Isolation of Ammonia Oxidizing Bacteria (AOB) from Fish Processing Effluents

Reddy, A. Devivaraprasad; Subrahmanyam, Gangavarapu; Kumar, Sekar Ashok; Karunasagar, Iddya; Karunasagar, Indrani

doi:10.1007/s40009-015-0363-7

Cited by 6 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, an appropriate protocol has to be adopted following former researchers for successful isolation of nitrifiers. However, it has also been noticed that various autotrophic ammonia oxidizers such as Nitrosomonas and Nitrosospira have been successfully isolated recently from various sources (Bollmann, French & Laanbroek, ; Reddy, Subrahmanyam, Naveenkumar, Karunasagar & Karunasagar, ) besides heterotrophic nitrifiers such as Bacillus sp. from membrane bioreactors (Lin et al., ; Zeng, Si & Li, ) and Alcaligenes faecalis from coke plant waste water (Liu, Li & Lv, ) where in 80% TN removal has been observed.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

et al. 2017

View full text Add to dashboard Cite

Nitrate reducing potency of 88 bacterial isolates segregated from marine and brackish water nitrifying bacterial consortia (NBC), generated for activation of nitrifying bioreactors, was confirmed by determining the nitrate reducing capability under aerobic conditions as maintained in nitrifying bioreactors. All the isolates had the potential to be used as bio‐augmentors for activating nitrate dissimilation in recirculating aquaculture system. The existence of nitrate reducers with nitrifiers in NBC and in the reactor configuration negates the requirement of integrating anoxic denitrifying system for effective removal of NO3−‐N. Phylogenetic analyses of representative isolates from each cluster of the dendrograms generated based on phenotypic characterization and amplified ribosomal DNA restriction analysis revealed profound diversity of nitrate reducing bacterial flora in the NBC. They were composed of Streptomyces enissocaesilis, Marinobacter sp., Pseudomonas sp., Microbacterium oxydans, Pelagibacterium halotolerans and Alcanivorax dieselolei from marine NBC and Streptomyces tendae, Nesterenkonia sp., Bacillus cereus, Microbacterium oxydans and Brevibacterium sp. from brackish water NBC. The diversity indices of the consortia were calculated using Mega 5.0, primer 7 and VITCOMIC softwares. Marine NBC exhibited higher Shannon wiener diversity and mean population diversity than brackish water NBC. The study delineated higher species richness and diversity in marine NBC than in its brackish water counterpart, a possible reflection of the higher biodiversity of marine systems, and hence, the former is more promising to be used as start‐up cultures for the activation of nitrifying bioreactors after appropriate acclimatization to the desired salinity.

show abstract

Section: Resultsmentioning

confidence: 99%

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In recent years, autotrophic and heterotrophic AOBs in water and soil have been studied (Matsuno et al, 2013; Soliman & Eldyasti, 2018). In soil ecosystems, except for autotrophic AOB strains, some heterotrophic AOB strains such as Pseudomonas , Ochrobactrum , Paracoccus , and Acinetobacter play crucial roles in soil nitrification (Kouki et al, 2011; Matsuno et al, 2013; Reddy et al, 2014; Wang et al, 2021). The present study isolated and screened the AOB strains S2_8_1 and S2_7_25, which belong to Ensifer and Acinetobacter , have various nutritional characteristics.…”

Section: Discussionmentioning

confidence: 99%

Increasing Italian ryegrass (Lolium multiflorum Lam.) regrowth via inoculation with an ammonia‐oxidizing bacterial strain

Wang

et al. 2022

Grassland Science

View full text Add to dashboard Cite

This study aimed to investigate the effect of a soil ammonia‐oxidizing bacterial (AOB) strain on Italian ryegrass (Lolium multiflorum Lam.) regrowth. Potted Italian ryegrass was used as test material. We isolated and screened an AOB strain from the soil for use in inoculation of other soils to increase nitrification. The sequence of the two isolated and screened AOB strains (S2_8_1 and S2_7_25) were 100% similar to that of Ensifer sesbaniae and Acinetobacter pittii. The nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) was also added to the soil to inhibit nitrification. The experimental design involving the inoculation of the soils with the AOB strain included seven treatments: (1) regrowth without any additives, (2) regrowth with blank enrichment medium added to the soil, (3) regrowth with S2_8_1 strain inoculation, (4) regrowth with S2_7_25 strain inoculation, (5) regrowth with DMPP added to the soil, (6) regrowth with DMPP added to the soil and with S2_8_1 strain inoculation, and (7) regrowth with DMPP added to the soil and with S2_7_25 strain inoculation. Compared with regrowth treatment without any additives, the regrowth treatment with S2_8_1 and S2_7_25 inoculations significantly increased leaf biomass by 23.5–48.6%, the leaf net photosynthetic rate by 27.9–48.5%, the rhizosphere soil nitrification rate by 43.0–144.2%, leaf cytokinin concentrations by 23.7–24.9%, and xylem sap cytokinin concentrations by 32.5–43.2%. The increase of nitrate nitrogen content in rhizosphere soil induced by S2_8_1 and S2_7_25 promoted the transfers of cytokinin from roots to leaves, resulting in the increase of cytokinin concentration in leaves. This increase in leaf cytokinin concentrations improved Italian ryegrass regrowth. However, the S2_8_1 and S2_7_25‐induced increases in the nonrhizosphere soil nitrification rate were not closely related to Italian ryegrass regrowth. The tested AOB strains have considerable potential to increase Italian ryegrass regrowth.

show abstract

“…These strains are identified as Nitrosomonas nitrosa and N. marina of Betaproteobacteria, respectively by 16S rRNA gene sequencing analysis (Reddy et al, 2015). Consortia culture was prepared by mixing of AOB-12 and AOB-21 culture in the ratio of 1:1.…”

Section: Culturesmentioning

confidence: 99%

Effect of Ammonia Concentration on the Nitrification Potential of Ammonia Oxidizing Bacterial Isolates from Fish Processing Waste Effluents

Reddy¹,

Subrahmanyam²,

Dechamma³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

Self Cite

View full text Add to dashboard Cite

Isolation of Ammonia Oxidizing Bacteria (AOB) from Fish Processing Effluents

Cited by 6 publications

References 18 publications

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

Increasing Italian ryegrass (Lolium multiflorum Lam.) regrowth via inoculation with an ammonia‐oxidizing bacterial strain

Effect of Ammonia Concentration on the Nitrification Potential of Ammonia Oxidizing Bacterial Isolates from Fish Processing Waste Effluents

Contact Info

Product

Resources

About