A marine vibrio, designated as MP‐1, was isolated from the North Pacific Ocean and was found to be an obligate psychrophile. Growth was obtained from ‒1C (lowest tested) to 20C with optimal growth at 15–16C. The growth rate at 15C was found to be rapid under maximal aeration. Viability of the bacterium was destroyed by heating at 28.8C for 6.25 hr, and reversible damage occurred at 28.8C in 1.25 hr.
No malic dehydrogenase activity was found to occur at 101 C at various hydrostatic pressures from 1 to 700 atm. However, activity was demonstrated with hydrostatic pressures above 700 atm, with optimal activity at 1,300 atm at the same temperature. Explanation of the data is based upon thermal denaturation of the enzyme, which involves a molecular volume increase of the enzyme. The molecular volume increase is counteracted by hydrostatic pressure. Pressures above 700 atm to 1,500 atm (highest employed) were sufficient to offset the denaturation by 101 C which probably resulted in an incomplete denaturation of malic dehydrogenase.
Leakage of various cellular components into the surrounding menstruum occurred when Vibrio marinus was subjected to temperatures above 20 C (organism's maximal growth temperature). These materials, listed in decreasing rates of leakage, were identified as protein, deoxyribonucleic acid, ribonucleic acid, and amino acids. The amount of polar amino acids increased as the time and temperature of heat treatment were increased, whereas the nonpolar amino acids decreased. The ribonucleic acid in the supernatant fluid resulting from heat treatment was both polymeric and nonpolymeric. Leakage of cellular components may be one of the reasons that V. marinus MP-1 loses viability when exposed to temperatures above its maximal temperature for growth.
O VER THE PAST twenty years or so various essays have appeared which deal with Spirit Christology. 1 The purpose of this study is to summarize the ground that has been gained, and at the same time to make the case for Spirit language in Christology. By a Spirit Christology I mean one that "explains" how God is present and active in Jesus, and thus Jesus' divinity, by using the biblical symbol of God as Spirit, and not the symbol Logos. It is the contention of those who propose such a thoroughgoing Spirit Christology that it expresses in a more adequate way for our time what has been expressed through a Logos Christology. 2 We can begin the discussion by describing the situation in which Christology is being written today as one that makes Spirit Christology attractive. Ours is an historically conscious period, and a Christology that does not betray an historical consciousness as its presupposition will not be credible. As a consequence of this historical consciousness, Christology today begins overwhelmingly with a consideration of Jesus and proceeds throughout to underline and even stress the humanity of Jesus. Clearly this stands in reaction against an overly abstract portrayal of the identity and status of Jesus. Positively this point of departure relies on the data of the Synoptic Gospels in which Jesus is portrayed as a human being who was related to God, 1 Some essays that may serve as an introduction to Spirit Christology are the following: James D. G. Dunn, Jesus and the Spirit (London: SCM, 1975); idem, Christology in the Making (Philadelphia: Westminster, 1980); Olaf Hansen, "Spirit Christology: A Way out of Our Dilemma?" in The Holy Spirit in the Life of the Church, ed. P. Opsahl (Minneapolis: Augsburg, 1978) 172-203; Norman Hook, "A Spirit Christology," Theology 75 (1972) 226-32; Harold Hunter, "Spirit Christology: Dilemma and Promise," Heythrop Journal 24 (1983) 127-40, 266-77 (this essay is hostile to Spirit Christology on the basis of a reading of scriptural and early Christian sources); G. W. H. Lampe, "The Holy Spirit and the Person of Christ," in Christ, Faith and History, ed. S. W. Sykes and J. P. Clayton (Cambridge: Cambridge Univ., 1972) 111-30; idem, God as Spirit (Oxford: Clarendon, 1977); Paul W. Newman, A Spirit Christology: Recovering the Biblical Paradigm of Christian Faith (Lanham, Maryland: Univ. Press of America, 1987); Philip J. Rosato, "Spirit Christology: Ambiguity and Promise," TS 38 (1977) 423-49. 2 Rosato's essay cited in the last note does not propose a thoroughgoing Spirit Christology but seeks to integrate themes from this Christology into the classical Christological and trinitarian frameworks. A summary of the problems involved in Logos Christology can be found in Newman, A Spirit Christology 1-27. 257 at Harvard Libraries on June 29, 2015 tsj.sagepub.com Downloaded from
A lytic bacteriophage isolated from sewage was found to attack strains of Aerobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae, but not members of the genera Salmonella, Proteus, and Serratia. The phage, designated rmp, contained deoxyribonucleic acid with a 50%"c guanine plus cytosine ratio and a molecular weight of 23.1 X 101 daltons. Single-step growth experiments of rmp plated at 37 C on A. aerogenes A2 gave a mean latent period of 20 min, an average burst size of 103 plaque-forming units/infected cell, and an average adsorption rate
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