Isolation of a Polyvalent Bacteriophage for
            <i>Escherichia coli, Klebsiella pneumoniae</i>
            , and
            <i>Aerobacter aerogenes</i>

Souza, K. A.; Ginoza, Herbert S.; Haight, Roger

doi:10.1128/jvi.9.5.851-856.1972

Cited by 22 publications

(6 citation statements)

References 19 publications

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“…In this study, E. faecalis phages infected and replicated in both E. faecalis and E. faecium. Characteristics of polyvalent phages (or broad-host-range phages), which are able to infect more than one species of bacteria, have previously been observed in certain bacteriophages (Souza et al 1972;Jensen et al 1998;Hamdi et al 2017) as well as enterococcal bacteriophages (Santiago-Rodriguez et al 2013). However, these polyvalent characteristics are unlikely to affect the application of bacteriophages for microbial source tracking.…”

Section: Discussionmentioning

confidence: 92%

Comparative persistence of human sewage-specific enterococcal bacteriophages in freshwater and seawater

Booncharoen

Mongkolsuk

Sirikanchana

2018

Appl Microbiol Biotechnol

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Enterococcus faecalis bacteria have been recently reported for their ability to host bacteriophages that are specifically from human sewage, suggesting their application to track human fecal contamination in water resources. However, little is known about the survivability of sewage-specific enterococcal bacteriophages in various water matrices under ambient and storage conditions. In this study, bacteriophages that were derived from the Thailand-isolated E. faecalis strains AIM06 and SR14 exhibited morphologies consistent with the Siphoviridae, Podoviridae, and Myoviridae families. Four representative bacteriophages were separately spiked into environmental water samples (n = 7) comprising freshwater and seawater with low- and high-pollution (LF, HF, LS, and HS, respectively) levels, defined according to Thailand Water Quality Standards. All bacteriophages decayed fastest in HS or HF samples at 30 °C, reaching a 5-log reduction in 2.2 to 9.8 days, and slowest in LS samples, requiring 8.8 to 23.5 days. The decay rates were 5 to 53 times lower at a storage temperature of 5 °C. HF samples could be stored for as little as 2.5 days to prevent the decay of 50% of the phages. Myoviridae phages decayed faster than Siphoviridae phages and Podoviridae phages in most water matrices at 30 °C. Moreover, the decay rates were 1.8 to 92 times slower in filtered samples, emphasizing a strong role for water constituents, i.e., suspended solids and natural microorganisms, in phage persistence. This study emphasized that differential enterococcal bacteriophage persistence should be considered when planning the monitoring and interpreting of fecal sources by microbial source tracking.

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Section: Discussionmentioning

confidence: 92%

Comparative persistence of human sewage-specific enterococcal bacteriophages in freshwater and seawater

Booncharoen

Mongkolsuk

Sirikanchana

2018

Appl Microbiol Biotechnol

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“…pneumoniae –phage combination from the caecal effluent of a healthy woman, and the implications of our findings in relation to gastrointestinal microbial ecology and the potential of the human gut as a source of phages with therapeutic uses. Previous studies describing the isolation of lytic phages against K. pneumoniae have reported recovery of these entities from wastewater, seawater, sewage and sewage-contaminated water samples, but never directly from gastrointestinal contents ( Souza, Ginoza & Haight, 1972 ; Kumari, Harjai & Chhibber, 2009 ; Kumari, Harjai & Chhibber, 2010a ; Drulis-Kawa et al, 2011 ; Hung et al, 2011 ; Cui et al, 2012 ; Hsu et al, 2013 ; Karumidze et al, 2013 ; Jamal et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Klebsiella pneumoniaesubsp.pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman

Hoyles

Murphy

Neve

et al. 2015

PeerJ

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A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA+). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus “Kp36likevirus.”

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“…of different hosts of the same genus or between different genera are common (1,13). In dairy microbiology it is frequently observed that temperate phages for one species are able to infect other species either in a virulent or a temperate manner (11).…”

Section: Resultsmentioning

confidence: 99%

Evidence by electron micrographs for a high incidence of bacteriophage particles in the waters of Yaquina Bay, oregon: ecological and taxonomical implications

Torrella¹,

Morita²

1979

Appl Environ Microbiol

113

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A variety of viral particles, the majority of them clearly identifiable as bacteriophages, were found in the seawater of Yaquina Bay, Oregon. These phages were obtained as free particles from the seawater without employing specific hosts for enrichments or further purification in the laboratory. A variety of electron micrographs showing different morphologies of phages as well as phagebacterium interactions found in the seawater are presented. In the area where the bay received organic enrichment from seafood processing plants, a minimum of 104 phage particles per ml was estimated. Since the technique used was designed to concentrate particles 0.2 ,im in diameter or larger, it is assumed that the actual number of phage particles is higher than 104 particles per ml. The implications of the presence of such phage concentrations in bays and estuaries with a certain level of eutrophication are of obvious importance in considering the microbial ecology of these environments.

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Isolation of a Polyvalent Bacteriophage for Escherichia coli, Klebsiella pneumoniae , and Aerobacter aerogenes

Cited by 22 publications

References 19 publications

Comparative persistence of human sewage-specific enterococcal bacteriophages in freshwater and seawater

Comparative persistence of human sewage-specific enterococcal bacteriophages in freshwater and seawater

Klebsiella pneumoniaesubsp.pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman

Evidence by electron micrographs for a high incidence of bacteriophage particles in the waters of Yaquina Bay, oregon: ecological and taxonomical implications

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