Isolation of a Pseudomonas monteilli strain with a novel phosphotriesterase

Horne, Irene

doi:10.1016/s0378-1097(01)00518-3

Cited by 13 publications

(17 citation statements)

References 23 publications

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“…These include Flavobacterium sp. [50], Nocardiodes simplex [51], Pseudomonas monteilli [52] and a microbial consortia [53]. There is still limited reporting on bacteria with dual action that are able to detoxify both xenobiotics and heavy metals at the same time.…”

Section: Growth Of Enterobacter Sp Strain Saw-1 On Pesticidesmentioning

confidence: 99%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Abstract. One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 ℃, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, dadonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted.

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Section: Growth Of Enterobacter Sp Strain Saw-1 On Pesticidesmentioning

confidence: 99%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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“…Pseudomonas monteilli was isolated which can hydrolyze coumaphos as well as its oxo analogue coroxon but it can utilize only coroxon as a sole source of phosphorus, not coumaphos or its hydrolysis product DETP. This bacterium degrades coumaphos and diazinon but not parathion (Horne et al , 2002a). Coumaphos is degraded by the other microorganisms like Flavobacterium sp.…”

Section: Microbial Degradation Of Organophosphorus Compoundsmentioning

confidence: 99%

Microbial degradation of organophosphorus compounds

Singh¹,

Walker²

2006

FEMS Microbiol Rev

987

524

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Synthetic organophosphorus compounds are used as pesticides, plasticizers, air fuel ingredients and chemical warfare agents. Organophosphorus compounds are the most widely used insecticides, accounting for an estimated 34% of world-wide insecticide sales. Contamination of soil from pesticides as a result of their bulk handling at the farmyard or following application in the field or accidental release may lead occasionally to contamination of surface and ground water. Several reports suggest that a wide range of water and terrestrial ecosystems may be contaminated with organophosphorus compounds. These compounds possess high mammalian toxicity and it is therefore essential to remove them from the environments. In addition, about 200,000 metric tons of nerve (chemical warfare) agents have to be destroyed world-wide under Chemical Weapons Convention (1993). Bioremediation can offer an efficient and cheap option for decontamination of polluted ecosystems and destruction of nerve agents. The first micro-organism that could degrade organophosphorus compounds was isolated in 1973 and identified as Flavobacterium sp. Since then several bacterial and a few fungal species have been isolated which can degrade a wide range of organophosphorus compounds in liquid cultures and soil systems. The biochemistry of organophosphorus compound degradation by most of the bacteria seems to be identical, in which a structurally similar enzyme called organophosphate hydrolase or phosphotriesterase catalyzes the first step of the degradation. organophosphate hydrolase encoding gene opd (organophosphate degrading) gene has been isolated from geographically different regions and taxonomically different species. This gene has been sequenced, cloned in different organisms, and altered for better activity and stability. Recently, genes with similar function but different sequences have also been isolated and characterized. Engineered microorganisms have been tested for their ability to degrade different organophosphorus pollutants, including nerve agents. In this article, we review and propose pathways for degradation of some organophosphorus compounds by microorganisms. Isolation, characterization, utilization and manipulation of the major detoxifying enzymes and the molecular basis of degradation are discussed. The major achievements and technological advancements towards bioremediation of organophosphorus compounds, limitations of available technologies and future challenge are also discussed.

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“…This property can be accelerated by applying genetic engineering techniques. The unique Singh and Walker, 2006;Serdar et al, 1982.Somara andSiddavattam, 1995;Horne et al, 2002;Cheng et al, 1996;Cheng et al, 1997;Zhongli et al, 2001;Zhang et al, 2005;Chen and Mulchandani (1998);Chen et al, 1999;Amitai et al, 1998;Liu et al, 2001;Liu et al, 2004;Liu et al, 2001 Agrobacterium radiobacter Alteromonas sp. …”

Section: Role Of Catabolic Genes and Genetic Engineering For Pesticidmentioning

confidence: 99%