Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain

Herrero, Cláudia; Peláz, Carmen; Martin-Bourgon, C

doi:10.1017/s0950268800050056

Cited by 6 publications

(4 citation statements)

References 7 publications

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“…(22). Previous reports of R. rhipicephali in Europe and Africa were based on PCR-restriction fragment length polymorphism profiles compatible with R. rhipicephali after digestion of ompA PCR products obtained from ticks (2,11,16). Thus, further studies based on isolation in cell culture and/or DNA sequencing of PCR products need to be conducted to confirm the presence of R. rhipicephali outside the New World.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of São Paulo, Brazil

Labruna

Pacheco

Richtzenhain

et al. 2007

Appl Environ Microbiol

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In the present study, attempts to isolate Rickettsia in cell culture were performed individually in seven specimens of Haemaphysalis juxtakochi ticks collected in the state of São Paulo (southeastern Brazil). Rickettsia was successfully isolated by the shell vial technique and established in Vero cell culture from six ticks (six isolates). DNA extracted from infected cells of these isolates was tested by PCR and DNA sequencing, using genus-specific Rickettsia primers targeting the genes gltA, htrA, ompA, and ompB. After the generated sequences were compared with available sequences in GenBank, five out of the six isolates were identified as Rickettsia bellii (isolates HJ#1, HJ#2, HJ#3, HJ#4, and HJ#7). The sixth isolate (HJ#5) was closest to Rickettsia sp. strain R300, previously detected in H. juxtakochi in northern Brazil, and to Rickettsia rhipicephali, isolated from ticks in the United States. Following recent gene sequence-based criteria proposed for the identification of Rickettsia isolates, both isolate HJ#5 and strain R300 were identified as South American strains of R. rhipicephali, which was confirmed in this continent for the first time. Isolation of R. bellii from H. juxtakochi ticks, added to eight other tick species that have been reported to be infected with this bacterium in Brazil, indicates that R. bellii is indeed the most frequent Rickettsia species infecting ticks in Brazil. Currently, the role of both R. rhipicephali and R. bellii as human pathogens is regarded as unknown.

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Section: Discussionmentioning

confidence: 99%

Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of São Paulo, Brazil

Labruna

Pacheco

Richtzenhain

et al. 2007

Appl Environ Microbiol

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“…Reports dealing with the resurgence of MSF in Spain in the early 1980s (31,33,45) have been behind the origin of several clinical studies of the disease and serosurveys in humans and dogs (16,20,22,25,31,(42)(43)(44)(45)(46). So far, the two strains isolated in Spain have been identified as R. conorii in Catalonia and R. rhipicephali in the region of Madrid (17,23,39). For a better knowledge of the epidemiology of SFG rickettsioses in Spain, more rickettsial isolates are needed in order to identify them and compare them with reference strains.…”

Section: Discussionmentioning

confidence: 99%

“…However, by pulsed field gel electrophoresis (PFGE), one of them, the 16B strain, has been found to be identical to R. conorii (39). The only tick isolate obtained in Spain by Vero cell inoculation with eggs of R. sanguineus from dogs near Madrid has been identified, by PCR-restriction fragment length polymorphism (PCR-RFLP) only, as Rickettsia rhipicephali (23). SFG rickettsiae genotypically different from R. conorii have been detected in or isolated from the hemolymph of ticks belonging to the R. sanguineus complex in other Mediterranean countries (1-3, 7, 14).…”

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Phenotypic and genotypic characterization of spotted fever group Rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks

et al. 1996

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Eighty-nine Rhipicephalus sanguineus ticks and 21Rhipicephalus bursa ticks collected in Catalonia were tested by the hemolymph test to establish their infection rate with spotted fever group rickettsiae. By Giménez staining, 11.2% of the R. sanguineus isolates and 0% of the R. bursa isolates were found to contain rickettsia-like organisms. Six spotted fever group rickettsial strains (Bar29, Bar31, Gir4, Tar1, Tar2, and Tar3) were isolated from these ticks and were characterized by phenotypic and genotypic analyses. PCR followed by restriction fragment length polymorphism analysis showed that the six strains were identical and were characterized by the same restriction profiles as a strain, Mtu5, previously isolated from Rhipicephalus turanicus ticks in the South of France. Microimmunofluorescence serotyping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified organisms, and Western blot (immunoblot) assay with mouse polyclonal sera confirmed this observation. Pulsed field gel electrophoresis of the whole genome of three of the strains showed that, although closely related, the profile of Tar1 was slightly different from that of the Bar strains. Phylogenetic analysis showed that this new rickettsial sero-and genotype, which will be named the "Catalan strain," is closely related to Rickettsia massiliae. This strain shows an unexpected resistance to rifampin. The epidemiological implications of these findings are considered.

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“…In Spain, the epidemiology of rickettsiae and rickettsial diseases is still poorly described, although several MSF seroepidemiologic surveys [1][2][3] and clinical studies of this disease 4,5 have been reported. The first tick rickettsial isolate was obtained in Spain by Vero cell inoculation with eggs of Rhipicephalus sanguineus isolated from dogs near Madrid and identified by polymerase chain reaction (PCR)-restriction fragment length polymorphism as R. rhipicephali, 6 an SFG rickettsia that seems to be nonpathogenic for humans. In Catalonia, Spain, the first 2 SFG rickettsial strains isolated from humans differed in cytopathogenicity from the reference R. conorii Moroccan strain, 7 although 1 of them, the 16B strain, was identical to R. conorii by pulsed-field gel electrophoresis.…”

mentioning

confidence: 99%

Short report: isolation and identification of two spotted fever group rickettsial strains from patients in Catalonia, Spain.

Cardeñosa

Roux²,

Font³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. Two rickettsial strains, 16B (previously isolated) and FB1, were isolated from blood from patients with Mediterranean spotted fever in Catalonia, Spain. These are the only 2 human rickettsial isolates of the spotted fever group obtained so far in Spain. These strains were identified by the polymerase chain reaction and sequence analysis of a fragment of the outer membrane protein A (ompA) gene. The partial ompA sequence was found to be 100% identical with that of Rickettsia conorii (Malish 7 strain) for both strains. These results confirm the presence of R. conorii in Catalonia, despite the fact that in a previous study, no R. conorii were isolated, but a new rickettsial strain of the spotted fever group (Bar29) was isolated from dog ticks (Rhipicephalus sanguineus) in Catalonia. Further studies are necessary to get a better knowledge of the epidemiology of rickettsiae in Catalonia.

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Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain

Cited by 6 publications

References 7 publications

Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of São Paulo, Brazil

Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of São Paulo, Brazil

Phenotypic and genotypic characterization of spotted fever group Rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks

Short report: isolation and identification of two spotted fever group rickettsial strains from patients in Catalonia, Spain.

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