Isolation of Actinomycetes: A Complete Approach

Kumar, Ravi Ranjan; Jadeja, Vasantba J.

doi:10.20546/ijcmas.2016.505.062

Cited by 39 publications

(33 citation statements)

References 51 publications

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“…Actinomycetes were isolated from ultisol soil of marginal land at Cibinong Science Center following the methods described by Kumar and Jadeja (2016). The site was selected (6°29'26.0"S 106°51'05.0"E) due to the soil was nutrient deficient with the total carbon of 1.8% (w/w) and total nitrogen of 0.2% (w/w).…”

Section: Isolation Of Actinomycetesmentioning

confidence: 99%

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

et al. 2020

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Abstract. Sudiana A, Putri A, Napitupulu TP, Purnaningsih I, Idris, Kanti A. 2020. Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate. Biodiversitas 21: 429-435. Actinomycetes have been widely explored for new antibiotic production, but not many studies explore its abilities to inhibit the growth of phytopathogenic fungi and solubilize insoluble phosphate hence stimulate the growth of plants. We isolated Actinomycetes from the soil. Based on morphology, physiology, and 16S rDNA analyses, the isolate is closely related to Streptomyces sasae. The strain was able to inhibit the growth of phytopathogenic fungi Fusarium solani, and Fusarium oxysporum. S. sasae produced secondary metabolites 2-methyl-1,3-dioxolane as the major constituent. The strain assimilated variable carbon sources include L-arabinose, D-fructose, D-glucose, D-mannitol, Lactose, raffinose, L-rhamnose, and sucrose. The strain grew at pH 6.0 to 8.0, and at salinity (1-3%). Their growth was affected by the salinity level. The strain solubilized Ca-P at 1-3% salinity, but their ability to solubilize phosphate was influenced by salinity. The strain was also able to solubilize rock phosphate. Their ability to solubilize less soluble phosphate and inhibit the growth of F. solani and F. oxysporum may imply that this strain is potential for biocontrol agents. The 16S rRNA gene was submitted to DDBJ with the entry number 5df623c1a3c8820021322a36.TG01, and the accession number is LC514451.

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Section: Isolation Of Actinomycetesmentioning

confidence: 99%

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

et al. 2020

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“…The culture media used for the counting of the systematic groups was elective media modified by enrichment with 7% (v/v) waste extract and using spring water. The abundance of total bacteria, molds, yeasts and actinomycetes was estimated on their respective media: bacterial media [12], agar malt media, YPG (yeast, peptone and glucose) media, and media that was used by Kumar and Jadeja [13]. The microorganism count was carried out by the suspension-dilution method [12].…”

Section: Study Of Systematic Groupsmentioning

confidence: 99%

“…Our result is in disagreement with that found by Rana et al [32] and Aliero et al [33], who have shown that most actinomycetes are widely distributed in natural habitats such as aquatic and terrestrial habitats. Moreover, Kumar [13] and Rinoy et al [34] reported that the development of actinomycetes varies according to their mesophilic or thermophilic character, and their ability to degrade available substrates. The absence of actinomycetes at certain states of waste decay was due to physicochemical parameters, Adegboye et al [35], which reported that environmental factors such as temperature and humidity could affect the distribution of actinomycetes in an environment, support this in the study.…”

Section: Actinomycetesmentioning

confidence: 99%

Quantitative Research of Systematic and Functional Microbial Groups Associated with Decaying Solid Green Household Waste in Water and Soil

Barnossi¹,

Moussaid²,

Housseini³

2020

Pol. J. Environ. Stud.

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Currently, solid household waste worldwide is increasing as a result of population growth, changing production and consumption patterns [1-3]. This waste can cause a global problem due to its adverse effects on human health and the environment [1, 4, 5]. At the international level and especially in developing countries, biodegradable solid wastes are being used in agriculture as a bioresource through their environmental and economic benefits [6, 7]. Several research studies have focused on microbiological studies of household waste in the case of compost and vermicompost [8-10]. However, until now systematic and functional analyses of microbial groups of decaying green household waste in water and soil have been unsatisfactory [11]. Household

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“…The samples were pretreated in hot air oven at 70°C for 15 min. After which, 1 g of soil is serially diluted up to 10 −4 dilution and was plated on to the following isolation agar using spread plate technique: [11,12] Starch Casein Agar (SCA) -HiMedia, actinomycetes isolation agar -HiMedia, SCA -composed media (g/L: Starch: 10 g, casein: 0.3 g, KNO3: 2 g, NaCl: 2 g, K2HPO4: 2 g, MgSO4.7H2O: 0.05 g, CaCO3: 0.02 g, FeSO4.7H2O: 0.01 g, and agar: 18 g). [13] Humic acid vitamin agar -Composed media (Humic acid: 1 g, KCl: 1.7 g, Na2HPO4: 0.5 g, MgSO4: 0.5 g, CaCO3: 0.02 g, FeSO4: 0.01 g, VB stock solution: 1 ml, agar: 10 g, distilled water: 1000 mL, pH: 7.2 [VB stock solution: 50 mg VB1, VB2, niacin, VB6, D-calcium pantothenate, inositol, and PABA (para amino acid), 25 mg biotin, and 100 ml distilled water]) [14] and soil extracts agar -Composed media (peptone: 5 g, beef extract: 3 g, soil extracts: 1000 mL, agar: 10 g, and pH: 7.2).…”

Section: Isolation Of Actinomycetes From Marine Soil Samplementioning

confidence: 99%

Untitled

Каннабиран

2016

AJP

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Aim: To isolate and identify marine actinomycetes with potential antibacterial activity. Materials and Methods: Marine actinomycetes were isolated from soil samples collected at the Rameswaram and Dhanushkodi coast of Tamil Nadu, India. The isolated strains were screened for antibacterial activity by crossstreaking method against Escherichia coli and Bacillus cereus. Actinomycetes isolates demonstrated potential antibacterial activity were mass cultured and secondary metabolites were extracted using chloroform. The crude extract prepared was analyzed for antibacterial activity by well diffusion method (100 µg/0.1 ml) and also subjected to chemical characterization using gas chromatography-mass spectrometry (GC-MS) JEOL (GCMATE II GC-MS, Agilent Technologies 6890N Network GC system for GC). Results: Among the five different isolation media used, composed Starch Casein Agar (composed) proved to be most effective for isolation of actinomycetes from Rameswaram and Dhanushkodi coastal soil samples. Among the 100 isolated strains, 10 isolates (LGK001 to LGK010) demonstrated a significant antibacterial activity with the zone of inhibition ranging from 8 to 12 mm against Escherichia coli and B. cereus. Based on the GC-MS analysis, all 10 isolates produced either gancidin-W (and/or) cyclo-pro-phe as major secondary metabolites in their crude extracts. Both these molecules are previously reported antibiotic compounds. Conclusion: The results of this study suggest that Rameswaram and Dhanushkodi coastal soil samples are the rich sources for antibacterial bioactive compounds (gancidin-W and cyclo-pro-phe) producing actinomycetes.

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Isolation of Actinomycetes: A Complete Approach

Cited by 39 publications

References 51 publications

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

Quantitative Research of Systematic and Functional Microbial Groups Associated with Decaying Solid Green Household Waste in Water and Soil

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