Isolation of an amikacin-resistant Escherichia coli strain after tobramycin treatment of previous recurrent episodes of respiratory tract infections caused by Pseudomonas aeruginosa

Ruiz, Joaquı́m; Bertran, S.; Sauca, Goretti; Julià, Antoni; Vila, Xavier Sánchez; Gómez, Esperanza Bautista; Anta, M. T. Jiménez de; Vilà, Jordi

doi:10.1111/j.1469-0691.2004.01039.x

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Overall, it seems that the presence of both adenylyltransferase ( aadA ) and acetyltransferase ( aacA4 ) is essential for amikacin resistance. Previous studies related amikacin resistance in E. coli to the presence of aacA4 and aacA7 together [ 40 ]; along the same lines with our finding, it was reported that the ANT(3″) enzymes confer more resistance against amikacin in Serratia marcescens by increasing the affinity of the aminoglycoside to bind AAC(6′) enzyme [ 41 ]. Only one isolate (1/18) was integron I-negative and aacA4 -positive, and it showed resistance to amikacin; in this isolate, other mechanisms could play a role in such resistance.…”

Section: Discussionsupporting

confidence: 89%

The Link between Occurrence of Class I Integron and Acquired Aminoglycoside Resistance in Clinical MRSA Isolates

et al. 2021

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Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections because of its high resistance. Here, we study the antibiotic resistance in MRSA clinical isolates and their relation to integron I occurrence. A total of 88 clinical Staphylococcusaureus isolates were collected. MRSA were identified by the disk diffusion method (DDM) and confirmed by PCR, and antibiogram was determined by DDM. Integron I, II and the aacA4 gene were investigated by PCR. Integrase-positive strains were analyzed for the presence of resistance gene cassettes by sequencing. All isolates were identified as MRSA by DDM and confirmed by PCR. All isolates were resistant to ampicillin and cefoxitin. Concerning aminoglycosides, the frequency of resistance was reported for streptomycin (60.7%), tobramycin (37.1%) gentamicin (36%), and for amikacin (15.9%). Integron I was detected in 41 isolates (46.6%), while integron II was detected in three isolates (3.4%). Sequencing of the integron I-cassette indicated the exclusive prevalence of addA gene variants mediating aminoglycoside resistance. The aacA4 gene was found in DNA of 31 isolates (35.22%). This study revealed the high existence of MRSA. Furthermore, the AacA4 gene and class I integron harboring aadA gene were predominant in MRSA isolates.

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Section: Discussionsupporting

confidence: 89%

The Link between Occurrence of Class I Integron and Acquired Aminoglycoside Resistance in Clinical MRSA Isolates

et al. 2021

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“…Additionally, the resistance of amikacin against most aminoglycoside-modifying genes is commonly reported (as reviewed in [ 81 ]). Notably, amikacin resistance is reported to be mediated by aminoglycoside genes such as aphA6 [ 82 ], armA [ 83 ], aacA4 and aacA7 [ 84 ], none of which were detected in the Segamat cohort.…”

Section: Discussionmentioning

confidence: 99%

Pan-genome and resistome analysis of extended-spectrum ß-lactamase-producing Escherichia coli: A multi-setting epidemiological surveillance study from Malaysia

et al. 2022

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Objectives This study profiled the prevalence of extended-spectrum ß-lactamase-producing Escherichia coli (ESBL-EC) in the community and compared their resistome and genomic profiles with isolates from clinical patients through whole-genome sequencing. Methods Fecal samples from 233 community dwellers from Segamat, a town in southern Malaysia, were obtained between May through August 2018. Putative ESBL strains were screened and tested using antibiotic susceptibility tests. Additionally, eight clinical ESBL-EC were obtained from a hospital in the same district between June through October 2020. Whole-genome sequencing was then conducted on selected ESBL-EC from both settings (n = 40) for pan-genome comparison, cluster analysis, and resistome profiling. Results A mean ESBL-EC carriage rate of 17.82% (95% CI: 10.48%– 24.11%) was observed in the community and was consistent across demographic factors. Whole-genome sequences of the ESBL-EC (n = 40) enabled the detection of multiple plasmid replicon groups (n = 28), resistance genes (n = 34) and virulence factors (n = 335), with no significant difference in the number of genes carried between the community and clinical isolates (plasmid replicon groups, p = 0.13; resistance genes, p = 0.47; virulence factors, p = 0.94). Virulence gene marker analysis detected the presence of extraintestinal pathogenic E. coli (ExPEC), uropathogenic E. coli (UPEC), and enteroaggregative E. coli (EAEC) in both the community and clinical isolates. Multiple blaCTX-M variants were observed, dominated by blaCTX-M-27 (n = 12), blaCTX-M-65 (n = 10), and blaCTX-M-15 (n = 9). The clinical and community isolates did not cluster together based on the pan-genome comparison, suggesting isolates from the two settings were clonally unrelated. However, cluster analysis based on carried plasmids, resistance genes and phenotypic susceptibility profiles identified four distinct clusters, with similar patterns between the community and clinical isolates. Conclusion ESBL-EC from the clinical and community settings shared similar resistome profiles, suggesting the frequent exchange of genetic materials through horizontal gene transfer.

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“…These results were agreed with Niranjan and Malini who found the isolates of this bacteria were sensitive to this antibiotic (Niranjan and Malini., 2014). But in study of Ruiz et al who found some isolates of this bacteria were resistant to this antibiotic (Ruiz et al, 2005). The current study explained the (Bacitracin) antibiotic has low activity against Escherichia coli.…”

Section: Discussionmentioning

confidence: 53%

Isolation, Identification and Antibiotic Susceptibility of pathogenic Bacteria Isolated from Clinical Samples

Hussein¹

2016

IOSR

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150 Samples have been collected, through the period from January/2016 to June/2016. This for isolation and identification of pathogenic gram negative bacteria. The samples were involve 50 burns samples for isolate Pseudomonas aeruginosa, 50 stool samples for isolate Escherichia coli and 50 urine samples for isolate Enterobacter cloacae and Proteus mirabilis. The isolates were identified by ten biochemical tests as well as the sensitivity and resistant were tested by thirteen type of antibiotics. These antibiotics explained different mode of action in their activity, were resistant, moderate and sensitive, some of them have significant differences when comparison together and some have no. The Trimethoprim, Amikacin, Aztreonam and Novobiocin have high activity against Ps. aeruginosa, E. coli, E. cloacae and P. mirabilis respectively, as well as the Aztreonam was more active when comparison the activity of these antibiotics together.

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Isolation of an amikacin-resistant Escherichia coli strain after tobramycin treatment of previous recurrent episodes of respiratory tract infections caused by Pseudomonas aeruginosa

Cited by 6 publications

References 16 publications

The Link between Occurrence of Class I Integron and Acquired Aminoglycoside Resistance in Clinical MRSA Isolates

The Link between Occurrence of Class I Integron and Acquired Aminoglycoside Resistance in Clinical MRSA Isolates

Pan-genome and resistome analysis of extended-spectrum ß-lactamase-producing Escherichia coli: A multi-setting epidemiological surveillance study from Malaysia

Isolation, Identification and Antibiotic Susceptibility of pathogenic Bacteria Isolated from Clinical Samples

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