Isolation of a Novel Glycoprotein from the Urine of a Patient with Chronic Myelocytic Leukemia

Abstract

A B S T R A C T Patient B. J. witlh chr-oniic myelocytic leukemia excreted 0.5-1.1 g protein per day in the urine. Gel filtration on Sephadex G-75 showed about one-third of this protein to be in molecular weight range 20,000-40,000 (fraction BJC). BJC, prepared from 9 liters of urine by gel filtration, was chromatographed on carboxymethylcellulose. Two BJC2 was found in the urine by immunoelectrophoresis in 10% of patients with neoplastic disease and was not observed in urine of other patients or in human plasma.Amino acid composition, carbohydrate content, and antigenic specificity indicate BJC1 is a previously unrecogniized member of the system of normal human

Proteins of human urine. III. Identification and two-dimensional electrophoretic map positions of some major urinary proteins.

“…For this reason, it is necessary to investigate, as sensitively as possible, both the qualitative and quantitative changes that occur in the proteins of urine under different clinical conditions. Recent findings (2)(3)(4) support earlier observations that both quantitative and qualitative alterations in urillary proteins are associated with trauma or disease.…”

The nutritional effects of cancer and its therapy

“…It is not clear whether these substances originate in tumor cells. 48 " 50 Animal models and clinical reports 36 ' 67 -69 * 70 ' 72 indicate that energy requirements of the host are increased because of acceleration of wasteful cycles 76 or because of abnormalities in the normal metabolic 52 Such reports are suggestive, but both the techniques and the assumptions used are often questionable. Because weight loss might not always be due to inadequate intake, definitive studies of the energy output of cancer patients are needed.…”