Isolation of a Nonylphenol-degrading Microbial Consortium

Song, Won Yeong; Lim, Keun-Sick; Yu, Daeung; Park, Mi-Eun; Jeong, Eun-Young; Kim, Dong‐Myung; Chung, Yong-Ho; Kim, Young‐Mog

doi:10.5657/kfas.2011.0325

Cited by 2 publications

(5 citation statements)

References 28 publications

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“…The extension of the degradation process has been reported as temperature dependent [6, 42]. In this study degradation was close to 65% at 27°C; similar values have been reported by other authors [6, 40, 41, 43]. …”

Section: Resultssupporting

confidence: 90%

“…Finally, a mixture culture of Yas1/Yas2 in 1% NPEO 15 medium, pH 8, showed a synergistic effect on the bacterial growth, reaching maximal values (0.095 au) at 9 h incubations and 27°C, which represents a stimulatory average factor close to 5.7 with respect to the individual bacterial cultures at 1% NPEO 15 medium (0.014 and 0.02 au); additionally, the lag phase was absent (see Figure 4). This stimulatory effect is probably due to NPEO x cometabolism by the Yas1/Yas2 system, as it has been described for other bacterial consortiums [41]. …”

Section: Resultsmentioning

confidence: 59%

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Biodegradation of Polyethoxylated Nonylphenols

et al. 2013

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Polyethoxylated nonylphenols, with different ethoxylation degrees (NPEOx), are incorporated into many commercial and industrial products such as detergents, domestic disinfectants, emulsifiers, cosmetics, and pesticides. However, the toxic effects exerted by their degradation products, which are persistent in natural environments, have been demonstrated in several animal and invertebrate aquatic species. Therefore, it seems appropriate to look for indigenous bacteria capable of degrading native NPEOx and its derivatives. In this paper, the isolation of five bacterial strains, capable of using NPEO15, as unique carbon source, is described. The most efficient NPEO15 degrader bacterial strains were identified as Pseudomonas fluorescens (strain Yas2) and Klebsiella pneumoniae (strain Yas1). Maximal growth rates were reached at pH 8, 27°C in a 5% NPEO15 medium. The NPEO15 degradation extension, followed by viscometry assays, reached 65% after 54.5 h and 134 h incubation times, while the COD values decreased by 95% and 85% after 24 h for the Yas1 and Yas2 systems, respectively. The BOD was reduced by 99% and 99.9% levels in 24 h and 48 h incubations. The viscosity data indicated that the NPEO15 biodegradation by Yas2 follows first-order kinetics. Kinetic rate constant (k) and half life time (τ) for this biotransformation were estimated to be 0.0072 h−1 and 96.3 h, respectively.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 59%

Biodegradation of Polyethoxylated Nonylphenols

et al. 2013

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“…No reports have been published regarding NP bioremediation in aquatic environments. Therefore, this study isolated NP-degrading bacteria from the microbial consortium SW-3, which was previously isolated from an aqueous environment (Song et al, 2011). We also performed a kinetic analysis of NP degradation by individual and groups of bacterial strains.…”

Section: Resultsmentioning

confidence: 99%

“…To isolate NP-degrading bacteria from the NP-degrading microbial consortium SW-3 (Song et al, 2011), the consortium strain was cultivated in YNB medium containing 100 ppm NP at 25°C under aerobic conditions. 100 uL of the culture was taken at intervals and spread on YNB agar plates containing 100 ppm NP.…”

Section: Isolation and Culture Of Np-degrading Bacteriamentioning

confidence: 99%

“…A biological decomposition method by microorganisms, called bioremediation, has been considered an environmentally favorable method to restore environments contaminated with harmful non-resolvable chemicals (Kim et al, 2004;Lee et al, 2009;Song et al, 2011). Bioremediation decomposes organic toxic substances into water and carbon dioxide (Ripp et al, 2000;Kang and Kim, 2007;Kim et al, 2007;Lee et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Nonylphenol-degrading Bacteria

Yu¹,

Kim²,

Chung³

et al. 2012

Fisheries and aquatic sciences

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To isolate a nonylphenol (NP)-degrading bacterium, we isolated a single colony from the NP-degrading microbial consortium SW-3, which was previously isolated from an aqueous environment. Ten colonies that exhibited different cell morphologies were isolated and the strains were named SW- 3-A, -B, -C, -D, -E, -F1, -F2, -G, -H, and -I. The ability of isolates to degrade NP was evaluated by kinetic analysis by the constant of NP degradation rate (k 1 ) and the half-life time of NP degradation (t 1/2 ). SW-3-F1, -F2, -G, and -I strains were superior at degrading NP. The k 1 and t 1/2 values of the four strains were sixfold higher and one-sixth lower, respectively, than those of the consortium strain. Additionally, SW-3-F1, -G, and -I strains were tested for their ability to degrade NP during coculture. NP degradation by coculture with a combination of all three strains was inferior to that of culture conducted with single isolates, suggesting that the three strains are antagonistic toward each other during NP degradation.

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Isolation of a Nonylphenol-degrading Microbial Consortium

Cited by 2 publications

References 28 publications

Biodegradation of Polyethoxylated Nonylphenols

Biodegradation of Polyethoxylated Nonylphenols

Isolation and Characterization of Nonylphenol-degrading Bacteria

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