Isolation of a Novel Alkaline Lipase Producing Fungus Aspergillus fumigatus MTCC 9657 from Aged and Crude Rice Bran Oil and Quantification by HPTLC

Rajan, A. R.; Kumar, Dalip; Nair, Anusha

doi:10.3923/ijbc.2011.116.126

Cited by 24 publications

(20 citation statements)

References 16 publications

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“…The formation of opalescence surrounding the fungal colony was recorded as positive for lipase production (Rajan et al, 2011).…”

Section: Screening For Lipase Productionmentioning

confidence: 99%

Isolation and Screening of Industrially Important Fungi from the Soils of Western Ghats of Agumbe and Koppa, Karnataka, India

Mukunda¹,

R²,

Prashith³

2013

Sci. Technol. Arts Res. J.

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Article InformationThe present study is an attempt to isolate the fungi from the unexplored soils of Western Ghats and screen them for the production of some very important hydrolytic enzymes such as amylase, cellulose, CMCase, protease, lipase and pigment production. In this work, more than 200 fungal isolates were isolated from the forest soil were screened for the production of extracellular enzymes, identification of the screened isolates and secondary screening for amylase production was done. A total of 167 isolates were characterized, in them some up to species level. Aspergillus, Penicillium, Trichoderma and Cladosporium were predominated. A total of 14 isolates were selected for the secondary screening for amylase production. Aspergillus isolate 199 and Aspergillus isolate 43 produced more protein (169±07 and 160±04µg) respectively. Amylase assay also revealed greater activities (4.98±0.06 and 4.93± 0.07µ moles/ ml) in Aspergillus isolate 199 and Aspergillus isolate 43 respectively.

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“…The formation of opalescence surrounding the fungal colony was recorded as positive for lipase production (Rajan et al, 2011).…”

Section: Screening For Lipase Productionmentioning

confidence: 99%

Isolation and Screening of Industrially Important Fungi from the Soils of Western Ghats of Agumbe and Koppa, Karnataka, India

Mukunda¹,

R²,

Prashith³

2013

Sci. Technol. Arts Res. J.

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“…The method was also used by Vitro et al (2000) for determining the fatty acid distribution. Rajan et al (2011) quantified the liberated free fatty acids produced by the action of alkaline lipase from Aspergillus fumigates MTCC 9657 using HPTLC.…”

Section: Chromatographic Procedures Tlc/hplc/hptlc/gc-ms/lc-ms For Qumentioning

confidence: 99%

A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry

Lanka¹,

Latha²

2015

International J. of Biological Chemistry

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The increased demand for microbial originated Industrial enzymes especially lipases which have received least priority till last one and half decades and gained lots of importance in recent days owing to their applications in wide variety of fields such as food, dairy, pharmaceutical, detergent, textile, oleo-chemical, perfume and cosmetic industries etc., has lead to the identification of novel enzymes from new sources with unique properties. The present review mainly focuses on various currently available screening methods for identification of lipase producers.

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“…They also function in a non-aqueous environment and catalyze a number of useful reactions, such as esterification, transesterification, acidolysis, alcoholysis, aminolysis, and resolution of racemic mixtures [21]. Filamentous fungi are one of the best lipase producers and the preferred source for lipase [27,28].…”

Section: Discussionmentioning

confidence: 99%

Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus

Shangguan

Fan

et al. 2012

Appl Biochem Biotechnol

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A 1,080-bp cDNA (CGMCC 2873) encoding of a cold-active lipase of Aspergillus fumigatus (AFL67) was cloned and expressed in Escherichia coli for the first time. The new lipase, AFL67, was one-step purified by 8.30 folds through Ni-NTA affinity chromatography with a recovery of 86.8 %. The specific activity of purified AFL67 was 449 U mg(-1) on p-NP hexanoate. AFL67 preferentially hydrolyzed p-nitrophenyl esters of short- and medium-chain fatty acids, with p-nitrophenyl hexanoate the maximum. The optimum temperature and pH was 15 °C and 7.5, respectively. The purified AFL67 was stable at 10-25 °C for 30 min, and in the pH range of 6.0-9.0 for 16 h (at 4 °C). Its activity was increased by 47 and 50 %, in the presence of 10 % (v/v) ethanol and isopropanol, respectively. The new lipase AFL67 highly enantioselectively deacylated (S)-α-acetoxyphenylacetic acid (APA) and o-Cl-APA, m-Cl-APA, and p-Cl-APA to (S)-mandelic acid and its derivates. These features render this cold-active novel lipase AFL67 attractive for biotechnological applications in the field of enantioselective synthesis of chiral mandelic acids, o-acylated mandelic acids, and their derivates and detergent additives.

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Isolation of a Novel Alkaline Lipase Producing Fungus Aspergillus fumigatus MTCC 9657 from Aged and Crude Rice Bran Oil and Quantification by HPTLC

Cited by 24 publications

References 16 publications

Isolation and Screening of Industrially Important Fungi from the Soils of Western Ghats of Agumbe and Koppa, Karnataka, India

Isolation and Screening of Industrially Important Fungi from the Soils of Western Ghats of Agumbe and Koppa, Karnataka, India

A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry

Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus

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