Isolation of a substance toxic to mitochondrial function from the burned skin of rats

A Novel Uncoupler of Mitochondrial Respiration, 9, 10-Epoxy-12-octadecenoate, Exists in Human Burned Skin

“…In previous papers, we reported extraction and primary characterization of `toxic substance' from burned skin of rats [6] and of humans [7]. In the present communication,…”

“…Measurement of mitochondrial respiration. Rat liver mitochondria were prepared as reported previously [6]. Oxygen consumption by mitochondria was recorded at 20°C using a Beckman 39550 oxygen electrode.…”

Mitochondrial dysfunction and mitophagy: crucial players in burn trauma and wound healing

“…Burn injury can cause significant tissue damage and releases harmful substances such as burn toxins, which can negatively impact mitochondrial function. Additionally, mitochondrial damage-associated molecular patterns (DAMPs) and pathogen-associated molecular pattern molecules (PAMPs) can also contribute to mitochondrial dysfunction [ 52 , 53 ]. DAMPs are biomolecules derived from damaged mitochondria that are released into the extracellular space and include proteins, DNA and lipids [ 54 ].…”

Improved ELISA for linoleate-derived diols in human plasma utilizing a polyHRP-based secondary tracer

“…4 EpOMEs were initially termed LTXs due to their cytotoxicity and association with poor outcomes in burn and acute respiratory distress syndrome (ARDS) patients and model species. [5][6][7] However, studies utilizing EH-decient systems and sEH inhibitors indicated hydrolytic bioactivation of LTXs is necessary for exertion of toxic effects, hence implicating the DiHOMEs (or LTXDs) as the deleterious agents. [8][9][10] DiHOMEs cause extensive vascular permeability 11 and are involved in inammatory diseases, [12][13][14][15] pulmonary damage, 16 sepsis, 17 peroxisomal disorders, 18 and burn injury, 19 with high concentrations closely correlating with worsening morbidities.…”