphological, immunological, and biochemical methods. By thin-section electron microscopy, the extracellular virus particle in AO line is 115 to 130 nm in diameter and has a preformed nucleoid (80 to 90 nm) before budding, properties which are also characteristic of MP-MV. Two proteins of the virus from the AO line were studied. By immunodiffusion, sera which react specifically with MP-MV give a line of identity with virus from the AO line. The AO viral RNA-instructed DNA polymerase purified by phosphocellulose chromatography was specifically inhibited by anti-MP-MV polymerase sera, and the AO cells contained both DNA and RNA sequences related to MP-MV 'H-DNA. Viruses thus far indistinguishable from MP-MV have also recently been found by others in different human lines, raising again the question of the species of origin of MP-MV. Because the virus in the AO cells cannot be differentiated from MP-MV, we attempted to determine the origin of MP-MV virus by measuring DNA sequences related to MP-MV 3H-DNA in uninfected human and rhesus monkey cells. The quantity of MP-MV-like DNA sequences in uninfected primate tissues was found to be much lower than the amount of DNA sequences of murine type-B or type-C viruses in uninfected murine tissues. Thus, it was not possible to determine whether the virus produced by AO cells or MP-MV was of human or monkey origin, or both.

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Isolation of a leukovirus from a continuous human cell line

Cited by 13 publications

References 6 publications

Immunological Characterization of Type-D Retrovirus Isolates from Human Cells

Immunological Characterization of Type-D Retrovirus Isolates from Human Cells

Detection of oncornavirus‐like particles in HeLa cells. I. Fine structure and comparative morphological classification

Mason-Pfizer Virus Characterization: a Similar Virus in a Human Amniotic Cell Line

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