Isolation of a strain of Trichoderma reesei with improved glucoamylase secretion by flow cytometric sorting

Throndset, William; Bower, B.; Caguiat, Rodante; Baldwin, Toby; Ward, Mick

doi:10.1016/j.enzmictec.2010.09.005

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…The highly efficient cellulase producer was originally derived from the wild type strain T. reesei QM6a by applying a genetic engineering approach. [8] Nevertheless, the gene interactions in these mutants are still unknown, [36,51] probably due to the complexity of several transcription factors that govern the modifications. Of course, for improvement of the quantity and the quality of the obtained enzyme, all of those factors as well as the side effects of them should be determined.…”

Section: Cellulase Production By T Reeseimentioning

confidence: 99%

“…[7] Therefore, researchers have made many efforts to develop a map of genomes for its manipulation, and identification of the sequences of the corresponding genomes from the wild type strains such as T. reesei QM6a and many other mutants. [8] T. reesei has been studied for more than five decades due to its potential applications in bio-industries.…”

Section: Introductionmentioning

confidence: 99%

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Trichoderma reesei, a superior cellulase source for industrial applications

Keshavarz

Khalesi

2016

Biofuels

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Trichoderma reesei, a superior cellulase source for industrial applications, is a genus of filamentous fungi with an exceptional potential to produce various enzymes and proteins including cellulases, hemicellulases, and hydrophobins. Growing on easy-access low-cost substrates, as well as a broad range of products produced by T. reesei, represent this genus as the first option for bio-industries to produce required enzymes and proteins with an efficient direction of bioprocess. Recently, many researchers have made efforts to improve the yield of T. reesei cellulases using recombinant strains, optimization of the substrates, and applying the synergistic effects of co-cultivation using different microorganisms. The current findings may help industries such as biofuel producers to reduce the cost of cellulase production by up to 40%. In this review, recent advances in last five years regarding the improvement in cellulase productivity using T. reesei have been pointed out and the highlights have been summarized.

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Section: Cellulase Production By T Reeseimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trichoderma reesei, a superior cellulase source for industrial applications

Keshavarz

Khalesi

2016

Biofuels

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“…The study reported that FACS isolated recombinant T. reesei strain produced up to 2 mg hexadecanol per gram of biomass. A study by Throndset et al . reported enhanced secretion of industrially important enzyme glucoamylase in T. reesei strain, improved by flow cytometric sorting.…”

Section: Applications Of Flow Cytometry In Oleaginous Microorganismsmentioning

confidence: 99%

“…However, some studies reported the use of flow cytometry for the improvement of non-oleaginous fungal strains. [98][99][100][101][102] Successful isolation of fluorescent dye stained and/or fluorescent protein/tag expressed fungal spores and conidia was demonstrated in different non-oleaginous fungal strains including, Aspergillus fumigatus, Coniothyrium minitans, Trichoderma reesei, Trichoderma viridae, Penicillium expansum and Phoma herbarum. Green fluorescent protein (GFP) was the most frequently used fluorescent protein for the improvement of non-oleaginous fungal strains.…”

Section: Applications Of Flow Cytometry In Oleaginous Microorganismsmentioning

confidence: 99%

“…The study reported that FACS isolated recombinant T. reesei strain produced up to 2 mg hexadecanol per gram of biomass. A study by Throndset et al 99 reported enhanced secretion of industrially important enzyme glucoamylase in T. reesei strain, improved by flow cytometric sorting. A study by Bradner and Nevalainen 101 screened the T. reesei, P. expansum, and P. berbarum spores and hyphae using flow cytometry in combination with fluorescent dyes dihydroethidium (DHE), and hexidium iodide (HI).…”

Section: Applications Of Flow Cytometry In Oleaginous Microorganismsmentioning

confidence: 99%

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Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

Sonowal

Chikkaputtaiah

Velmurugan

2019

J of Chemical Tech & Biotech

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Oleaginous microorganisms, such as yeast, fungi, microalgae and bacteria, represent a key segment of second generation feed‐stock materials and are considered to synthesize a wide range of industrially important chemical compounds. Oleaginous microorganisms possess a broad varieties of chemical compounds such as carotenoids, pigments, carbohydrates, chlorophyll, and storage‐material lipids. Oleaginous microorganisms have been recognized as promising sources for the synthesis of unsaturated, especially polyunsaturated fatty acids (PUFA). So far, a variety of high‐throughput screening methodologies (HTMs) have been employed for the development of bioprocessing of oleaginous microorganisms for sustainable production of industrially valuable compounds. Of HTMs, flow cytometry (FC) and sorters (FACS) have received substantial interest as better HTMs because of their ability to screen large numbers of cells within seconds, and interrogate and isolate living cells at single‐cell level. Forward and side scattering signals of FC are used to determine the physiological state of the cell while different channels available in the FC facilitate the detection of signals produced from fluorophores. Simultaneous measurement of physiological characteristics along with specific compound accumulation at single‐cell level enables the possibility of separating a particular phenotype with specific properties from a population. Different microbial strain development strategies in combination with FACS produced improved phenotypes with desired properties. This review first summarizes the FACS methodologies suitable for oleaginous microorganisms and the significant progress that has been achieved in oleaginous microorganisms using FACS, and highlights the important, advanced and future prospects of FACS methodologies that are suitable for the development of bioprocessing in oleaginous microorganisms. © 2018 Society of Chemical Industry

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Fluorescent proteins in microbial biotechnology—new proteins and new applications

2011

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The recent advances over the past 5 years in the utilisation of fluorescent proteins in microbial biotechnology applications, including recombinant protein production, food processing, and environmental biotechnology, are reviewed. We highlight possible areas where fluorescent proteins currently used in other bioscience disciplines could be adapted for use in biotechnological applications and also outline novel uses for recently developed fluorescent proteins.

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Isolation of a strain of Trichoderma reesei with improved glucoamylase secretion by flow cytometric sorting

Cited by 7 publications

References 17 publications

Trichoderma reesei, a superior cellulase source for industrial applications

Trichoderma reesei, a superior cellulase source for industrial applications

Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

Fluorescent proteins in microbial biotechnology—new proteins and new applications

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